ID   A0A1R3US65_9ACTN        Unreviewed;       835 AA.
AC   A0A1R3US65;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   27-MAR-2024, entry version 32.
DE   RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE            EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN   ORFNames=BQ8420_23305 {ECO:0000313|EMBL:SIO89776.1};
OS   Nocardiopsis sp. JB363.
OC   Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC   Nocardiopsaceae; Nocardiopsis.
OX   NCBI_TaxID=1434837 {ECO:0000313|EMBL:SIO89776.1, ECO:0000313|Proteomes:UP000195722};
RN   [1] {ECO:0000313|EMBL:SIO89776.1, ECO:0000313|Proteomes:UP000195722}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JB363 {ECO:0000313|EMBL:SIO89776.1,
RC   ECO:0000313|Proteomes:UP000195722};
RA   Song W.-J., Kurnit D.M.;
RL   Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-dependent breakage, passage and rejoining of double-
CC         stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC   -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC       family. {ECO:0000256|ARBA:ARBA00008263}.
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DR   EMBL; FRFF01000140; SIO89776.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1R3US65; -.
DR   OrthoDB; 9806486at2; -.
DR   Proteomes; UP000195722; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR   GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR   CDD; cd00187; TOP4c; 1.
DR   Gene3D; 3.30.1360.40; -; 1.
DR   Gene3D; 2.120.10.90; DNA gyrase/topoisomerase IV, subunit A, C-terminal; 1.
DR   Gene3D; 3.90.199.10; Topoisomerase II, domain 5; 1.
DR   Gene3D; 1.10.268.10; Topoisomerase, domain 3; 1.
DR   InterPro; IPR006691; GyrA/parC_rep.
DR   InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR   InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR   InterPro; IPR013758; Topo_IIA_A/C_ab.
DR   InterPro; IPR013757; Topo_IIA_A_a_sf.
DR   InterPro; IPR002205; Topo_IIA_dom_A.
DR   PANTHER; PTHR43493:SF5; DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43493; DNA GYRASE/TOPOISOMERASE SUBUNIT A; 1.
DR   Pfam; PF03989; DNA_gyraseA_C; 3.
DR   Pfam; PF00521; DNA_topoisoIV; 1.
DR   SMART; SM00434; TOP4c; 1.
DR   SUPFAM; SSF101904; GyrA/ParC C-terminal domain-like; 1.
DR   SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:SIO89776.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000195722};
KW   Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT   DOMAIN          19..474
FT                   /note="DNA topoisomerase type IIA"
FT                   /evidence="ECO:0000259|SMART:SM00434"
FT   REGION          790..809
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          446..473
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COMPBIAS        793..809
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   835 AA;  91170 MW;  C018C832872EBA99 CRC64;
     MARTTSRPPE DLAEKIIDID VSEEMRGSFL EYAYSVIYQR ALPDARDGMK PVQRRILYQM
     NEMGLRPDRG HVKCARVVGE VMGRLHPHGD SAIYDALVRL SQSFAMRVPL VDGHGNFGSL
     GGDDAPAAMR YTEARLDRPA ELLVSSIDED VVDFKPNYDG QETEPEVLPA AFPNLLVNGT
     SGIAVGMATN MAPHNLGEVI AAARHLIAHP EAGLDELMEF VPGPDLPTGG TIAGLDGVRD
     AYRKGRGTFK TRATVSIEKV SARRTGLVIT ELPYSVGPEK VVARIKELVQ SKKLQGISDL
     KDLTDRNQGL RLVIELKNGF NPEAVLEELY RLTPMEESFG INNVALVDGQ PQTLGLLELL
     QVYVGHRLEV VRRRSEFRRK KRRERLHLVD GLVVALLDID RVIALIREAD DTAAAREALM
     GAYEMSEIQA RYILDTPLRR LTRFDRLELE TEREKLNKEI DELTSILESD KKLRKLVSKE
     LNEVSKKFAT PRRTQLRESD GATRSAVIPL EMADQPCHVL FGVEGRIVRT KDGTLPRPHE
     GLRVAHDTLH VSVPTTSRSD VGLVTSLGRM IRVPVVELPE LPEPPAPLEG DQDPPPPLTH
     GLDVGEFVAL DGDETVVSVV SMRVDGPGFV VGTLGGVVKR VAPDYPPNKD DFEIITLKDD
     DRILGVTQLS TGEEDLAFVT SEAQLLRFSA STARPQGRLA GGVAGVKLAE DARVLWFGAV
     PSPEDSVVVT VSGSGNALAG TQAGSAKVTP FESYPTKGRA TGGVRCHRYL KGEDTLLFAW
     VGRSPARATR ADGKPVRLPD LTERRDGSGE ALTRAITTVG SGQIDFGISA PDQHA
//