ID A0A1Q9GEF8_9GAMM Unreviewed; 434 AA.
AC A0A1Q9GEF8;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE RecName: Full=C4-dicarboxylate transporter {ECO:0000256|PIRNR:PIRNR004539};
GN ORFNames=BIT28_06200 {ECO:0000313|EMBL:OLQ72781.1};
OS Photobacterium proteolyticum.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Photobacterium.
OX NCBI_TaxID=1903952 {ECO:0000313|EMBL:OLQ72781.1, ECO:0000313|Proteomes:UP000186905};
RN [1] {ECO:0000313|EMBL:OLQ72781.1, ECO:0000313|Proteomes:UP000186905}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=13-12 {ECO:0000313|EMBL:OLQ72781.1,
RC ECO:0000313|Proteomes:UP000186905};
RA Li Y.;
RT "Photobacterium proteolyticum sp. nov. a protease producing bacterium
RT isolated from ocean sediments of Laizhou Bay.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for the transport of C4-dicarboxylates.
CC {ECO:0000256|PIRNR:PIRNR004539}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate(in) + succinate(out) = (S)-malate(out) +
CC succinate(in); Xref=Rhea:RHEA:29327, ChEBI:CHEBI:15589,
CC ChEBI:CHEBI:30031; Evidence={ECO:0000256|ARBA:ARBA00034284};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:29329;
CC Evidence={ECO:0000256|ARBA:ARBA00034284};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-aspartate(in) + succinate(out) = L-aspartate(out) +
CC succinate(in); Xref=Rhea:RHEA:29343, ChEBI:CHEBI:29991,
CC ChEBI:CHEBI:30031; Evidence={ECO:0000256|ARBA:ARBA00034237};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:29345;
CC Evidence={ECO:0000256|ARBA:ARBA00034237};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=fumarate(in) + L-aspartate(out) = fumarate(out) + L-
CC aspartate(in); Xref=Rhea:RHEA:72459, ChEBI:CHEBI:29806,
CC ChEBI:CHEBI:29991; Evidence={ECO:0000256|ARBA:ARBA00036117};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72460;
CC Evidence={ECO:0000256|ARBA:ARBA00036117};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=fumarate(in) + succinate(out) = fumarate(out) + succinate(in);
CC Xref=Rhea:RHEA:29323, ChEBI:CHEBI:29806, ChEBI:CHEBI:30031;
CC Evidence={ECO:0000256|ARBA:ARBA00034287};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:29325;
CC Evidence={ECO:0000256|ARBA:ARBA00034287};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004429, ECO:0000256|PIRNR:PIRNR004539}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004429,
CC ECO:0000256|PIRNR:PIRNR004539}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the DcuA/DcuB transporter (TC 2.A.13.1) family.
CC {ECO:0000256|ARBA:ARBA00006413, ECO:0000256|PIRNR:PIRNR004539}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLQ72781.1}.
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DR EMBL; MJIL01000090; OLQ72781.1; -; Genomic_DNA.
DR RefSeq; WP_075766797.1; NZ_MJIL01000090.1.
DR AlphaFoldDB; A0A1Q9GEF8; -.
DR STRING; 1903952.BIT28_06200; -.
DR OrthoDB; 9157063at2; -.
DR Proteomes; UP000186905; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015556; F:C4-dicarboxylate transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR004668; Anaer_Dcu_memb_transpt.
DR NCBIfam; TIGR00770; Dcu; 1.
DR PANTHER; PTHR36106:SF2; ANAEROBIC C4-DICARBOXYLATE TRANSPORTER DCUA; 1.
DR PANTHER; PTHR36106; ANAEROBIC C4-DICARBOXYLATE TRANSPORTER DCUB; 1.
DR Pfam; PF03605; DcuA_DcuB; 1.
DR PIRSF; PIRSF004539; C4-dicrbxl_trns; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|PIRNR:PIRNR004539};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475,
KW ECO:0000256|PIRNR:PIRNR004539};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|PIRNR:PIRNR004539};
KW Reference proteome {ECO:0000313|Proteomes:UP000186905};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|PIRNR:PIRNR004539}.
FT TRANSMEM 7..40
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 46..64
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 163..187
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 229..246
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 290..308
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 328..352
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 364..389
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 409..433
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
SQ SEQUENCE 434 AA; 45428 MW; 586C03416A952992 CRC64;
MIFIQTCLLL GAIILAARLG GIGVGLAGGL GMAIAVFGLG LPPGSIPVSV ILIIMSVILA
LSVMQQAGGM SYMVNCAEKM LRNHPKYINI LAPATTFVLT TLAGTGYTAM SVLNVIQQVA
KENGVRPSQP LSSAVVASQI AITASPISAA TAAMYVVVEQ MGVSFGTALS VIMPSALFGV
VVASFIASRQ GCELKDDPIF KERVAKGLVN FTPEEQKQIE PSIEAKRSVW LFLGGVMFIV
ALLLFKPLLG HDLGSRDIIV IVMLLTSFIM AMVCKVQLTS IKKAPIFADG AESLIVILGI
VWLSSTIIGV HIPEIKAIAG DVLSEYPALL AVVFFGTSAL LFSQGATSAL LVPIAASLNV
DPGTILASFV AVSALYMTNI YPTTAFAIAT DDTGSFLSSK WNGSYIVNHP FFLPGMLGIV
AAVPFGFVLA NIFV
//
