ID   A0A1R4ITG5_9MICO        Unreviewed;      1047 AA.
AC   A0A1R4ITG5;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   24-JAN-2024, entry version 23.
DE   RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE            EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN   ORFNames=FM104_03815 {ECO:0000313|EMBL:SJN22969.1};
OS   Microbacterium esteraromaticum.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=57043 {ECO:0000313|EMBL:SJN22969.1, ECO:0000313|Proteomes:UP000196320};
RN   [1] {ECO:0000313|EMBL:SJN22969.1, ECO:0000313|Proteomes:UP000196320}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B Mb 05.01 {ECO:0000313|EMBL:SJN22969.1,
RC   ECO:0000313|Proteomes:UP000196320};
RA   Peterson S.W.;
RL   Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC         fragments with terminal 5'-phosphates, ATP is simultaneously
CC         hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC   -!- SIMILARITY: Belongs to the HsdR family.
CC       {ECO:0000256|ARBA:ARBA00008598}.
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DR   EMBL; FUKO01000012; SJN22969.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1R4ITG5; -.
DR   OrthoDB; 9758243at2; -.
DR   Proteomes; UP000196320; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.1570.50; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR   InterPro; IPR040980; SWI2_SNF2.
DR   PANTHER; PTHR42927; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR42927:SF1; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF04313; HSDR_N; 1.
DR   Pfam; PF18766; SWI2_SNF2; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:SJN22969.1};
KW   Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000196320};
KW   Restriction system {ECO:0000256|ARBA:ARBA00022747}.
FT   DOMAIN          295..528
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000259|SMART:SM00487"
SQ   SEQUENCE   1047 AA;  114949 MW;  FDD5EC1F38BD9A45 CRC64;
     MSDAQLLEHE FETNLCEELA ERGWLYEDAG RTAAAGWDVG LALVPRDVLS WLQTQYPDEY
     EKAVPSDLVN GQRDAAERGL LEHLTKELAK QTKMDPTTGH PVGGLLGVLR KGFSYAQIGR
     PAAKFGPMVI FPPANPNLIE VVEQSDAVVL RILRQVRFDT ATTESIDVVL TANGLPIVTM
     ELKTDNTQTV QHAIRQYKED RKPGRTRALL APGRALVHFA VSTDLVFMTT KLQGGDTVFL
     PFNQGNNGHE GNPVSDTGSP ANYLWRDILA RSTFLRILKD FALFEPSKTG KKDGRLVFPR
     FHQLRAVERV VGDIEQRGPG GKYLIWHSAG SGKTKTIAWL SHRLIRHMSA DSKSTFDSVI
     VVTDRTVLDE NVRDDMNLVQ SSKGLVVSVG EKSGAKSPQL KKALLEGDHI ITCTLQTFPE
     VMKLIENMDD LRGRRWAVVA DEAHSSQSGA SARQLKELLA DVVGLDFDED DIDAELSAED
     LLRAKNSAVA NAENITFIAL TATPKAKTLR LFGTQNLETD RWEAFDTYTM AQAIEEGFIL
     DVLRNYSTYD MFLRVKNAIE GEEDTEIQVN TGEAVTNIVR YARLHPTAVA QKVRVVVEHF
     RRNVAHLLGG EARAMVVTGT RMEAYTWSKK MNAYIAEQGY TDMDTLVAFS GSLADGSGDR
     VTEVSMNGVS DVAAAFREEG IYKVLIVANK FQTGFDEPRL MAMYVDKKLS GITTVQTLSR
     LNRMYPGKTS PMVVDFMNSP EHIEKDFQLY YEDAHVEGEV DPNALYTLAE RLDTAGYYTE
     SGLEAVARAY LEGLGGEQIS KAVSPIAARW QGAWKQAKLA GDKAARAEIE AFRADVISYR
     NAWQFLSQIV DYDDPALAER AVLTTLLARR LHTDGIEIDM SYLDGVQLTG VKLVPSAIGE
     DHSLREGSGE AIPLPAFDGE RGGGVGSAPK RGPLDEAIDA VNELFSAKGV DVSSDSVAGF
     ITAFWGFLDA DDDAVAMARN NSAAQMKASE SFNNAVGLAM LKAVRESQEI QSYMTDPSFM
     DDIAEIAANA LHAQHQEDAD GRVEAGA
//