ID A0A1S1XY02_9GAMM Unreviewed; 706 AA.
AC A0A1S1XY02;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=GTP pyrophosphokinase {ECO:0000256|ARBA:ARBA00019852};
DE EC=2.7.6.5 {ECO:0000256|ARBA:ARBA00013251};
DE AltName: Full=(p)ppGpp synthase {ECO:0000256|ARBA:ARBA00032407};
DE AltName: Full=ATP:GTP 3'-pyrophosphotransferase {ECO:0000256|ARBA:ARBA00029754};
DE AltName: Full=ppGpp synthase I {ECO:0000256|ARBA:ARBA00033308};
GN Name=relA {ECO:0000313|EMBL:OHX34126.1};
GN ORFNames=BJL95_06465 {ECO:0000313|EMBL:OHX34126.1};
OS Methylomonas sp. LWB.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC Methylococcaceae; Methylomonas.
OX NCBI_TaxID=1905845 {ECO:0000313|EMBL:OHX34126.1, ECO:0000313|Proteomes:UP000180009};
RN [1] {ECO:0000313|EMBL:OHX34126.1, ECO:0000313|Proteomes:UP000180009}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LWB {ECO:0000313|EMBL:OHX34126.1,
RC ECO:0000313|Proteomes:UP000180009};
RA Stephenson J., Kumaresan D., Hillebrand-Voiculescu A.M., Murrell J.C.;
RT "Draft genome sequence of the methane oxidising bacterium Candidatus
RT Methylomonas sp. LWB isolated from Movile Cave.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In eubacteria ppGpp (guanosine 3'-diphosphate 5'-diphosphate)
CC is a mediator of the stringent response that coordinates a variety of
CC cellular activities in response to changes in nutritional abundance.
CC {ECO:0000256|RuleBase:RU003847}.
CC -!- PATHWAY: Purine metabolism; ppGpp biosynthesis; ppGpp from GTP: step
CC 1/2. {ECO:0000256|ARBA:ARBA00004976}.
CC -!- SIMILARITY: Belongs to the relA/spoT family.
CC {ECO:0000256|RuleBase:RU003847}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OHX34126.1}.
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DR EMBL; MKMC01000100; OHX34126.1; -; Genomic_DNA.
DR RefSeq; WP_071160957.1; NZ_MKMC01000100.1.
DR AlphaFoldDB; A0A1S1XY02; -.
DR Proteomes; UP000180009; Unassembled WGS sequence.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0015969; P:guanosine tetraphosphate metabolic process; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd04876; ACT_RelA-SpoT; 1.
DR CDD; cd05399; NT_Rel-Spo_like; 1.
DR CDD; cd01668; TGS_RSH; 1.
DR Gene3D; 3.10.20.30; -; 1.
DR Gene3D; 3.30.70.260; -; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR Gene3D; 1.10.3210.10; Hypothetical protein af1432; 1.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR012675; Beta-grasp_dom_sf.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR004811; RelA/Spo_fam.
DR InterPro; IPR007685; RelA_SpoT.
DR InterPro; IPR004095; TGS.
DR InterPro; IPR012676; TGS-like.
DR InterPro; IPR033655; TGS_RelA/SpoT.
DR NCBIfam; TIGR00691; spoT_relA; 1.
DR PANTHER; PTHR21262:SF40; GTP PYROPHOSPHOKINASE; 1.
DR PANTHER; PTHR21262; GUANOSINE-3',5'-BIS DIPHOSPHATE 3'-PYROPHOSPHOHYDROLASE; 1.
DR Pfam; PF13291; ACT_4; 1.
DR Pfam; PF13328; HD_4; 1.
DR Pfam; PF04607; RelA_SpoT; 1.
DR Pfam; PF02824; TGS; 1.
DR SMART; SM00954; RelA_SpoT; 1.
DR SUPFAM; SSF55021; ACT-like; 1.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF81271; TGS-like; 1.
DR PROSITE; PS51671; ACT; 1.
DR PROSITE; PS51880; TGS; 1.
PE 3: Inferred from homology;
KW Kinase {ECO:0000313|EMBL:OHX34126.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000180009};
KW Transferase {ECO:0000313|EMBL:OHX34126.1}.
FT DOMAIN 382..445
FT /note="TGS"
FT /evidence="ECO:0000259|PROSITE:PS51880"
FT DOMAIN 633..706
FT /note="ACT"
FT /evidence="ECO:0000259|PROSITE:PS51671"
SQ SEQUENCE 706 AA; 79612 MW; F6FED9FA390BC796 CRC64;
MSEHTELALS LFPDFTGDDR LRIADALNLV DELGMATPAD ALPPRPKGID VAAILTSVHI
DLETILAAVL SDSRLTGLLS DTELRRRFGD VVADLVKDVH WLNNVSIYSP DMANEPNQTE
TLRRMLLAMT HDVRAVLIKL AYRIQRLRNL FREQTEIRHF ISRESLDIYA PLANRLGISQ
FKWELEDLAF RYLEPEQYRA ITESLASKRE EREACIEAFL AMLRQTLDRE GIAAKIYGRP
KHIYSIWHKM RRKQLAVDDL YDLLAVRVIV DTLPSCYTVL GVAHSHWRHI PKEFDDYIAN
PKENGYQSLH TVVLDEAGNR IEIQIRTQAM HEFAELGVAA HWRYKEGSKF NAATEKNIAS
LRQLLEDKDN GNLLENFHTE LFSDRVFVLT PAGKLIDLIR SATPLDFAYA IHTEVGHGCR
GAKINGQIKP LTYKLQSGEQ VEIITVKNGN PNHNWLNPNL GYLKTPRAIS KVKSWFKHQQ
QVANIASGKQ VLEKESKRLG IKAIDIDELI KHFKTPDAEH LYEALGRGDI NNRQLAGALK
IPELEPIPIK LASTQSATQA AVIVEDIDNV VTTLAQCCSP VKGDEIVGYI SHKRGITVHR
SDCENIRHLT PEQQLHVIKA DWSGHHKIHH NVPILIHAYN APNLLGDVSQ ILTASKAHIS
NASLKTHDDL SAILQMSIQI ENTAQLSSIL ARISHLPNIV EVKRKV
//
