ID A0A1Q9JR07_9FIRM Unreviewed; 672 AA.
AC A0A1Q9JR07;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN ORFNames=BHF70_02635 {ECO:0000313|EMBL:OLR58617.1};
OS Anaerostipes sp. 494a.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC Anaerostipes.
OX NCBI_TaxID=1261636 {ECO:0000313|EMBL:OLR58617.1, ECO:0000313|Proteomes:UP000242190};
RN [1] {ECO:0000313|EMBL:OLR58617.1, ECO:0000313|Proteomes:UP000242190}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=494a {ECO:0000313|EMBL:OLR58617.1,
RC ECO:0000313|Proteomes:UP000242190};
RX PubMed=27613689;
RA Trachsel J., Bayles D.O., Looft T., Levine U.Y., Allen H.K.;
RT "Function and Phylogeny of Bacterial Butyryl Coenzyme A:Acetate
RT Transferases and Their Diversity in the Proximal Colon of Swine.";
RL Appl. Environ. Microbiol. 82:6788-6798(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLR58617.1}.
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DR EMBL; MJIF01000001; OLR58617.1; -; Genomic_DNA.
DR RefSeq; WP_075758803.1; NZ_MJIF01000001.1.
DR AlphaFoldDB; A0A1Q9JR07; -.
DR STRING; 1261636.BHF70_02635; -.
DR Proteomes; UP000242190; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:OLR58617.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000242190};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 16..149
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
SQ SEQUENCE 672 AA; 76485 MW; BCEF02B71B6CFD40 CRC64;
MNRICRNFSK AGIVLKSLFI TEKPSVAREF AAALNINAQN KDGYMEGEKA VITWCVGHLV
TMSYPDKYDP ALKKWDLETI PFIPKEFKYE IIPSVKKQFD IVSMLLNRKD IDTIYVCTDS
GREGEYIYRL VEQEAKVENK IRKRVWIDSQ TKEEVIRGVR EAKDLSEYDN LASAAYLRAK
EDYLMGINFS RVLTLKYGWT LGNYLNQKKS LVVAVGRVMT CVLGMIVKRE REIRNFVPTS
FYRVIAHLNC QGFEVEAEWK AVKGSRYYES SKLYKDNGFL DKKEARDFIE FLEDGSNGET
IVKSSTRRTE KKNPPLLYNL AELQNQCSKM FKLSPDETLK IVQELYERKL VTYPRTDARV
LSTAVSKEIY KNIKGLTGYA PLSTYASEIL EQKIYQGISR TKYVDDKKIT DHYAIIPTGQ
AISGLGSISQ LGQKVYDVIV RRFLSIFMPP AVYTKIKLET ETKGETFFAN FKILKDPGYL
KVAGTGNSKK EDGLTQEQMD AITSLKKGGK LPVKSYKIKN GTTTPPKRYN SGSLILAMEN
AGQLIEDEDL REQIKGSGIG TSATRAEIVK KLITNKYIAL NKKTQIVTPT LTGEMIVNVV
SASIGSLLNP ELTASWEKGL TYVAEGTVTE EEYMEKLDRF VSQKTNIVKQ NNYQYQLRQS
FDQIAPYYKK NK
//
