ID A0A1Q9UQT9_9PSEU Unreviewed; 437 AA.
AC A0A1Q9UQT9;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369, ECO:0000256|RuleBase:RU003370};
GN ORFNames=BJF85_06230 {ECO:0000313|EMBL:OLT40063.1};
OS Saccharomonospora sp. CUA-673.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharomonospora.
OX NCBI_TaxID=1904969 {ECO:0000313|EMBL:OLT40063.1, ECO:0000313|Proteomes:UP000186761};
RN [1] {ECO:0000313|EMBL:OLT40063.1, ECO:0000313|Proteomes:UP000186761}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CUA-673 {ECO:0000313|EMBL:OLT40063.1,
RC ECO:0000313|Proteomes:UP000186761};
RX PubMed=27902408; DOI=10.1099/mic.0.000386;
RA Schorn M.A., Alanjary M.M., Aguinaldo K., Korobeynikov A., Podell S.,
RA Patin N., Lincecum T., Jensen P.R., Ziemert N., Moore B.S.;
RT "Sequencing rare marine actinomycete genomes reveals high density of unique
RT natural product biosynthetic gene clusters.";
RL Microbiology 162:2075-2086(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000308,
CC ECO:0000256|RuleBase:RU003370};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2. {ECO:0000256|ARBA:ARBA00004751,
CC ECO:0000256|RuleBase:RU003370}.
CC -!- SIMILARITY: Belongs to the citrate synthase family.
CC {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC ECO:0000256|RuleBase:RU003406}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLT40063.1}.
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DR EMBL; MKKE01000066; OLT40063.1; -; Genomic_DNA.
DR RefSeq; WP_075850106.1; NZ_MKKE01000066.1.
DR AlphaFoldDB; A0A1Q9UQT9; -.
DR STRING; 1904969.BJF85_06230; -.
DR OrthoDB; 9800864at2; -.
DR UniPathway; UPA00223; UER00717.
DR Proteomes; UP000186761; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd06114; EcCS_like; 1.
DR Gene3D; 2.20.28.60; -; 1.
DR Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR InterPro; IPR010953; Citrate_synthase_typ-I.
DR NCBIfam; TIGR01798; cit_synth_I; 1.
DR PANTHER; PTHR42871; CITRATE SYNTHASE; 1.
DR PANTHER; PTHR42871:SF1; CITRATE SYNTHASE; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; Citrate synthase; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000186761};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW ECO:0000256|RuleBase:RU003370}.
FT ACT_SITE 315
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT ACT_SITE 372
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ SEQUENCE 437 AA; 48620 MW; 73957CC9DD0CFEB7 CRC64;
MSDATAAQSE GEKAVLRLPD GEHDFKVVRP AEGAPGIQLG KLLAQTGYIT YDPGFVNTGA
ASSAITYIDG DAGILRYRGY PIEQLAEQSS FVEVSYLLMN GELPNQQQLD SFTAKINRHT
LLHEDLKRFF DGFPRDAHPM PVLSSAVSAL STFYQDALDP FDKNNVELST IRLLAKVPTI
AAYAYKKSIG QPFLYPDNSL GLVENFLRMT FGLPAEPYEI DPVMAKALDQ LFILHADHEL
NCSTSTVRLV GSSEANMFAS ISAGIMALFG PLHGGANSAV LDMLENIKAD GGDVDKFVER
VKNKEAGVRL MGFGHRVYKN YDPRAKIIKK TADEILQKIG GNDELLDIAK RLEERALSDD
YFVERKLYPN VDFYTGLIYR AMGFPTQFFT VLFALGRLPG WIAHWREMIE DPETKIGRPR
QIYTGEKARD YKPLSAR
//
