ID A0A1R1A7I3_9BACL Unreviewed; 535 AA.
AC A0A1R1A7I3;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE SubName: Full=Glycoside hydrolase 43 family protein {ECO:0000313|EMBL:OME81397.1};
GN ORFNames=BK120_17230 {ECO:0000313|EMBL:OME81397.1};
OS Paenibacillus sp. FSL A5-0031.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1920420 {ECO:0000313|EMBL:OME81397.1, ECO:0000313|Proteomes:UP000187447};
RN [1] {ECO:0000313|EMBL:OME81397.1, ECO:0000313|Proteomes:UP000187447}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FSL A5-0031 {ECO:0000313|EMBL:OME81397.1,
RC ECO:0000313|Proteomes:UP000187447};
RA Beno S.M.;
RT "Paenibacillus species isolates.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OME81397.1}.
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DR EMBL; MRTD01000009; OME81397.1; -; Genomic_DNA.
DR RefSeq; WP_076268735.1; NZ_MRTD01000009.1.
DR AlphaFoldDB; A0A1R1A7I3; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000187447; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd09000; GH43_SXA-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187}.
FT DOMAIN 331..532
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 15
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 187
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 128
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 535 AA; 61326 MW; 3F2B51CBAA0AE2D2 CRC64;
MTAIQNPILP GFNPDPCICR VGEDYYIAVS TFEWFPGVGI YHSKDLKNWR LAARPLSRLS
QLNMMGNPDS GGIWAPALSY SDNKFWLIYT DVKVTEGQWK DCHNYLVTCD TIDGEWSEPI
HVNSSGFDPS LFHDEDGKKY FVNMMWDQRV GHHNFYGILL QEYSASEQKL IGKPEIIFKG
TDIKLTEAPH LYKMNGYYYL LTAEGGTKYE HQATIARSKQ LTGPYEVHPE NPLITSFHAP
RIPLQKAGHA SIVQTHTGEW FLVHLVGRPL SREGQPLLDP RGYCPLGRET AIQRLEWRND
WPYVVGGNQP SLVIEGPNIP EVQWERDYPV KDDFDSSTLN LHFQSLRIPL GDQIVSLTDN
PGHLRLYGKE SLTSKFTQSF IARRWQHFNF TAETKIAFHP TSFQQSAGLV NYYNTQNWTS
CQISWNEEKG RILQLVACDN FSFSEPLQGS EIAIPEDVAY VHIRVDVENT LYYYSYSFDG
KSWTRLPVNF ECYKLSDDYI QGGGFFTGAF VGMQCQDTSG RSLHADFDYF IYDNH
//
