ID   A0A1S1Q342_9ACTN        Unreviewed;       340 AA.
AC   A0A1S1Q342;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   27-MAR-2024, entry version 29.
DE   SubName: Full=Radical SAM protein {ECO:0000313|EMBL:OHV27532.1};
GN   ORFNames=BCD49_38750 {ECO:0000313|EMBL:OHV27532.1};
OS   Pseudofrankia sp. EUN1h.
OC   Bacteria; Actinomycetota; Actinomycetes; Frankiales; Frankiaceae;
OC   Pseudofrankia.
OX   NCBI_TaxID=1834515 {ECO:0000313|EMBL:OHV27532.1, ECO:0000313|Proteomes:UP000179465};
RN   [1] {ECO:0000313|Proteomes:UP000179465}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EUN1h {ECO:0000313|Proteomes:UP000179465};
RA   Ghodhbane-Gtari F., Swanson E., Gueddou A., Morris K., Hezbri K., Ktari A.,
RA   Nouioui I., Abebe-Akele F., Thomas K., Gtari M., Tisa L.S.;
RT   "Sequence Frankia sp. strain EUN1h.";
RL   Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OHV27532.1}.
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DR   EMBL; MBLN01000128; OHV27532.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1S1Q342; -.
DR   Proteomes; UP000179465; Unassembled WGS sequence.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01335; Radical_SAM; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR007197; rSAM.
DR   PANTHER; PTHR11228:SF7; PQQA PEPTIDE CYCLASE; 1.
DR   PANTHER; PTHR11228; RADICAL SAM DOMAIN PROTEIN; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   4: Predicted;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT   DOMAIN          1..244
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
SQ   SEQUENCE   340 AA;  36708 MW;  30269499A81DD7CE CRC64;
     MTLLWALRSP CPLSCRYCYF GTIEEHRQTP VTGPGTLSHL SRTDLTAAEV LAFARTVAGS
     AVRRVFLAGG EPLVWPPVFQ VIETLKRADV QVVVCTNGLP LNRPDIVSTL LDLDVDAVSV
     SVDSADPAHH DAWRPSRNGK DGWRQVVTGI RSLVAARAAG ASPRVGVYMV VTRQNVTAMA
     ETARLAADLS CDYFVPQPIA LDQSHQLHHQ LSLTVADLPD LRGQFAALAE AGLPLRMPTA
     AYPEQVASSV REPVGVAYGC FGGSSLFFVE PDGSVWDCPS SLRIAATPTI RHRTIRGANA
     TEVFAGTDDC NDCPLFSVDC VNMWPLLDFD RFLTPIGSQP
//