ID A0A1R3KC67_9ROSI Unreviewed; 1110 AA.
AC A0A1R3KC67;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 13-SEP-2023, entry version 25.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=COLO4_09454 {ECO:0000313|EMBL:OMP04628.1};
OS Corchorus olitorius.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Grewioideae; Apeibeae; Corchorus.
OX NCBI_TaxID=93759 {ECO:0000313|EMBL:OMP04628.1, ECO:0000313|Proteomes:UP000187203};
RN [1] {ECO:0000313|Proteomes:UP000187203}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. O-4 {ECO:0000313|Proteomes:UP000187203};
RA Alam M., Haque M.S., Islam M.S., Emdad E.M., Islam M.M., Ahmed B.,
RA Halim A., Hossen Q.M.M., Hossain M.Z., Ahmed R., Khan M.M., Islam R.,
RA Rashid M.M., Khan S.A., Rahman M.S., Alam M., Yahiya A.S., Khan M.S.,
RA Azam M.S., Haque T., Lashkar M.Z.H., Akhand A.I., Morshed G., Roy S.,
RA Uddin K.S., Rabeya T., Hossain A.S., Chowdhury A., Snigdha A.R.,
RA Mortoza M.S., Matin S.A., Hoque S.M.E., Islam M.K., Roy D.K., Haider R.,
RA Moosa M.M., Elias S.M., Hasan A.M., Jahan S., Shafiuddin M., Mahmood N.,
RA Shommy N.S.;
RT "Corchorus olitorius genome sequencing.";
RL Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OMP04628.1}.
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DR EMBL; AWUE01014234; OMP04628.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1R3KC67; -.
DR STRING; 93759.A0A1R3KC67; -.
DR OrthoDB; 656at2759; -.
DR Proteomes; UP000187203; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR GO; GO:0009791; P:post-embryonic development; IEA:UniProt.
DR GO; GO:0048608; P:reproductive structure development; IEA:UniProt.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000187203}.
FT DOMAIN 19..654
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 715..866
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1110 AA; 127220 MW; A6FD9C35B311A18E CRC64;
MEDVCEGKDF SFPSQEETIL SFWSKIDAFN VLLERTKNLP EYIFYDGPPF ATGLPHYGHI
LAGTIKDSVT RYQSRTGHHV TRRFGWDCHG LPVENEIDGK LGIKRRDEIL EMGIDKYNEE
CRSIVTRYVE EWEKIVTRTG RWIDFKNDYK TMDFKYMESV WWVFSQLYQK GLIYRGCKVM
PYSTGCKTPL SNFEAGQNYK TVHDPEIMVA FPIVGDPDNA AFVAWTTTPW SLPSNLALCV
NANFVYLKVH NKYSVKIYVV AESRLSELPT EKPNKSKTKD SAAESFEVLD KFYGASLVGK
KYEPLFNYFS EFSDTAFRVV ADNYVKDNSG TGIVHCAPAF GEDDYRVCIG NQIINKEDQN
LINVAVDEDG CFTGKITDFS RRYVKDADKD VIEAVKAKGR LVKHGTITHS YPFCWRSETP
LIHRAVPSWF VRVEQLKEQL LENNKQTHWV PDYVKDKRFH NWLENARDWA ISRSRFWGTP
IPVWISEDGE EVVVMDSVET LERLSGVKIS DLHRHNIDHI TIPSSRGPEF GVLRRIDDVF
DCWFESGSMP YAYIHYPFEN VELFEKNFPG QFVAEGLDQT RGWFYTLMVL STALFGKPAF
RNLICNGLVL AEDGKKMSKK LKNYPPPMEV INEYGADAVR LYLINSPVVR AETLRFKKEG
VYGVVKDVFL PWYNAYRFLV QNAKRLEYET SAPFVPNLMD LSKSNLDEWS SNVLDRWIYS
ATQSLVHFVR KEMDAYRLYT VVPYLLKFLD DLTNIYVRFN RKRLKGRTGD QTDCQAALST
LYNVLLTSCK VMAPFTPFFT EHLYQNLRKV LGDGAEESIH FCSFPQVEEG GRDHRIEESV
ARMMKIIDLG RNIRERHNKP LKAPLRGMVV VHPDEEFLDD IAGKLSEYVK EELNIRNLDT
CKDPLKYACL RAEPDFSVLG KRLGKSMGEV AKNVKAMSQE DILAFQEAGE VTFVTKSGAA
HCLKLTDIKV ERKFKRPDNG MKDGEIFDAA GEGDVLVVLD LRDDESLFEA GAAREIVSRI
QKLRKKAGLE LEPTDVVEVY FESLDQDKSA IQQVLNSQES YIKDAIGSSP FIISLNMMPL
HTVVLCEESF QGISNMSFKI SLARPGEETT
//
