ID A0A1S2YNL3_CICAR Unreviewed; 580 AA.
AC A0A1S2YNL3;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=(R)-mandelonitrile lyase-like {ECO:0000313|RefSeq:XP_004507539.1};
GN Name=LOC101508164 {ECO:0000313|RefSeq:XP_004507539.1};
OS Cicer arietinum (Chickpea) (Garbanzo).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Cicereae; Cicer.
OX NCBI_TaxID=3827 {ECO:0000313|Proteomes:UP000087171, ECO:0000313|RefSeq:XP_004507539.1};
RN [1] {ECO:0000313|RefSeq:XP_004507539.1}
RP IDENTIFICATION.
RC TISSUE=Etiolated seedlings {ECO:0000313|RefSeq:XP_004507539.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|PIRSR:PIRSR000137-2};
CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC {ECO:0000256|ARBA:ARBA00010790}.
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DR RefSeq; XP_004507539.1; XM_004507482.3.
DR AlphaFoldDB; A0A1S2YNL3; -.
DR STRING; 3827.A0A1S2YNL3; -.
DR PaxDb; 3827-XP_004507539-1; -.
DR GeneID; 101508164; -.
DR KEGG; cam:101508164; -.
DR eggNOG; KOG1238; Eukaryota.
DR OrthoDB; 52047at2759; -.
DR Proteomes; UP000087171; Chromosome Ca6.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0016614; F:oxidoreductase activity, acting on CH-OH group of donors; IEA:InterPro.
DR Gene3D; 3.30.410.40; -; 1.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR012132; GMC_OxRdtase.
DR InterPro; IPR000172; GMC_OxRdtase_N.
DR InterPro; IPR007867; GMC_OxRtase_C.
DR PANTHER; PTHR45968:SF2; (R)-MANDELONITRILE LYASE-LIKE; 1.
DR PANTHER; PTHR45968; OSJNBA0019K04.7 PROTEIN; 1.
DR Pfam; PF05199; GMC_oxred_C; 1.
DR Pfam; PF00732; GMC_oxred_N; 1.
DR PIRSF; PIRSF000137; Alcohol_oxidase; 2.
DR SUPFAM; SSF54373; FAD-linked reductases, C-terminal domain; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR PROSITE; PS00624; GMC_OXRED_2; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR000137-3};
KW FAD {ECO:0000256|PIRSR:PIRSR000137-2};
KW Flavoprotein {ECO:0000256|PIRSR:PIRSR000137-2};
KW Lyase {ECO:0000313|RefSeq:XP_004507539.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000087171};
KW Signal {ECO:0000256|ARBA:ARBA00022729}.
FT DOMAIN 313..327
FT /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS00624"
FT BINDING 92..93
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT BINDING 144
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT BINDING 259
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT BINDING 514..515
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT BINDING 543
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT BINDING 554..555
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-2"
FT DISULFID 455..506
FT /evidence="ECO:0000256|PIRSR:PIRSR000137-3"
SQ SEQUENCE 580 AA; 63745 MW; D522C5A9A9A7D9D3 CRC64;
MEKSAITVTT EFLFLTSFIT IFIGSAFASP TQNQESNSQE QQDKPPSYLR MVANASEFPL
EDYYDYIIVG GGTAGCPLAA TLSQNYRVLV LERGGVTHGK SNLMNQEGFL NTLLNANVNN
ANNEDSPAQS FISEDGVLNA RGRVLGGSSA INAGFYSRAD HEFFTRSGVI WDSKLVNESY
EWVEREIVFR PQLKTWQSAF RDGLLEARVG PYNGFTLDHA TGTKIGGSTF DTSGKRHSSA
DLLRYARHSN LRIAVYASVE RILLASSSSS TTTSSETSSS ATTSAIGVLY RDQNGKYHHA
ILRGRGEVIL AAGAIGSPQL LLLSGIGPRP YLSSWGIPVT HHLPYVGHFL YDNPRNGITF
LPSIPLEHSL IQVVGITNSG AYIEAASNVV PFSSPVRNFF IRESNSPLYL TVATLISKIS
GPVSAGFLRL ASTDVRFNPI VRFNYFNNGV DVERCVNGTR KLGEVLRSRA MNDFKFVNWL
GVRDFRFIGP ALPNDESDYI EMADFCRRTV STIWHYHGGC VVGRVVDSHL KVIGIDSLRI
VDGSVFSVSP GTNPQATLMM LGRYFGLKMI REREGYSNDL
//
