ID   A0A1S1Y929_9GAMM        Unreviewed;       723 AA.
AC   A0A1S1Y929;
DT   12-APR-2017, integrated into UniProtKB/TrEMBL.
DT   12-APR-2017, sequence version 1.
DT   27-MAR-2024, entry version 19.
DE   SubName: Full=Glycogen debranching enzyme GlgX {ECO:0000313|EMBL:OHX38037.1};
GN   ORFNames=BJL95_07075 {ECO:0000313|EMBL:OHX38037.1};
OS   Methylomonas sp. LWB.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Methylococcales;
OC   Methylococcaceae; Methylomonas.
OX   NCBI_TaxID=1905845 {ECO:0000313|EMBL:OHX38037.1, ECO:0000313|Proteomes:UP000180009};
RN   [1] {ECO:0000313|EMBL:OHX38037.1, ECO:0000313|Proteomes:UP000180009}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LWB {ECO:0000313|EMBL:OHX38037.1,
RC   ECO:0000313|Proteomes:UP000180009};
RA   Stephenson J., Kumaresan D., Hillebrand-Voiculescu A.M., Murrell J.C.;
RT   "Draft genome sequence of the methane oxidising bacterium Candidatus
RT   Methylomonas sp. LWB isolated from Movile Cave.";
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000256|ARBA:ARBA00004229}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OHX38037.1}.
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DR   EMBL; MKMC01000005; OHX38037.1; -; Genomic_DNA.
DR   RefSeq; WP_071155270.1; NZ_MKMC01000005.1.
DR   AlphaFoldDB; A0A1S1Y929; -.
DR   Proteomes; UP000180009; Unassembled WGS sequence.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0019156; F:isoamylase activity; IEA:UniProt.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   InterPro; IPR048650; ISOA1-3-like_C.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 2.
DR   Pfam; PF02922; CBM_48; 1.
DR   Pfam; PF21156; ISOA1-3_C; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW   Plastid {ECO:0000256|ARBA:ARBA00022640};
KW   Reference proteome {ECO:0000313|Proteomes:UP000180009};
KW   Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT   DOMAIN          159..570
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
SQ   SEQUENCE   723 AA;  81297 MW;  58FFAA497C980676 CRC64;
     MHKQYHLKSG SPYPHGAKVG EGGVNFSISS RHATYVELLL FAQSDSEKPF QIIPLTTDKH
     HTFFSWHVFV VELPVGTWYA WRLDGPSQTR ETGLRFDRDK LLLDPWARAV SDKLWVRGAA
     CVPGDNSYHA MRGVVVDDHY DWEGDTPLSI RSEKVIVYEL HVGGFTRHPS AKVKHPGTFL
     GLIEKIPYLK KLGITHVELL PAMAFDEQDV PPHTAERGLT NYWGYSTHSF FSPHPGYCVT
     PEQGTHIREF RDLVRALHKA GIGVIMDVVF NHTSEAGSDG PVINFKGITG NSFYLTDKYD
     KRLFLDYTGC GNTVNANHPL VTNFIITCLE YWVREMHVDG FRFDLASALA RGEDGSVLQD
     PPLVWGIELS QQLAKTKLIA EAWDAAGLYQ VGNFPGYRWA EWNGRYRDAI RRFVRGDGGM
     INEVATRICG SSDLYEHQHR LPISGINFVT CHDGFTLHDL FSYNDKHNEA NGEENRDGCN
     NNLSYNCGVE GPTDDPEILA VRRRRAKNAL AILLLSHGVP MLLAGDEFLN SQGGNNNGYC
     QDNELSWLNW EDAKKNADVL RFVQTLIKLR KRHTALMRRR FLTGRPLEGK TLPDIHWYDV
     DQMHPPAWTD PEARFLSYTL SALAKEEPDL HVVMNMASED VLIDLPKIEG KAWCLSIDTA
     LSSPADAVEL ANQSPLSLQQ YRVRGNSVVV LENTRFSNEG GGESVSHKAS AFLTKLTGIR
     LID
//