ID A0A1R1BKK4_9BACL Unreviewed; 526 AA.
AC A0A1R1BKK4;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE SubName: Full=Glycoside hydrolase 43 family protein {ECO:0000313|EMBL:OMF08975.1};
GN ORFNames=BK127_27465 {ECO:0000313|EMBL:OMF08975.1};
OS Paenibacillus sp. FSL H7-0331.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1920421 {ECO:0000313|EMBL:OMF08975.1, ECO:0000313|Proteomes:UP000187480};
RN [1] {ECO:0000313|EMBL:OMF08975.1, ECO:0000313|Proteomes:UP000187480}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FSL H7-0331 {ECO:0000313|EMBL:OMF08975.1,
RC ECO:0000313|Proteomes:UP000187480};
RA Beno S.M.;
RT "Paenibacillus species isolates.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OMF08975.1}.
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DR EMBL; MRTH01000023; OMF08975.1; -; Genomic_DNA.
DR RefSeq; WP_076235041.1; NZ_MRTH01000023.1.
DR AlphaFoldDB; A0A1R1BKK4; -.
DR STRING; 1920421.BK127_27465; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000187480; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd09000; GH43_SXA-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF12; BETA-XYLOSIDASE-RELATED; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000187480}.
FT DOMAIN 323..520
FT /note="Beta-xylosidase C-terminal Concanavalin A-like"
FT /evidence="ECO:0000259|Pfam:PF17851"
FT ACT_SITE 15
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 187
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 128
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 526 AA; 59957 MW; 836120F58AD41E6F CRC64;
MQTITNPILT GFNPDPSILR VGEDYYIATS TFEWFPGVQI HHSKDLVHWR LLTRPLNRIS
QLNMEGELNS GGVWAPCLTY DDGLFYLIYT DVKSRKGAFK DTHNYMVTAP DMMGPWSEPI
FLNSSGFDPS LFHDDDGRKW LVNMLWDHRK GNNKFAGTAL QEYSVEERRL IGPVINIFKG
TELKLTEGPH LYKRNGFYYL MTAEGGTQYN HAVTMARAKS IEGPYEVDPT NPILTSAGKP
ELALQKAGHG SLVETHTGEW YLAHLCARPV KDKYCNLGRE TALQRCYWTE DHWLRVEEGN
WPKSSVKAPR IEPFVFEAEP VVDHFDDASL GVVWNTLRIA PGPDWLSLTE RPGFLRLKGM
ESMSSLHRQS LVARRQQAFH CEVETVVEFE PDNFQQLAGL IVYYDTEDYM YLRITHHEVH
GKCIGIIQSV GGVYDELLDT DIPLQDITRC KLKAIIDREA LQFYYAAEGG EWAAIGPVVD
ICHLSDDYPD YIRFTGTFIG MCVQDLSGTR KHADFDSFSY IEKAVE
//
