ID A0A1R3W6F1_9GAMM Unreviewed; 1127 AA.
AC A0A1R3W6F1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=SAMN05216526_1840 {ECO:0000313|EMBL:SIT73253.1};
OS Ectothiorhodosinus mongolicus.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Chromatiales;
OC Ectothiorhodospiraceae; Ectothiorhodosinus.
OX NCBI_TaxID=233100 {ECO:0000313|EMBL:SIT73253.1, ECO:0000313|Proteomes:UP000223759};
RN [1] {ECO:0000313|EMBL:SIT73253.1, ECO:0000313|Proteomes:UP000223759}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M9 {ECO:0000313|EMBL:SIT73253.1,
RC ECO:0000313|Proteomes:UP000223759};
RA Mah S.A., Swanson W.J., Moy G.W., Vacquier V.D.;
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
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DR EMBL; FTPK01000003; SIT73253.1; -; Genomic_DNA.
DR RefSeq; WP_076756212.1; NZ_FTPK01000003.1.
DR AlphaFoldDB; A0A1R3W6F1; -.
DR STRING; 233100.SAMN05216526_1840; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000223759; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000223759}.
FT DOMAIN 591..752
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 765..927
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1127 AA; 125571 MW; 89AD1B59B4D15396 CRC64;
MTSSPAPGSA GFLAAAQQLQ SAQGVCVFIT DGAQAAERLR DALRFFLNDP QLPVLSLPDW
ETLPYDVFSP HEDIISQRLK TLYALPELKR GALVLPISTA LLRLPPRSWL ASECLNLSVG
ERLDRDTLLA QFTAAGYQSV SQVQAHGEFA VRGALLDVWP MGSEQPLRID LLDDEIETIR
LFDPQTQRSL SHIEAVRLLP AHEFPLNEAS IKRFRANYRD QLAGDPNVSV IYREVSQGQA
PSGIEYYLPL FFEQVETLFD HLPANLEIYE IGDVGSAAKQ FYEQLEARYE QRRYDPERPL
LPPEALYLNP GDYQNRAKAQ RGRLMGKKSK ALPEVQLQGR DAQSAAGLRD FLNTLKGRCL
LLAETAGRRE VLLDTLREQE LRPQVFEHWA DFIASDASLG LCIGPLESGF HLPEKELAVI
TEAELFGQKA RQERRQKKPT RDADAIVRNL TDLHPSAPVV HEEHGVGRYL GLNTLSTGGQ
VTEYLMLEYA GGDKLYVPVS ALHLISRYTG SDPEHAPLHK LGSEQWSKAR RKAAEKAHDV
AAELLEIHAR RAARQGHAHA LSEHDYQAFT EAFPFEETPD QAEAIRAVIA DMQSTQPMDR
VVCGDVGFGK TEVAMRAAFV AVQGGSQVAV LVPTTLLAQQ HEQNFRDRFA DWPVRIESLS
RFGSAKEQKV VMQGLAEGKV DIVIGTHKLI QGMLEFKNLG LVIIDEEQRF GVRHKEKLKA
LRAEVDLLTL TATPIPRTLN MALAGLRDLS IIATPPGERL AIKTFVSQDS DALVQEACWR
EMRRGGQVYF LHNEVQSIER RARQLAELMP EARIGIAHGQ MREKDLERVM LDFYHRRFNI
LVCTTIIESG IDVPTANTII MDRADKLGLS QLHQLRGRVG RSHHRAYAYL LTPHPSAMTK
DAVKRLEAIE SLGELGVGFT LASHDLEIRG AGELLGEEQS GQIQEVGFSL YNDLLERAVK
ALQSGQLPDL SRMGSDSTEV ELGLPALLPE DYVPDVHVRL ILYKRISACV DEDGLRELEV
ELIDRFGLLP PPAKHLMASA RLRQQLAPLG VRKLEMGAAG GRLIFQDQPP VDAQSVIQLL
QTAPTRYALD GPTKLRFTQP LPDLDKRIQA VADMLSILRP EPERKAA
//
