ID A0A1Q9LN32_9PSEU Unreviewed; 3164 AA.
AC A0A1Q9LN32;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Polyketide synthase {ECO:0008006|Google:ProtNLM};
DE Flags: Fragment;
GN ORFNames=BJP25_16720 {ECO:0000313|EMBL:OLR93409.1};
OS Actinokineospora bangkokensis.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Actinokineospora.
OX NCBI_TaxID=1193682 {ECO:0000313|EMBL:OLR93409.1, ECO:0000313|Proteomes:UP000186040};
RN [1] {ECO:0000313|EMBL:OLR93409.1, ECO:0000313|Proteomes:UP000186040}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=44EHW {ECO:0000313|EMBL:OLR93409.1,
RC ECO:0000313|Proteomes:UP000186040};
RA Greule A., Intra B., Flemming S., Rommel M.G., Panbangred W., Bechthold A.;
RT "The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWT reveals
RT the biosynthetic pathway of antifungal compounds Thailandins with unusual
RT extender unit butylmalonyl-CoA.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Antibiotic biosynthesis. {ECO:0000256|ARBA:ARBA00004792}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLR93409.1}.
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DR EMBL; MKQR01000010; OLR93409.1; -; Genomic_DNA.
DR STRING; 1193682.BJP25_16720; -.
DR Proteomes; UP000186040; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0033068; P:macrolide biosynthetic process; IEA:UniProt.
DR CDD; cd08952; KR_1_SDR_x; 2.
DR CDD; cd00833; PKS; 2.
DR Gene3D; 3.30.70.3290; -; 2.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 6.10.140.1830; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 4.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 2.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 2.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR041618; PKS_DE.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR015083; Polyketide_synth_docking.
DR InterPro; IPR036299; Polyketide_synth_docking_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 2.
DR Pfam; PF08990; Docking; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF08659; KR; 2.
DR Pfam; PF18369; PKS_DE; 2.
DR Pfam; PF00550; PP-binding; 4.
DR SMART; SM00827; PKS_AT; 2.
DR SMART; SM00822; PKS_KR; 2.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 4.
DR SMART; SM01294; PKS_PP_betabranch; 4.
DR SUPFAM; SSF47336; ACP-like; 4.
DR SUPFAM; SSF101173; Docking domain B of the erythromycin polyketide synthase (DEBS); 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 2.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 4.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 2.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS50075; CARRIER; 4.
DR PROSITE; PS00606; KS3_1; 2.
DR PROSITE; PS52004; KS3_2; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000186040};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 33..449
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1418..1493
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1502..1577
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1591..1666
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1684..2104
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3077..3152
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT NON_TER 3164
FT /evidence="ECO:0000313|EMBL:OLR93409.1"
SQ SEQUENCE 3164 AA; 327026 MW; 5FF984D5B7905516 CRC64;
MTTEQKLREY LKRVTGELTQ TRERLRRAEE AAGEPIAVVA AACRYPGGVA SPADLWRLVA
DGSDAVGPFP DDRGWQLGDT PFAAEGGFLY DAGRFDPGFF GISPREALVM DPQQRLLLET
SWEALERGGV DPHALRGSAT GVFVGVMYSD YASQLHRMPE GAEAYLSTSA SSSVASGRIA
YTLGLEGPAL TIDTACSSSL VAMHLAAHAL RTGECTMALA GGVTVMSTPS TFFAFNSQRG
LAGDGRCKAF ADAADGTGWS EGVGVLLLEK LSDARANGHP VLAVLRGSAV NSDGASSGLT
APNGPSQQRV ILSALRSAGL GPADVDVVEA HGTGTTLGDP IEAQALLATY GQDRSGGDPL
WLGSVKSNLG HTQAAAGVAG VIKVIEAMRH GVLPPTLHVD APTSQVDWTA GSVELLTESR
PWPEVDRPRR AAVSSFGVSG TNAHLILEQG DPVAAPAPDA GSGPVPLVLS ARSREALRAQ
AGQIAEVLPA LPLRSAALSL ATNRALLDHR AVALSADAVR ALAADESHPL LVRGTGGETG
KTVFVFPGQG SQWVGMAREL AASSEVFAAR LAECDAALSE FKDWSLDEAL GDAALLERVD
VVQPVLWAVM VSLAAVWRSW GVEPAAVVGH SQGEIAAAVV AGALSLEDGA RVVALRSKVL
RRLAGKGGMV SVAAGVEAVR GRIAGFGERL SVAAINGDSA VVVSGEPSAL DELVAGCEAD
GVRAKRIPVD YASHSAQVDE LRDELAEVLA PVRPRAGSVP VYSTLTGRVE DGSVMGAGYW
FDNLRSTVEF AGAVERLAED GFGTFVECSP HPVLTMALPE GVLAVGSLKR DDGGLSRMLL
SLGELVVRGL RPDWTALLPG ATRVDLPTYP FQRAHYWPEP AEPVEPAADP ADEEFWRAVT
AHDADALARV LDLDPAAPLS EVAPALLALR RRGRDAAAIA GWRYQVQWRP VVAPPAAPTG
TWLLITPEWP DPVFAAVADA LTARGATVVT EEVDAGAADR ASLTELVARH GHLDGVLSAL
AVDEGPHPEH PSAVAGHTAT LALLQALGDA GSTAPLWCLT QDAVTTDTGA APEQALVWGL
GRVAAQEYPE RWGGLLDLPA ELDDRTATLL ATALAGGTGE DQLALRPGSL LGRRITRAAV
PEGEGWRPRG TVLVTGGTGG LGAQTARWLA TAGAEHLVLL SRRGLDAPGA AELAAELAAT
GVRVDVTACD VTDRAALAAV VDGLELTAVV HTAAVLDDGV LDSITPERIA RVLAVKRDAT
RHLHELTLGR DLDAFVLFSS MSGTFGASGQ GNYAPGNAFL DAFAHHRRAI GLPATALAWG
PWAGGGMADG PVGELARRHG LPAMDPELAV RAMATAVGAD TAVALADIDW ERFFVAMTAT
RTSHVLREVP EVRRLLEVQA PAPAAPAAPV ARRASSREAL LDVVRSAVAA ILGYTGPEAV
DAERSFHDLG FDSVTAVELR NRITGATELK LPVTLVFDHP TPAALADHLA EQGGAVVEAS
ADDLLDLVRS CVAPILGYAG ADEVDPDRSF HDLGFDSVTA VELRNRITGL TELKLPVTLV
FDHPTPRALA EHLAEQGGAA LPAAPRESAA EEMLRLVRSC VAPVLGYATA DDVDPDRSFH
DLGFDSVTAV ELRNRITGLT ELKLPVTLVF DHPTPRALAE HLAAQVTPVT TAPVAAAGPR
AVDDDPIAIV AMACRFPGGV RTPEQFWALL DGRGDAIGPF PADRGWDLDH VYDLAGADAT
RTFLGGFLDD VADFDPGFFG ISPREALAMD PQQRLLLQTA WEAIERGGLD PKSLRGTPVG
VFAGTNGQDY TSLLLSRPAN LEGQIATGNT ASVLSGRVSY TLGFEGPAVT VDTACSSSLV
ALHLAAQALR NGECDLALAG GVTTMSTPGL FTEFALQGGL AQDGRCKAFG DSADGTGFAE
GAGLLLVERL SDARRNGHPV LAVLRGSAVN QDGASNGLTA PNGPAQQRVI RAALADARLA
PADVAFVEAH GTGTTLGDPI EAQAVLAAYG QDRAEPLWLG SVKSNIGHTQ AAAGVAGVIK
AVLALGRDRI PATLHAAEPS SHVDWSAGAV RVASTAVPWP AGPRRAGVSS FGISGTNAHV
ILEAGEPQPA AAPRVDPPAV LVPLSARTPA ALRAQAQRLS EVTAEPLDLA WSLARTRSAF
EHRAAVVGRT PQEVAAGLAE VAAGSPAAVT GTAAPTGRTA FVFPGQGAQW AGMAGALAAE
SPVFATRLAE CAAALAPHVD WVLTDVLADA DALGRVDVVQ PALWAVMVSL AELWRSWGIE
PAAVVGHSQG EIAAAVVAGA LSLEDGALVV ARRSQVLREL SGLGAMASVA LPEARVREAI
ADLGDRVGVA AVNGPAAVVI SGEPGPVDEL VAGWTRDGVR AKVLPVDYAS HSAQVERLRD
ELARVLAPVA PRAGRVPLYS TLTGQVEDGT ALDAAYWIAN LRATVDFAGA TARLAADGFG
VFVECSPHPS LTMAVQEQAE PTGPVVAVGS LRRDDGGLDR ALLSLGELVV RGVVPAWDRL
LDGGRRVDLP TYPFQQERYW PDAVAAHPAP APAAAGDAEF WAAVESGALA DVLALAPEAP
LRDALPALSA WRRERDRAAT SDAWRLRETW RPLAPRPATL SGTWLVLGAD GAWGEAVTDS
LRAHGATPVL VACDPGADRA AVAAAVRAVD APDPVGVVSL LALAHRGDPA GPVAATLAAV
QGLADAGVEA PLWALTRDAV AVGRADAVSP EQHAVWGFGR AVALEDPQRW GGLVDVPAAP
DGRLGTHLAR VLAGGRGEDQ VALRSSGAFG RRLERVAPAP AGTADWTGDG TVVITGGVGG
LGAALARHLV ARGARDLLLL SRTADTAVDL LAELRSSGAT VDATACDVTD RDQLAAALDG
RRVTAVVHAA GVLDDGVVDA ITPERLDRVL RVKAEGAIAL HELTRGADLA AFVLFSSAAG
ALGSAGQSAY AAANAHLDAL AAHRAALGLP ATSVAWGAWG GTGLAADGAV RENRLRGNGF
RPMDPADALA ALDRVIATGG PVEVVADVDW AALLPGLRAL RPTELFAELP EAAAVPEAAA
PEQTEGLAAV AAADRPRVAL ELVRTRAAAV LRHPDTDAVE PGKSFRDLGF DSLTAVEFRN
ALAAATGVRL PVTAVFDHPT PLALVDRLLA ELAGAPRGRR PPDH
//
