ID   A0A1Q9LSC6_9PSEU        Unreviewed;       510 AA.
AC   A0A1Q9LSC6;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   RecName: Full=Dioxygenase {ECO:0000256|RuleBase:RU364048};
DE            EC=1.13.11.- {ECO:0000256|RuleBase:RU364048};
GN   ORFNames=BJP25_08010 {ECO:0000313|EMBL:OLR94913.1};
OS   Actinokineospora bangkokensis.
OC   Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae; Actinokineospora.
OX   NCBI_TaxID=1193682 {ECO:0000313|EMBL:OLR94913.1, ECO:0000313|Proteomes:UP000186040};
RN   [1] {ECO:0000313|EMBL:OLR94913.1, ECO:0000313|Proteomes:UP000186040}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=44EHW {ECO:0000313|EMBL:OLR94913.1,
RC   ECO:0000313|Proteomes:UP000186040};
RA   Greule A., Intra B., Flemming S., Rommel M.G., Panbangred W., Bechthold A.;
RT   "The Draft Genome Sequence of Actinokineospora bangkokensis 44EHWT reveals
RT   the biosynthetic pathway of antifungal compounds Thailandins with unusual
RT   extender unit butylmalonyl-CoA.";
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000256|PIRSR:PIRSR604294-1,
CC         ECO:0000256|RuleBase:RU364048};
CC       Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1,
CC       ECO:0000256|RuleBase:RU364048};
CC   -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC       {ECO:0000256|ARBA:ARBA00006787, ECO:0000256|RuleBase:RU364048}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OLR94913.1}.
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DR   EMBL; MKQR01000005; OLR94913.1; -; Genomic_DNA.
DR   RefSeq; WP_075973143.1; NZ_MKQR01000005.1.
DR   AlphaFoldDB; A0A1Q9LSC6; -.
DR   STRING; 1193682.BJP25_08010; -.
DR   OrthoDB; 6636843at2; -.
DR   Proteomes; UP000186040; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR   InterPro; IPR004294; Carotenoid_Oase.
DR   PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR   PANTHER; PTHR10543:SF89; CAROTENOID 9,10(9',10')-CLEAVAGE DIOXYGENASE 1; 1.
DR   Pfam; PF03055; RPE65; 1.
PE   3: Inferred from homology;
KW   Dioxygenase {ECO:0000256|RuleBase:RU364048};
KW   Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW   ECO:0000256|PIRSR:PIRSR604294-1};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000256|RuleBase:RU364048};
KW   Reference proteome {ECO:0000313|Proteomes:UP000186040}.
FT   REGION          477..510
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         184
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         231
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         295
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT   BINDING         480
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ   SEQUENCE   510 AA;  56506 MW;  39B4A7A2AEC4E459 CRC64;
     MDVEVLGRVL TTLPEDDDHP YRTGPWRPQS VERAVADCEV VGEIPADLDG VYLRNTENPV
     HPALTGYHPF DGDGMVHVVG FRDGKAFYRN RFVRTEGFLA EQDAGKSLWA GLAEPPEVAL
     REDGWGARGR MKDASSTDVV VHAGVALTSF YQCGDLYRLD PLTLETLGRS DWQGRFPAEG
     VSAHPKVDPS GDLLFFNYGK QAPYLHYGVL DQDDRLVHYT GVELPGPRLP HDMAFTENHV
     VLNDPPLFWK PELLAQGAHV ARFHPDVPLR LGVLPKRGDG AEVRWFEAEP TFVLHFTNAY
     EDGDEIVVEG FHQGDPEGRG GHFPGDPAYA RMARMLSLDG MQTRLHRWRL NLVTGRVTEQ
     RQTDSITEFG MVNPRYAGRG HRYTYASTGE PGWFLFNGFV KHDLLTGAEE HHRYGDGVFG
     SETAVAPRVG ARAEDDAYLI TMTTDMREDR SECLVFDTAR ISDGPVARVR LPERVSSGTH
     STWAAGDELP GWRTAEDPAA ALGLGGPTPP
//