ID A0A1Q9CWR0_SYMMI Unreviewed; 2455 AA.
AC A0A1Q9CWR0;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE SubName: Full=Pullulanase 1, chloroplastic {ECO:0000313|EMBL:OLP87329.1};
GN Name=PU1 {ECO:0000313|EMBL:OLP87329.1};
GN ORFNames=AK812_SmicGene31456 {ECO:0000313|EMBL:OLP87329.1};
OS Symbiodinium microadriaticum (Dinoflagellate) (Zooxanthella
OS microadriatica).
OC Eukaryota; Sar; Alveolata; Dinophyceae; Suessiales; Symbiodiniaceae;
OC Symbiodinium.
OX NCBI_TaxID=2951 {ECO:0000313|EMBL:OLP87329.1, ECO:0000313|Proteomes:UP000186817};
RN [1] {ECO:0000313|EMBL:OLP87329.1, ECO:0000313|Proteomes:UP000186817}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP2467 {ECO:0000313|EMBL:OLP87329.1,
RC ECO:0000313|Proteomes:UP000186817};
RA Aranda M., Li Y., Liew Y.J., Baumgarten S., Simakov O., Wilson M., Piel J.,
RA Ashoor H., Bougouffa S., Bajic V.B., Ryu T., Ravasi T., Bayer T.,
RA Micklem G., Kim H., Bhak J., Lajeunesse T.C., Voolstra C.R.;
RT "Genome analysis of coral dinoflagellate symbionts highlights evolutionary
RT adaptations to a symbiotic lifestyle.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLP87329.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LSRX01000866; OLP87329.1; -; Genomic_DNA.
DR OrthoDB; 1326984at2759; -.
DR Proteomes; UP000186817; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd02854; E_set_GBE_euk_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 3.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR008615; FNIP.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR024561; Pullul_strch_C.
DR PANTHER; PTHR43651; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR PANTHER; PTHR43651:SF2; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME, CHLOROPLASTIC_AMYLOPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF05725; FNIP; 1.
DR Pfam; PF11852; Pullul_strch_C; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 2.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000186817}.
FT DOMAIN 791..946
FT /note="Alpha-1,6-glucosidases pullulanase-type C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11852"
FT DOMAIN 1023..1104
FT /note="Glycoside hydrolase family 13 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02922"
FT DOMAIN 2309..2397
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|Pfam:PF00128"
FT REGION 12..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1224..1256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1526..1778
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 13..28
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1228..1256
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1545..1560
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1569..1583
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1601..1778
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2455 AA; 271250 MW; 455D99DA5A5F1200 CRC64;
MGIALNCKFD FGTVPERPED RAEPMMPKKA GPDSEGQQLA VMEVADKDAC MSTPCRRPQE
AAARKNGSLP AGDLCRDFGR TATGGFVLLG APLLVTAASK EGDVQIAALD KAAGALVAAD
PDNPSFVEQS DVEEDTDIPR FHLERPRSRA RDFRDTVIGD ATSPKTDVQF RWIKRDGEAY
RMQMRVPKSD GGGMKEGGTF SRREDAVDAA KGPNFLISEA GGRPSSHGTS ETGQLLLPAS
KPARRGSLAW FVLLLWKRLL DENTLSDADV AALDEMMWYS GRELSQNIRL PGSLQSLTFG
YVFNQSLEDI QLPSSLQRLT FGYAFDQRLR ASNYRARFDV WLCVQPAFNW GYDPVLYGVP
EGSYSSDPDG MARVLEHRRM VAGLHKKGLR VIADVVFNHV FGSGPKGNHS VLDKHLGDPC
ELFRTLRVHH VDLWSKSILI QSNLERLVVD MIRHWAIEHR MDGFRFDLMG HITLECIQKC
RAALDELSVE EHGIDGPRLL LYGEGWEFGE IEGGARGATA CQRQLAGTGI GSFNDNLRGA
VLGGNAFEDP RLQGFATGLG LREPSDVALK TTTSELELLV AFGRDIWRED PHTPGIYEYK
DTKDYRGPQR VVDKSSWERV REEEVEDEKA VSKGLGRTSP LGRRRRRPVA PALLGGDDFF
RKFTTGATGE LSLNGDPMAN SESAQRASVH GGKAAYVSSP EEVVNFICVH DNETLYDSTI
WKMSPETTDP EERMRANWLC SATLALGHGV PLFHAGDEVL RSKSLDRDSY NSGDWFNLLD
YSGETSAFGA GNHTKWELMK PLLRDDRLRP NKEMVAATTA KFCELLGIRK STALLGLREA
ADIVDKVHFP GCGLGQIPGV VVMEVRNGTS SASGTPLCDK YSRVVVVFSA RPDEVRIPAP
TSRSGGMLML HPLQVASADT RTREARYDEA TDELVVPALT AAVFVEPQPN QPSFKASERR
AQFRPALRER PIVGDGMGLV AADPACVPFK GHFEYRWKVY TDLRKHISET SGSLTEFASG
FRKLGFTKDE ASGAITYREW LPPAKAVSLV GDFNNWDEGA TPLARDAFGT WEVTLPAKSI
AHRTKVKFCA TTPDGSKVYR VPAWIRWSCP DLGKFGSTYD GYYWDPSEDV LSPDELAIVV
QNYRDGGLNA QPETPEKEEA PRKKGSLLEW EHPSKVGLIN RRSLMLNWKV TRFFEEIEDH
RLKAIFDEVA KSWPPKIRSR KTLVVAEEDG EIEDEQEEEE EEEQQLTEDD EEDGQPAEVD
GYLAQSLGIV PGSPAPPASA YVATVAYELS DEGPPVPYAA ALPNGDDDTD LVRQLAELEQ
QIAHRRAALS GRQAIERWHS LQHMDSQRTI AWPETLQEFV AEQPAPLSSA TEGGYFRQGA
SSNLSLGHVE AKDVAPCNVP VATPARAASV APAVSEDLAD PTDVAVAPGV SEDLADPTKA
AVAPGVSEDL ADKAAVAPGV SEDLADKAPV APGVSEDLAE PAVAPGVSQD LADPAQLAVA
PGVLKDLADR AVAPGVLKDL ADPVAPRVSD DLGEATPATP RPESVSLEIA SSSQPPSPEL
LALKVQTRRA EQMRGREGGT QEDVPVFKRP AAKGWPKGKA KARPAKGELL DDEPTKRAKK
GQQGDDEPKK RATKGEQGDD EPKPKRATKG EQQGDEPKPK RAKKGEQGDE PKPKRAKKGE
QQGEEEPKPK RAKKGKQGDD EPKRAKKGEQ QGDDEPKRAK KGKQGDEPKR AQKGKQGDEP
KRAQKGKQGD EEPKPKPAKK GKQGDEPKPL KKTFAKRWEP EVEGRSKCLW TSIRDAFNDL
IRPQLRFPTK FEDPFWMHCR PKLIELEEAT AKDTKRCINQ MQTPTQPEEK RRNDSLEFQL
EAYWMAYRQT HSTHPVFGRG LDLGTTIPLA FYSDEGKGPK RGNFVVVAIE SPIGLMDVAD
DFTCTCQEDV KKAPQQFVPG QQAAGPYTPM EEAALKQNTT CKGHSYLTRH VLFGLPDWIY
KHHPEVLEKM TQQVADELTT LFSSGLEING KVYTACLVGI KGDLKQIAEK IAYLNRYYAR
LGPVSYNDIC GSLVLLARLG YYDDPNGGDE TNLRARLIRC FQHFRLWRLA AGKSAAVRYF
SASLFNLKRL SDFAWSNTKG SDTMLLLEYL SFYLTILLRR PNLPAAHAVM FRVLKKTIGE
SQKAFNLMYK HGLWLRRTCA QNLYLRLMSV LSGYQHLAQH ALTMGMTFFA LKPKYHAIHH
VAYELRVALL TEAKLIPNPI TWNCEMGEDL IGRICALSLK AGKEEYCFEG FQAARPSLPS
TLRIYEAHVG MSSTEPKCNS YLEFAKDVLP RIKEAGYNCV QLMAIMEHSY YGSFGYHVTS
PFAVSSRSGT PDEFKALVEE AHRLGMLVLI DLVHSHVSNN ADDGLNGMDF GQAESESYFM
TGERGYHKLW DSRVVLPMSA TAKQDTGGRE RQTAGCTPHT ADIQTQLCLQ HSHVQ
//
