ID A0A1Q8Q2P4_9BACI Unreviewed; 908 AA.
AC A0A1Q8Q2P4;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Aconitate hydratase {ECO:0000256|RuleBase:RU361275};
DE Short=Aconitase {ECO:0000256|RuleBase:RU361275};
DE EC=4.2.1.3 {ECO:0000256|RuleBase:RU361275};
GN ORFNames=BTO30_13880 {ECO:0000313|EMBL:OLN21572.1};
OS Domibacillus antri.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Domibacillus.
OX NCBI_TaxID=1714264 {ECO:0000313|EMBL:OLN21572.1, ECO:0000313|Proteomes:UP000185568};
RN [1] {ECO:0000313|EMBL:OLN21572.1, ECO:0000313|Proteomes:UP000185568}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=XD80 {ECO:0000313|EMBL:OLN21572.1,
RC ECO:0000313|Proteomes:UP000185568};
RA Verma A., Krishnamurthi S.;
RT "Domibacillus antri genome sequencing.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-
CC aconitate. {ECO:0000256|RuleBase:RU361275}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501,
CC ECO:0000256|RuleBase:RU361275};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185, ECO:0000256|RuleBase:RU361275}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLN21572.1}.
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DR EMBL; MSDU01000038; OLN21572.1; -; Genomic_DNA.
DR RefSeq; WP_075399318.1; NZ_MSDU01000038.1.
DR AlphaFoldDB; A0A1Q8Q2P4; -.
DR STRING; 1714264.BTO30_13880; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000185568; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01586; AcnA_IRP; 1.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01341; aconitase_1; 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|RuleBase:RU361275};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU361275};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU361275};
KW Lyase {ECO:0000256|RuleBase:RU361275};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532}.
FT DOMAIN 76..572
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 702..828
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 908 AA; 99218 MW; C7C49433EA45EFD4 CRC64;
MAKNDVFNAR ASFDLNGKRY NYYRLAALEE AGTAKISRLP YSIKVLLESV LRQYDGYVIK
KDHVENLAKW GVDADKEAEV PFKPSRVILQ DFTGVPVVVD LASLRKAMAD IGGDPQVINP
EIPVDLVIDH SVQVDAYGTP EALERNMELE FDRNAERYEF LSWAQKAFDN YRAVPPATGI
VHQVNLEYLA SVVHAVETTE GEFETYPDTL VGTDSHTTMI NGIGVLGWGV GGIEAEAGML
GQPSYFPIPE VVGVKLTGEL PDGATATDLA LKVTQILRQQ GVVGKFVEFF GAGVSTLPLA
DRATIANMAP EYGATCGFFP VDAEALDYMR LTGRDEDHIQ VVEQYLKENG MFFTSANEDP
IYTEVVELNL AEIEPNLSGP KRPQDLIPLS QMKKSFQEAV SAPQGNHGFG LTADELDRTT
TVTFNDGTTA EMKTGAIGIA AITSCTNTSN PYVLLAAGLV AKKAVELGLQ VPKYVKTSLA
PGSKVVTGYL RDSGLLPYME QIGFNVVGYG CTTCIGNSGP MKEEIEKAII DSDLLVTSVL
SGNRNFEGRI HPLVKGNYLA SPPLVVAYAL AGNVNVDLKN EPIGKGKDGQ DVFFKDIWPG
TEEVKEVVKR TVTPELFRKE YEQVFNSNAK WNQIQTSNEA LYSWDEDSTY IANPTFFQNL
SPNPEPIQAL TGLRVMGKFG DSVTTDHISP AGAIGKDTPA GLYLRSKGVE PRDFNSYGSR
RGNHEVMMRG TFANIRIRNQ IAPGTEGGFT TFWPTGEVMP IYDACMKYQE QGTGLVVLAG
KDYGMGSSRD WAAKGTNLLG VKTVIAESYE RIHRSNLVMM GVLPLQFKLG ENATTLGLTG
EETIEVAIND NVKPREVVKV TATDKDGKKT EFEALVRFDS EVDVDYYRHG GILQMVLRGK
LQSVNAGK
//
