ID A0A1S1N0S5_9GAMM Unreviewed; 1160 AA.
AC A0A1S1N0S5;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=BET10_12640 {ECO:0000313|EMBL:OHU91646.1};
OS Pseudoalteromonas amylolytica.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC Pseudoalteromonadaceae; Pseudoalteromonas.
OX NCBI_TaxID=1859457 {ECO:0000313|EMBL:OHU91646.1, ECO:0000313|Proteomes:UP000179786};
RN [1] {ECO:0000313|EMBL:OHU91646.1, ECO:0000313|Proteomes:UP000179786}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JW1 {ECO:0000313|EMBL:OHU91646.1,
RC ECO:0000313|Proteomes:UP000179786};
RA Wu Y.-H., Cheng H., Jin X.-B., Wang C.-S., Xu X.-W.;
RT "Pseudoalteromonas amylolytica sp. nov., isolated from the surface
RT seawater.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OHU91646.1}.
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DR EMBL; MKJU01000025; OHU91646.1; -; Genomic_DNA.
DR RefSeq; WP_070985560.1; NZ_MKJU01000025.1.
DR AlphaFoldDB; A0A1S1N0S5; -.
DR STRING; 1859457.BET10_12640; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000179786; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 627..788
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 809..963
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1160 AA; 130758 MW; 00DDFA8432FC6BCF CRC64;
MAFAHWSELP WVKNTTDKIH WGQLSGSGLS VALSEGISKH PELVVIVTQD TPCALRLETE
LGYLLPKRSV MIFPDWETLP YDHFSPHQDI ISQRLATLNA LKQQNNSVLI VPVSTLMLRT
APPAFIYGSA LKYKVGDVID AQQLKSALSE SGYRHVQQVM EHGEFAVRGS IVDLYPMGSE
HPYRFDFFDD ELDSIRLFDI ETQRSSDKVD EIDLLPAHEF PTNDADIERF RVGYREAFGA
SAEKDSIYMQ VTKGNWPAGI EYYLPLFYEQ LATIFDYLPD NTTIMHLGDI EHAAQSFWQD
VEKRYENRRV DPLRPLLEPV KLYQPIEELY SQFNRYGRIR LSQAKLGTKA GHTNLSANEL
LDIRIDHKQS DPYSKILSYV AEQKKQKARI IFSVESDGRR ESLLTLLKPT ALKLKEFESF
ADFMASNTDV GLIVSPLEQS VFLSGKDSKP ALSIITEQEL LGVKVSQRRR RKHKYEQGQD
ALIRNLAELK EGQPIVHLDH GVGRYLGLQT IDAAGVTTEF VTITYANDAK LYVPVSALHM
LSRYSGGEEA TAPLHKLGSD VWEKAKRRAA EKVRDVAAEL LDIYAKRQSK PGYAFKLDGA
LYRDFANSFP FEETDDQRNA IEAVLADMQS NQAMDRLVCG DVGFGKTEVA MRGAFAAIND
NKQVAVLVPT TLLAQQHYEN FKDRFADLPV QVGVLSRFNS AKEQKDTLDK LESGQIDIVI
GTHKLIQENI KFKDLGLLIV DEEHRFGVRQ KEKIKQLRAD VDILTLTATP IPRTLNMAMS
GMRDLSIIAT PPAKRLAVKT FVKERNDELI REAILREIKR GGQVYFLHNN VETIDKTASD
IAALVPEAGV AIAHGQMRER ELEHLMSEFY HQKYNVLVCT TIIETGIDIP TANTIIMDRA
DKLGLAQMHQ LRGRVGRSHH QAYAYLLTRN SQTLTKDAVK RLQAIESLED LGAGFALATH
DLEIRGAGEL LGDDQSGQIQ TVGFTLYMEM LEQAVQALKD GKEPTLDNLL QQHTDVDLKI
PALLPDDYIP DVNMRLSMYK RIASTKSNQE LDELQVELID RFGLLPDAAK NLFKVQELKV
QASEIGVSKI EANAKGGYFE FNATTKVNPT FIIALIQSNP RVYKMEGANK LRFAIEEKNG
QERLKLINAM IEDFHKKAVA
//
