ID A0A1R1L743_9MICC Unreviewed; 829 AA.
AC A0A1R1L743;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Replicative DNA helicase {ECO:0000256|ARBA:ARBA00021957, ECO:0000256|RuleBase:RU362085};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551, ECO:0000256|RuleBase:RU362085};
GN ORFNames=BKD30_13175 {ECO:0000313|EMBL:OMH23365.1};
OS Tersicoccus phoenicis.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Micrococcaceae;
OC Tersicoccus.
OX NCBI_TaxID=554083 {ECO:0000313|EMBL:OMH23365.1, ECO:0000313|Proteomes:UP000187085};
RN [1] {ECO:0000313|EMBL:OMH23365.1, ECO:0000313|Proteomes:UP000187085}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1p05MA {ECO:0000313|EMBL:OMH23365.1,
RC ECO:0000313|Proteomes:UP000187085};
RA Nakajima Y., Yoshizawa S., Nakamura K., Ogura Y., Hayashi T., Kogure K.;
RT "Draft genome of Tersicoccus phoenicis 1P05MA.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Participates in initiation and elongation during chromosome
CC replication; it exhibits DNA-dependent ATPase activity.
CC {ECO:0000256|ARBA:ARBA00002835, ECO:0000256|RuleBase:RU362085}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665,
CC ECO:0000256|RuleBase:RU362085};
CC -!- SIMILARITY: Belongs to the helicase family. DnaB subfamily.
CC {ECO:0000256|ARBA:ARBA00008428, ECO:0000256|RuleBase:RU362085}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OMH23365.1}.
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DR EMBL; MRDE01000076; OMH23365.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1R1L743; -.
DR STRING; 554083.BKD30_13175; -.
DR OrthoDB; 9773982at2; -.
DR Proteomes; UP000187085; Unassembled WGS sequence.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR CDD; cd00984; DnaB_C; 1.
DR CDD; cd00081; Hint; 2.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 2.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR036185; DNA_heli_DnaB-like_N_sf.
DR InterPro; IPR007692; DNA_helicase_DnaB.
DR InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR InterPro; IPR007693; DNA_helicase_DnaB-like_N.
DR InterPro; IPR016136; DNA_helicase_N/primase_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR00665; DnaB; 1.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR PANTHER; PTHR30153:SF2; REPLICATIVE DNA HELICASE; 1.
DR PANTHER; PTHR30153; REPLICATIVE DNA HELICASE DNAB; 1.
DR Pfam; PF00772; DnaB; 1.
DR Pfam; PF03796; DnaB_C; 2.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 1.
DR SUPFAM; SSF48024; N-terminal domain of DnaB helicase; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
DR PROSITE; PS51199; SF4_HELICASE; 2.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU362085};
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU362085};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU362085};
KW Helicase {ECO:0000256|RuleBase:RU362085, ECO:0000313|EMBL:OMH23365.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362085};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU362085};
KW Primosome {ECO:0000256|ARBA:ARBA00022515, ECO:0000256|RuleBase:RU362085};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000};
KW Reference proteome {ECO:0000313|Proteomes:UP000187085};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 185..389
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
FT DOMAIN 505..599
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 746..768
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT DOMAIN 768..828
FT /note="SF4 helicase"
FT /evidence="ECO:0000259|PROSITE:PS51199"
SQ SEQUENCE 829 AA; 91179 MW; 21522CFFC3F794AF CRC64;
MDREQRSRQD EFSRTPPQDL VAEESVLGGM MLSKDAIADV VEVLKGNDFY RPAHENIYDA
IVTLYGKGEP ADAVTVADEL NKRGELSRIG GPAYLHTLIS SVPTAANAGF YAEIVRERAV
LRRLVDAGTK IVQLGYSNDG EVEDLVNAAQ AEIFSVADTR ANEDYLPLQA IMEHTIDEIE
SASGRGEGML GVPTGFYEFD ELTHGLQPGQ MIVIAARPAV GKSTFALDIA RSAAIKHELP
TVVFSLEMSR NEIAMRILSA EATINLQDLR KGSLRQEHWT KIAAVSQRLN DAPLFIDDSP
NMSLMEIRAK CRRLKQNHGL KLVVLDYLQL MSSGKRVESR QQEVSEFSRA LKLLAKELEI
PVIALSQLNR GSEQRTDKRP MVSDLRESGC LTADTRILRS DTGAETTMGE LYASGATDVP
VWSVGEDLRY VERHLTHVFS TGVKPVFRLT TASGRTLRAT ANHPFLTYDG WRALGDLTPG
TRIAAPRHTD GPTERTEWED ARVVMLAHLL GDGSFVRRQP IRYASVDEHN LAAVTVGGFV
PQAVFALPKV QIALFLKHLW STGGRVSISR DRRTGSITLP STSRRLVDDV ARLLLRFGIT
GRIRGGSAKG HKTSIRPRRQ GYVLEIAGTD DQRRFLQEIG VEGECVSAAA SLLRIIRARK
VGGCAPVEPT GIWDEVTSIL ADKQSAPRPT SLSEQLGGPT LVGVDRSVAR MGQIARVLDR
VEMDVLAVND VFWDKIISVE PDGEEEVFDA TVLGNHNFIA NGLAVHNSIE QDADMVVLLH
REDLYNKESE KAGSADIMVA KHRNGPTKNI EVAFQGHYSR FSNMAPDGM
//
