ID A0A1S2MDA1_9BACI Unreviewed; 295 AA.
AC A0A1S2MDA1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE SubName: Full=5'-3' exonuclease {ECO:0000313|EMBL:QOY38745.1};
DE SubName: Full=Flap endonuclease {ECO:0000313|EMBL:OIJ22556.1};
GN ORFNames=AWH56_023785 {ECO:0000313|EMBL:QOY38745.1}, AWH56_05730
GN {ECO:0000313|EMBL:OIJ22556.1};
OS Anaerobacillus isosaccharinicus.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Anaerobacillus.
OX NCBI_TaxID=1532552 {ECO:0000313|EMBL:OIJ22556.1, ECO:0000313|Proteomes:UP000180175};
RN [1] {ECO:0000313|EMBL:OIJ22556.1, ECO:0000313|Proteomes:UP000180175}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NB2006 {ECO:0000313|EMBL:OIJ22556.1,
RC ECO:0000313|Proteomes:UP000180175};
RA Bassil N.M., Lloyd J.R.;
RT "Draft genome sequences of four alkaliphilic bacteria belonging to the
RT Anaerobacillus genus.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:QOY38745.1, ECO:0000313|Proteomes:UP000180175}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NB2006 {ECO:0000313|EMBL:QOY38745.1,
RC ECO:0000313|Proteomes:UP000180175};
RX PubMed=28104661;
RA Bassil N.M., Lloyd J.R.;
RT "Draft Genome Sequences of Four Alkaliphilic Bacteria Belonging to the
RT Anaerobacillus Genus.";
RL Genome Announc. 5:e01493-16(2017).
RN [3] {ECO:0000313|EMBL:QOY38745.1, ECO:0000313|Proteomes:UP000180175}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NB2006 {ECO:0000313|EMBL:QOY38745.1,
RC ECO:0000313|Proteomes:UP000180175};
RX PubMed=29580368;
RA Bassil N.M., Lloyd J.R.;
RT "Anaerobacillus isosaccharinicus sp. nov., an alkaliphilic bacterium which
RT degrades isosaccharinic acid.";
RL Int. J. Syst. Evol. Microbiol. 69:3666-3671(2019).
RN [4] {ECO:0000313|EMBL:QOY38745.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=NB2006 {ECO:0000313|EMBL:QOY38745.1};
RA Bassil N.M., Lloyd J.R.;
RL Submitted (OCT-2020) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; LQXD01000054; OIJ22556.1; -; Genomic_DNA.
DR EMBL; CP063356; QOY38745.1; -; Genomic_DNA.
DR RefSeq; WP_071316224.1; NZ_CP063356.1.
DR AlphaFoldDB; A0A1S2MDA1; -.
DR KEGG; aia:AWH56_023785; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000180175; Chromosome.
DR Proteomes; UP000180175; Unassembled WGS sequence.
DR GO; GO:0017108; F:5'-flap endonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0033567; P:DNA replication, Okazaki fragment processing; IEA:InterPro.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR038969; FEN.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR PANTHER; PTHR42646:SF2; DNA-DIRECTED DNA POLYMERASE; 1.
DR PANTHER; PTHR42646; FLAP ENDONUCLEASE XNI; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
PE 4: Predicted;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000313|EMBL:OIJ22556.1};
KW Exonuclease {ECO:0000313|EMBL:QOY38745.1};
KW Hydrolase {ECO:0000313|EMBL:QOY38745.1};
KW Nuclease {ECO:0000313|EMBL:QOY38745.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000180175}.
FT DOMAIN 1..259
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
SQ SEQUENCE 295 AA; 33298 MW; 447324821AC64397 CRC64;
MIIDGMALLF RGYYATSYSG YIMKTSKGVP TNAIYGFVKY MQDAISTFQP SHVLCCWDMG
ARTFRNELYP AYKANRGEPP EELIPQFDLV KKVVESFNIP SVGIEGFEAD DCIGTIAKKY
SQHLKIQILT GDHDSLQLID ENIHSIIMKK GMSNYEVYTL EKLLEEKELT PAQFIDLKGL
MGDASDNFPG VKGIGEKTAI KLLKEYQSIS GILENLPSLS KGIRTKIETE LDMLHLSRKL
AEIHCEVPVE IVLEECARNI DEVKVTAMFE ELEFHRLLKD KSFLGNIKVN VQPLC
//
