ID A0A1Q9LMC1_9PSEU Unreviewed; 4186 AA.
AC A0A1Q9LMC1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=Polyketide synthase {ECO:0008006|Google:ProtNLM};
DE Flags: Fragment;
GN ORFNames=BJP25_16735 {ECO:0000313|EMBL:OLR93149.1};
OS Actinokineospora bangkokensis.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Actinokineospora.
OX NCBI_TaxID=1193682 {ECO:0000313|EMBL:OLR93149.1, ECO:0000313|Proteomes:UP000186040};
RN [1] {ECO:0000313|EMBL:OLR93149.1, ECO:0000313|Proteomes:UP000186040}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=44EHW {ECO:0000313|EMBL:OLR93149.1,
RC ECO:0000313|Proteomes:UP000186040};
RA Greule A., Intra B., Flemming S., Rommel M.G., Panbangred W., Bechthold A.;
RT "The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWT reveals
RT the biosynthetic pathway of antifungal compounds Thailandins with unusual
RT extender unit butylmalonyl-CoA.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000256|ARBA:ARBA00004792}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLR93149.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MKQR01000011; OLR93149.1; -; Genomic_DNA.
DR RefSeq; WP_075974851.1; NZ_MKQR01000011.1.
DR STRING; 1193682.BJP25_16735; -.
DR OrthoDB; 9778690at2; -.
DR Proteomes; UP000186040; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt.
DR CDD; cd08952; KR_1_SDR_x; 1.
DR CDD; cd08956; KR_3_FAS_SDR_x; 1.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 1.10.287.1960; -; 1.
DR Gene3D; 3.30.70.3290; -; 3.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.30.70.250; Malonyl-CoA ACP transacylase, ACP-binding; 1.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 3.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR015083; Polyketide_synth_docking.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 3.
DR Pfam; PF08990; Docking; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 3.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 2.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 3.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 3.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 2.
DR SMART; SM01294; PKS_PP_betabranch; 2.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 5.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 3.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 3.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000186040};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 33..455
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1310..1385
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1403..1827
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 2925..3000
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3015..3433
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT REGION 463..491
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2362..2384
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2368..2384
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 4186
FT /evidence="ECO:0000313|EMBL:OLR93149.1"
SQ SEQUENCE 4186 AA; 431006 MW; FC0410275452499E CRC64;
MADDAKVLDY LKRATADLRE TRRRLAEAEG KRHEPIAVVG MSCQYPGGVR SPEDLWRLVS
EGRDGIGGFP TDRGWAEDAL RDVDGSGALT VAQGGFLADA GLFDAGFFGI SPREALAMDP
QQRLLLEHAW TAVERAGIDP RSLRGTRTGV FAGVMYHDYA VRLHGAADDV AGYVGTGSAA
SVVSGRVAYA MGLQGPTLTV DTACSSSLVA LHLAASALRQ DECQFALAGG VTVMSNAGSF
VEFSRAGGLS ADGRCKSFAE GADGTGFGEG VGLLLLEKLS DAQRNGHRVL AVLRGSAVNS
DGASSGLTAP NGASQQRVIR QALANARLET TDIDLVEAHG TGTPLGDPIE AQAVIATYGQ
DREEPLYLGS IKSNIGHTQA AAGVSGVIKA ILALRHGVMP RSLHLGTPTS QVDWESGAVS
LLTEARDWPE VDRPRRAAVS SFGISGTNAH IIVEQAVEQA VEQAVEAGTA DPDGTGDTGS
PAPAAGPTAW PLAGRTEDAL RAQAAQLKAA LVAAPDWVAA DIGHSLATTR TAFEHRAVVV
GSTREELLAG LDAITPASVT RGRTAFAFTG QGSQRAGMGR GLAAAFPVFA AAWDEVVALF
PAPVRHVLVE DQSRLDDTEF AQPAIFAFEV ALVRLFASWG VRPDVVIGHS VGEIAAAHVA
GVFTLADAAA LVVERGRLMG AVTARGAMAV IGLPEREIAL PAGVEVAAVN SADSTVVSGD
ADAVAALADE HAARGVRVKR LAVSHAFHSA HMDEAVAPLA ALVRSLPRSA PAIEFVPAAG
PGDPADADYW TRNLRGAVRF ADGAARLDAA RVVEIGPDAA LAPLLAACAP AQRRDRDEAL
TALRALGHVH ATGGEVDWSA LHTGGRVVDL PTYPFQRERY WPATEAPTST PWHYRDHWTP
VPENPPVALG SWLVVGDPDS PLAADLAARG ARVVDAAEVP GAAGSAEGVL ALHPTAASLL
ALLQSLVDTP VPVWAVTTGA LADAPEQAAV WGLGRTAALE IPERWGGLVD APADTTADRL
ALVLGGGETE VLLTPDGNRA RRLARAETAA SWTPRGTVLI TGGTGALGVR VAHWAAATAD
RLVLTSRRGG AVPDGLPEGT AVVAVDAADR EAMAALLADV EPDAVIHAAG VLDDGVLDRL
TPERLASVAA AKPVAVRVLD ELTRDRDLDA FVVFSSAAAT FGAPGQGNYA AANAELDALV
RARHAAGLPG TSIAWGPWAE DGMATGDKAA ARTARSGLAA LTADRAVTAL ALAVGSGLPA
LTVVDIDWSV FALPGRLLSD LAPARAAAPA PDLGAQAERF RALPADRRGQ ALLDLVRETA
AAVLGFASAR AVPADRAFRE LGFDSLTAVE LRTALNAATG LSLPATAVFD HPTAADLARH
VDGLLDGVAA TRVPSVVAAA GSDEPIAIVG MACRFPGGVA APEDLWDLLV AGRDGLGPFP
DDRNWDLAAL FDPDPDNPGT SYTATGGFLR DVPGFDADFF GISPREALAM DPQQRLLLET
TWEAFERAGI AAPTLRGSAT GVFVGTNGQD YAPLFTGSTE GVEGHLATGN SASVVSGRLS
YVFGLEGPAV TVDTACSASL VALHLAVQAL RSGECTLALA GGVTVMSTPT AFIEFSRQRG
LSGDGRCKAF SADADGTGWG EGVGMLVVER LSDARRHGHR VLALVRGSAV NQDGASNGLT
APNGPSQERV IRQALANARL EPSDVDMLEA HGTGTSLGDP IEAQALLATY GQGRSVPLYL
GSVKSNIGHT QAAAGVAGII KSVLALNHRT LPKTLHVDAP TPHVDWTAGS VELLTEARPW
PEADRPGRAA VSSFGVSGTN AHVILEQGDP APELAPDTAL TPLVLTARSA AALRAQAQRL
ADALPSLPAT RAAFALATTR AHLGHRAVAL GVDAVRALAA GEQHPALVQG TAGEPGRVVF
VFPGQGSQWV GMARELAASS EVFAARLAEC DAALSEFKDW SLDEALGDAA LLERVDVVQP
VLWAVMVSLA EVWRSWGVEP AAVVGHSQGE IAAAVVAGAL SLEDGARVVA LRSQVLRRLA
GRGGMVSVAL GETEVRARIA PFGDRVSVAA VNGPAAVVVS GEPAALDELV AACEADGVRA
KRVPVDYASH SAQVDELREE LLAALAPVRP RVGLVPVHST LTGRVEDGSG MDAGYWFDNL
RSTVEFAAAV TRLVDDGFGT FIECSPHPVL TMALPDDVVA VGSLKRDHGG LDRMLLSLGE
AVVAGITPDW VLVLPGAEPV DLPTYPFQHE RFWPQRVTAA AGAPSGFGQG AAGHPFLGAL
VSLADGDGAV LTGRLALDDH PWLADHVVAG RLIVPGTALV ELALCAGEHT GAPHVEELTL
HQPLTLPEQG ALRLQVSVGA DQGGRRPVSV HSRPDADDAP WTRHADGHLT ATAPEAPAVT
EWPAPGAVEV DIDGLYADLA TRDLGYGPAF RGLRRVWRDG DDVLAEVRLD DAEHAAAQRF
GLHPAVFDAA LHAIALGGFV DAGVRAALPF SFSDVRLSAT GATALRVRVS AAGPNAVTVT
AADADGAPVV RVGALALRPL DTAALAPATG AALYALDRVE VAHEPADAVL DIDGMSLADV
LAAVQAHLAD EAAGLLAVRT TDTVDGAAAR GLVRTAANEN PGRLALVDTD GSLGATPVNA
DEPELVVRDG RVLAPRLVRA ETTAVTEPFT AESRVLITGG TGTLGLLVAR HLVAEHGVRH
VVLASRSGGA VPDIDADVQV VACDVSDRDA VAALLAEHPV TAIVHAAGVL DDGVVTALTP
DRLDALLAPK LTAARHLAEL AGELTAFVLF SSAAGVFGNP GQSGYAAANS ALDALAVELR
AAGVPATSLP WGLWASASGM TGHLDGTQLR RTARGGARAL TDDDALALFS TAVRTGAAVL
APLDLDLAAV RAAGEVPPLL RALVPARRRR AAAATAAPTA DLTPAAALDL VRATTAAVLG
HASAASVDPD RAFADLGFDS LTAVELRNRL AAATGLRLPA TLVFDHPTPA ALAARVVGEA
PARRATTTAP ARPVDEPIAI VSMACRYPGG IATPEDLWEL VAGNRDVIGP FPGDRGWDVA
ALTGGRPGAP AGAFLHDAAD FDAGLFGISP REALAMDPQQ RILLEVSWET FERAGLDPHG
LRGSRTGVFA GVMYQDYASR LVAVPEGVEG HLGTGNSASV VSGRVAYAFG LEGPAITVDT
ACSSSLVAVH LAAQALRTGE CDLALAGGVT VMSTPGVFVE FTRQQGLSGD GRCRSFAEGA
NGTGFAEGAG LVLLERLSDA RRNGHHVLAV VRGSAVNSDG ASNGLTAPNG PSQQRVISAA
LTAARLDPAE VDLVEAHGTG TVLGDPIEAQ ALMAAYGQDR EQPLYLGSVK SNLGHTQAAA
GVAGIIKAVQ AMRHGVLPAS LHVDAPSGRI DWSEGAVELL TANRDWPAVD RPRRAGVSSF
GISGTNAHVV LEQVTTAPAP AVETGGPHAW VLSAHDDAAL TAQAARLAAH LTDEPAAEVA
AALATRADLP HRAVVVGDRA ELVARLATLT GPGVFRGTAA RGRTAFVFTG QGAQRVGMGR
GLAAAHPEFA RAWDAVLALY PEHVRAELAG EQTRITETEF AQPALFAFEV ALARLLESWG
VTPDVVIGHS VGEIAAAHVA GVLTLADAAR LVVARGALMG SVAARGAMAA IGAAPEDIDL
PAGVEVAAVN GPQSTVVTGD EDAVLAVLDA ARARGLKATS LEVSHAFHSA HMDEVLPELR
ALLDTVELAE PRIDFIRVAG ERTGADYWVD NVRGTVRFAD GVSRLNAAHV LEVGPDAALT
PLVAGCVPAQ RRDRDEAAAL LEALARLHVA GRTVDWARLT PTTRPLTLPT YAFQRTRYWL
DAAPTPVDPW LYRVEWDGIT LGETAAEPVV ALGDAPAPLR RITDLDELAD ETLLLVDPDP
ALLVEALGRA RTTWVVSADP AVLAVARVAA LEQPARFGGA VTITDPAGWA ALPRAIAGRE
DQLALGEHAR ARRIAPASPP RAWRPRGTVL ITGGGGALGA EAARWVAAEG ADRVVLVSRR
GADEALLAQL RATGSVEAVT VAADVTDRAA MAEVVARYAP TAVIHTAGVA HTAPLAATTA
EDYARVAAAK VEGARVLHEV VGEVDAFIVY SSIAGVWGSG EQAAYAAANA ALEALVAERR
AAGLPATAIA WGPWAGEGMA GGRAGADLAR SGLRPMEPRR AVEALA
//
