ID A0A1S2XGT3_CICAR Unreviewed; 513 AA.
AC A0A1S2XGT3;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=O-acyltransferase WSD1-like {ECO:0000313|RefSeq:XP_004489155.1};
GN Name=LOC101488691 {ECO:0000313|RefSeq:XP_004489155.1};
OS Cicer arietinum (Chickpea) (Garbanzo).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Cicereae; Cicer.
OX NCBI_TaxID=3827 {ECO:0000313|Proteomes:UP000087171, ECO:0000313|RefSeq:XP_004489155.1};
RN [1] {ECO:0000313|RefSeq:XP_004489155.1}
RP IDENTIFICATION.
RC TISSUE=Etiolated seedlings {ECO:0000313|RefSeq:XP_004489155.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC Evidence={ECO:0000256|ARBA:ARBA00029340};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004162};
CC Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004162}. Membrane
CC {ECO:0000256|ARBA:ARBA00004167}; Single-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004167}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the long-chain O-
CC acyltransferase family. {ECO:0000256|ARBA:ARBA00024360}.
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DR RefSeq; XP_004489155.1; XM_004489098.2.
DR AlphaFoldDB; A0A1S2XGT3; -.
DR STRING; 3827.A0A1S2XGT3; -.
DR PaxDb; 3827-XP_004489155-1; -.
DR GeneID; 101488691; -.
DR KEGG; cam:101488691; -.
DR eggNOG; ENOG502QSH5; Eukaryota.
DR OrthoDB; 2957273at2759; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000087171; Chromosome Ca2.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR PANTHER; PTHR31650:SF29; O-ACYLTRANSFERASE WSD1-LIKE PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Reference proteome {ECO:0000313|Proteomes:UP000087171};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 111..302
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 356..501
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 513 AA; 58026 MW; F0AD08CBA0E26D90 CRC64;
MIICLFVHTI TYPNFLSFDL PIYSSISFSL NYHTLIIFYF CIMELEREEQ LEEPVSPIGQ
YFNSSVLCLY IIGVLEFEVP IDDLQTFSLL QDVFLPISPR FSSIMIQDKN GEKRWKQVDV
NLKDHVKTPI FSKGKTLEFY EKCFVDYLST IAMEQLPQER PLWEVHIVNY PTSDSLGTII
FKLHHALGDG YSLMGALLSC LQRADDSSLP LSFPSLRPSK SESSSKSIWI RFSRTMSSVF
NTASDFGWSV LKSSIIDDDK TPIRFGDEGA DFQPISISSI AFSIDHIKDI KSRLGVTLND
VVTGIVFYGT RLYMQNLDSK SKTANSTALV LLNTRNIEGY QSIDEMLNTK KTKGPWGNKI
SFLHVPIPKL NGSNISNPLE FIWDTHNIIK RKKQSLGVAL TGTLLDIEGK FRGQEAVAKH
IRRTITNSSA VVTNLVGPIQ KMSLANHPVK GFYFTLAGGP ESLVISIMSY MEVVRVTLKT
EKEFIDEQRL KSCMQSSFEM IFKAAKKIPH HTN
//
