ID A0A1S2KXN3_9BACI Unreviewed; 795 AA.
AC A0A1S2KXN3;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=3-hydroxyacyl-CoA dehydrogenase {ECO:0000313|EMBL:OIJ04337.1};
DE SubName: Full=Enoyl-CoA hydratase/isomerase family protein {ECO:0000313|EMBL:QOY36846.1};
GN ORFNames=AWH56_004110 {ECO:0000313|EMBL:QOY36846.1}, AWH56_23255
GN {ECO:0000313|EMBL:OIJ04337.1};
OS Anaerobacillus isosaccharinicus.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Anaerobacillus.
OX NCBI_TaxID=1532552 {ECO:0000313|EMBL:OIJ04337.1, ECO:0000313|Proteomes:UP000180175};
RN [1] {ECO:0000313|EMBL:OIJ04337.1, ECO:0000313|Proteomes:UP000180175}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NB2006 {ECO:0000313|EMBL:OIJ04337.1,
RC ECO:0000313|Proteomes:UP000180175};
RA Bassil N.M., Lloyd J.R.;
RT "Draft genome sequences of four alkaliphilic bacteria belonging to the
RT Anaerobacillus genus.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:QOY36846.1, ECO:0000313|Proteomes:UP000180175}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NB2006 {ECO:0000313|EMBL:QOY36846.1,
RC ECO:0000313|Proteomes:UP000180175};
RX PubMed=28104661;
RA Bassil N.M., Lloyd J.R.;
RT "Draft Genome Sequences of Four Alkaliphilic Bacteria Belonging to the
RT Anaerobacillus Genus.";
RL Genome Announc. 5:e01493-16(2017).
RN [3] {ECO:0000313|EMBL:QOY36846.1, ECO:0000313|Proteomes:UP000180175}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NB2006 {ECO:0000313|EMBL:QOY36846.1,
RC ECO:0000313|Proteomes:UP000180175};
RX PubMed=29580368;
RA Bassil N.M., Lloyd J.R.;
RT "Anaerobacillus isosaccharinicus sp. nov., an alkaliphilic bacterium which
RT degrades isosaccharinic acid.";
RL Int. J. Syst. Evol. Microbiol. 69:3666-3671(2019).
RN [4] {ECO:0000313|EMBL:QOY36846.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NB2006 {ECO:0000313|EMBL:QOY36846.1};
RA Bassil N.M., Lloyd J.R.;
RL Submitted (OCT-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
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DR EMBL; LQXD01000201; OIJ04337.1; -; Genomic_DNA.
DR EMBL; CP063356; QOY36846.1; -; Genomic_DNA.
DR RefSeq; WP_071319305.1; NZ_CP063356.1.
DR AlphaFoldDB; A0A1S2KXN3; -.
DR KEGG; aia:AWH56_004110; -.
DR OrthoDB; 9771883at2; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000180175; Chromosome.
DR Proteomes; UP000180175; Unassembled WGS sequence.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR48075; 3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN; 1.
DR PANTHER; PTHR48075:SF7; 3-HYDROXYACYL-COA DEHYDROGENASE-RELATED; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 4: Predicted;
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Isomerase {ECO:0000313|EMBL:QOY36846.1};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Reference proteome {ECO:0000313|Proteomes:UP000180175}.
FT DOMAIN 7..205
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 208..305
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 795 AA; 87612 MW; 02A05191D0C92867 CRC64;
MIGRIGKVAI LGSGVMGSGI AAHLANIGIP TLLLDIVPRD LTEEEKQRGL TLQDMQVRNR
LAQTSIQKLL KQKPAPLTEK GHIDLIEAGN MEDDMKRLSE VDWIIEVVVE NLEIKKKVFA
QVDEYRKQGS IVSSNTSGIS IEAMAEGRSE DFRKHFLGTH FFNPPRYLKL LEVIRTKETS
EEVFTFMKQF GEDVLGKGVV EAKDTPNFIA NRIGTYGLLV TVAEMVKGGY SVGEVDSVTG
PALGRPKSAT FRTLDVVGLD TFLHVAKNVY DQVDGVEKTV FDPPQFMRDM AEKGWLGSKS
GQGFFLKKKG ANGSEILELD YNTMEYVQSK KLKAASVQAS KQAKGKSEKL KALVYAKDRA
GELVWNILKP VLLYSAEKAT EIADDILSID QAMKWGFGWE LGPFETWDAI GVAKSVEKME
AEGEQVPLWV KEMLEKGHKS FYEGRNCFYN AGEYAQAAEN PKVIHIKTLK ENNNVIKKNS
GASLIDIGDG VACLEFTSPN NSIGLDVMQM INYSIDEVAK NYKGLVINNQ GKNFCVGANL
MMILMEAQDD NFFEIDLVVR QFQQSMAKIR YSARPVVTAP FGMTLGGGTE ICLPSARIQA
SSETYMGLVE VGVGLIPGGG GNKELYLRLL EGLPEGAQID LQAIANRTFE TIAMAKVSTS
AHEAAKLGFI RPQDEISMNG DHLLHDAKDH VLHLSNQGYQ PPQRKKIPVV GESGYATMLL
GAKTMQFGGM ISDHDLKIAQ KLAFTIAGGR VPKGTLVDEQ YLLDIEREAF LSLVGEPKTQ
MRMQHMLTKG KPLRN
//
