ID A0A1Q9USL0_9PSEU Unreviewed; 801 AA.
AC A0A1Q9USL0;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Replicative DNA helicase {ECO:0000256|ARBA:ARBA00021957, ECO:0000256|RuleBase:RU362085};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU362085};
GN ORFNames=BJF85_06135 {ECO:0000313|EMBL:OLT40695.1};
OS Saccharomonospora sp. CUA-673.
OC Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC Pseudonocardiaceae; Saccharomonospora.
OX NCBI_TaxID=1904969 {ECO:0000313|EMBL:OLT40695.1, ECO:0000313|Proteomes:UP000186761};
RN [1] {ECO:0000313|EMBL:OLT40695.1, ECO:0000313|Proteomes:UP000186761}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CUA-673 {ECO:0000313|EMBL:OLT40695.1,
RC ECO:0000313|Proteomes:UP000186761};
RX PubMed=27902408; DOI=10.1099/mic.0.000386;
RA Schorn M.A., Alanjary M.M., Aguinaldo K., Korobeynikov A., Podell S.,
RA Patin N., Lincecum T., Jensen P.R., Ziemert N., Moore B.S.;
RT "Sequencing rare marine actinomycete genomes reveals high density of unique
RT natural product biosynthetic gene clusters.";
RL Microbiology 162:2075-2086(2016).
CC -!- FUNCTION: Participates in initiation and elongation during chromosome
CC replication; it exhibits DNA-dependent ATPase activity.
CC {ECO:0000256|ARBA:ARBA00002835, ECO:0000256|RuleBase:RU362085}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU362085};
CC -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC {ECO:0000256|ARBA:ARBA00008428, ECO:0000256|RuleBase:RU362085}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OLT40695.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MKKE01000055; OLT40695.1; -; Genomic_DNA.
DR RefSeq; WP_075849845.1; NZ_MKKE01000055.1.
DR AlphaFoldDB; A0A1Q9USL0; -.
DR STRING; 1904969.BJF85_06135; -.
DR OrthoDB; 9773982at2; -.
DR Proteomes; UP000186761; Unassembled WGS sequence.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00984; DnaB_C; 1.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR InterPro; IPR007692; DNA_helicase_DnaB.
DR InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR00665; DnaB; 1.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR PANTHER; PTHR30153:SF2; REPLICATIVE DNA HELICASE; 1.
DR PANTHER; PTHR30153; REPLICATIVE DNA HELICASE DNAB; 1.
DR Pfam; PF00772; DnaB; 1.
DR Pfam; PF03796; DnaB_C; 2.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF48024; N-terminal domain of DnaB helicase; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS51199; SF4_HELICASE; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU362085};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU362085};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU362085};
KW Helicase {ECO:0000256|RuleBase:RU362085, ECO:0000313|EMBL:OLT40695.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362085};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU362085}; Primosome {ECO:0000256|RuleBase:RU362085};
KW Reference proteome {ECO:0000313|Proteomes:UP000186761};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 196..233
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
FT DOMAIN 358..484
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 575..801
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 801 AA; 88253 MW; ED39C4F1C23FFE30 CRC64;
MAITDDRGTD VAGGEHGTFE RQPPQDSKAE QSVLGGMLLS KDAIADVVEV LRPGDFYLPK
HQAVYDCILD LYGRGEPADP ITVSAELERR GELARVGGAP YLHTLIATVP TAANAGFYAQ
IVAEKAVLRR LVEAGTRIVQ YGYGADNNDG GDINEVVDRA QAAIYEVTER RTSEDYVALE
ELLQPTMDEI DAIASRGGDA QGIPTGFADL DEVTNGLHPG QMIIIAARPG VGKALALDTP
LPTPDGWTTM GQVQPGDRLI GSDGTPTTVV AATDVMHGRP CYEVEFSDGS TIVADAEHQW
LTDTRASRRV RGPAEVRTTA ELADTVRCDT ADGRVNHSVR LAEPLELPER DLPLHPYALG
AWLGDGHSDG ARFTTADPEI ITYLEAEADL YCPRRGTYAY SLRFPTPDLD SSPEIDPVLR
SLGVLGNKHV PAEYLRGSVY QRRALLAGLL DTDGTVGGSG GVQFSVTDHR LAADVYELIV
SLGYRCSLTT KRVRGRTEAS STAYTLNFTT HDRVFWLQRK HLLHKERMTG RRPRPTPRYI
VDVRPIDSVP VRCVEVSADD HLYLAGRSMI PTHNSTLGLD FARSCSIKHG MTSAIFSLEM
SRTEIVMRML SAEARIRLAD MRGGRMSDDD WTRLARRMSE ISEAPLFVDD SPNLTMMEIR
AKARRLKQRN DLKLVVVDYM QLMTSGKRVE SRQQEVSEFS RQLKLLAKEI EVPVIAISQL
NRGPEQRTDK RPMLSDLRES GSLEQDADIV MLIHRPDAFE QDDPRAGEAD LILGKHRAGP
TSTIAVAHQL HYSRFADLAR E
//
