ID A0A1R4FC74_9MICO Unreviewed; 880 AA.
AC A0A1R4FC74;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=FM112_03060 {ECO:0000313|EMBL:SJM53504.1};
OS Gulosibacter sp. 10.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Gulosibacter.
OX NCBI_TaxID=1255570 {ECO:0000313|EMBL:SJM53504.1, ECO:0000313|Proteomes:UP000195931};
RN [1] {ECO:0000313|EMBL:SJM53504.1, ECO:0000313|Proteomes:UP000195931}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=10(10) {ECO:0000313|Proteomes:UP000195931};
RA Peterson S.W.;
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
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DR EMBL; FUIC01000008; SJM53504.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1R4FC74; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000195931; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:UniProt.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000195931};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 1..259
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 636..843
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 880 AA; 96652 MW; C078D65DD14813EA CRC64;
MVIDGHSLAF RAFYALPVDS FVNSEGQHTN AIHGFISMLL SLMRDENPSH VAVAFDAGSQ
SFRNEEYPEY KGGREETPPE FRGQIELLQE SLDAMGITWM QKDNFEADDI LATLASQGED
QGFDVVVVSG DRDAIQLVNE ETTLLYPSVQ GVSKLTRYTP ETVFEKYGVA PSQYPELAAM
VGEKADNLPG IPKVGPKTAA KWIAQFGSLE EILANDDKLT GKVGESFREH RENAIRNRRL
NRLLEDVELP KRPEDLVIRP IDVDAVREVF TRLQFRTLQD RVLKLATERD GAIVTADTTS
ALDGLGIKGA LDGDGLADWL GAAVSATADG LGLVLVFGEG GLVEAGVASR TEAARVVVQE
GADLAAFWEW LESDAPKVMF EAKPQLKRGI LAGVRVGGTV FDAKIAAFLV NPTGVPKRLS
ELVQQYLGET LPEADPNQLA TLEEETTDFL ASDAWFVKRA ADAAIGRLPE ATKPVFADIE
SPLTSVLAGM ETTGIAMDRE LLETLAKEQG EHVTEYENEA FDAVGGERFN LASPKQLQTV
LFEQLEMPKT RRTKTGYSTD AQSLEELELK NPHPFLGALR RHRDASKLKQ MMESLLKAIG
EDGRIHTTYL QTGAATGRLA STDPNLQNIP IRSPEGQRIR EAFVADGEYV ELLTADYSQI
EMRIMAHLSG DESLIDAFNR GEDTHRFVGS RVFGVEPGEV SAEMRTKVKA MSYGLVYGLS
AFGLSKQLGI PQSEAKELMT GYFERFGRVR DYLRESVAKA REDGYTETIF GRRRPFPDLN
SNNRVARQNA ERAALNAPIQ GSAADIIKLA MLDVAERLRQ EGLRSRMLLQ VHDELVFEVV
DGEFDALEGL VRTAMAGAAD LSVPLDVSVG RGKNWQTAGH
//
