ID A0A1S2D8I0_9GAMM Unreviewed; 717 AA.
AC A0A1S2D8I0;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 22.
DE RecName: Full=ATP-dependent DNA helicase DinG {ECO:0000256|HAMAP-Rule:MF_02205};
DE EC=3.6.4.12 {ECO:0000256|HAMAP-Rule:MF_02205};
GN Name=dinG {ECO:0000256|HAMAP-Rule:MF_02205};
GN ORFNames=BC443_17420 {ECO:0000313|EMBL:OHY97506.1};
OS Salinicola sp. MIT1003.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Salinicola.
OX NCBI_TaxID=1882734 {ECO:0000313|EMBL:OHY97506.1, ECO:0000313|Proteomes:UP000179615};
RN [1] {ECO:0000313|EMBL:OHY97506.1, ECO:0000313|Proteomes:UP000179615}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MIT1003 {ECO:0000313|EMBL:OHY97506.1,
RC ECO:0000313|Proteomes:UP000179615};
RA Biller S.;
RT "Membrane vesicles in seawater: heterogeneous DNA content and implications
RT for viral abundance estimates.";
RL Submitted (JUL-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase.
CC {ECO:0000256|HAMAP-Rule:MF_02205}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_02205};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_02205};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000256|HAMAP-Rule:MF_02205};
CC -!- SIMILARITY: Belongs to the helicase family. DinG subfamily. Type 1 sub-
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_02205}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OHY97506.1}.
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DR EMBL; MCAP01000059; OHY97506.1; -; Genomic_DNA.
DR RefSeq; WP_071233029.1; NZ_MCAP01000059.1.
DR AlphaFoldDB; A0A1S2D8I0; -.
DR STRING; 1882734.BC443_17420; -.
DR OrthoDB; 9805194at2; -.
DR Proteomes; UP000179615; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006139; P:nucleobase-containing compound metabolic process; IEA:InterPro.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_02205; DinG_proteobact; 1.
DR InterPro; IPR006555; ATP-dep_Helicase_C.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR045028; DinG/Rad3-like.
DR InterPro; IPR039000; DinG_proteobact.
DR InterPro; IPR014013; Helic_SF1/SF2_ATP-bd_DinG/Rad3.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR010614; RAD3-like_helicase_DEAD.
DR PANTHER; PTHR11472:SF59; ATP-DEPENDENT DNA HELICASE DING; 1.
DR PANTHER; PTHR11472; DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF06733; DEAD_2; 1.
DR Pfam; PF13307; Helicase_C_2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00491; HELICc2; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51193; HELICASE_ATP_BIND_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|HAMAP-Rule:MF_02205};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_02205}; DNA-binding {ECO:0000256|HAMAP-Rule:MF_02205};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|HAMAP-Rule:MF_02205};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_02205};
KW Iron {ECO:0000256|HAMAP-Rule:MF_02205};
KW Iron-sulfur {ECO:0000256|HAMAP-Rule:MF_02205};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_02205};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_02205}; Reference proteome {ECO:0000313|Proteomes:UP000179615}.
FT DOMAIN 15..318
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51193"
FT BINDING 129
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02205"
FT BINDING 203
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02205"
FT BINDING 208
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02205"
FT BINDING 214
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02205"
SQ SEQUENCE 717 AA; 79323 MW; EFFFCE1A03EFC16E CRC64;
MLDEPLKQEI QEAYRHVLEG LELTPRYGQR LMIAEIARTL ASIEQDEQGR RTSDEHVCVL
EAGTGTGKTL AYLLAALPVA KARGKRLVVA TATVALQEQV LNQDLPMLKA HSGLDFEYAL
AKGRGRYLCV AKLDQALEGA EPNPTLSLFE QTIATDAGGD FPQLIQEMAD TYASGNWEGD
RDSWPVAIDD RDWRRLTVDH RQCTNRRCGH FGACAYFRSR RHLEQADIIV ANHDLVLADL
ALGGGMVLPS PKDCIYVFDE GHHLPDKALD HFYHRFGING TLRWLRTLKK SLTELNTALG
AQPTLGRLLA TFPERIAALE PRLGDAYAMG HQIAGLGSGA GEETQHFRFP NGRAPEPLVD
LAQQLVIPFA ELSRDLETMS DILRESLDPD KSTGLPREQA EPWLPLTALL HGRALEAHAL
WSAMAKPDPA GQAPQARWLT FQNFGGEPEL TFSASPVSAA ETLARHLWGT CYGAVVTSAT
LTALNRFERL QERAGLANRY RYQRLPSPFD YSRAILSVPR EAVEPGDAKA HDRSIVEFIE
GLEDDEAVLV LFSSRKQLRS VADALPEPWA SRLLSQDRLP KRELLERHRA AVDADSGSLI
FGLASFAEGI DLPGRYLTHV VITRLPFSVP DDPVGATLAE WIESQGGNPF MRISVPDASI
KLVQACGRLI RKENDSGRIS LLDRRVLTRR YGKALLDALP PFRRQIEGVE AGEPARN
//
