ID A0A1S3AA13_ERIEU Unreviewed; 602 AA.
AC A0A1S3AA13;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Hyaluronidase {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
DE EC=3.2.1.35 {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
GN Name=LOC103121175 {ECO:0000313|RefSeq:XP_007531753.1};
OS Erinaceus europaeus (Western European hedgehog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Eulipotyphla; Erinaceidae; Erinaceinae;
OC Erinaceus.
OX NCBI_TaxID=9365 {ECO:0000313|Proteomes:UP000079721, ECO:0000313|RefSeq:XP_007531753.1};
RN [1] {ECO:0000313|RefSeq:XP_007531753.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-
CC glucosamine and D-glucuronate residues in hyaluronate.; EC=3.2.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00000251,
CC ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004609};
CC Lipid-anchor, GPI-anchor {ECO:0000256|ARBA:ARBA00004609}. Membrane
CC {ECO:0000256|ARBA:ARBA00004589}; Lipid-anchor, GPI-anchor
CC {ECO:0000256|ARBA:ARBA00004589}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 56 family.
CC {ECO:0000256|ARBA:ARBA00008871, ECO:0000256|PIRNR:PIRNR038193,
CC ECO:0000256|RuleBase:RU610713}.
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DR RefSeq; XP_007531753.1; XM_007531691.1.
DR AlphaFoldDB; A0A1S3AA13; -.
DR STRING; 9365.ENSEEUP00000007795; -.
DR GlyCosmos; A0A1S3AA13; 1 site, No reported glycans.
DR eggNOG; ENOG502R6HD; Eukaryota.
DR InParanoid; A0A1S3AA13; -.
DR OrthoDB; 5344684at2759; -.
DR Proteomes; UP000079721; Unplaced.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0098552; C:side of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004415; F:hyalurononglucosaminidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IEA:InterPro.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR018155; Hyaluronidase.
DR InterPro; IPR001439; Hyaluronidase_PH20/Hyal5.
DR PANTHER; PTHR11769; HYALURONIDASE; 1.
DR PANTHER; PTHR11769:SF20; HYALURONIDASE PH-20; 1.
DR Pfam; PF01630; Glyco_hydro_56; 1.
DR PIRSF; PIRSF038193; Hyaluronidase; 1.
DR PIRSF; PIRSF500773; Hyaluronidase_PH20_Hyal5; 1.
DR PRINTS; PR00846; GLHYDRLASE56.
DR PRINTS; PR00848; SPERMPH20.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR038193-3};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00022622};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR038193};
KW GPI-anchor {ECO:0000256|ARBA:ARBA00022622};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
KW Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000079721};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 9..30
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 561..582
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT CARBOHYD 369
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-2"
FT DISULFID 60..352
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 224..238
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 377..388
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 382..436
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 438..444
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
SQ SEQUENCE 602 AA; 69147 MW; F691485ECCC5ECB8 CRC64;
MEILSSTPIF FNCFVGCSGI PGVVFIFLLF PVSLTRDFRA LPLIPDTPFL WAWNAPTEIC
EERFNEPIDL RFFSLVGSPQ KDITGQRITI FYFDRLGLYP YIDNVTLTNV NGGIPQKGSL
QNHLEKAKVD ILYYMPEDKI GLAVIDWREW NPLWERNWKP KDIYQQRSIE SVKQQNESLN
DSEANILAKE YFEEAAKNFM KETLKLGTSL RPNYIWGYYL FPDCYNNLYN TQDYDGSCSD
IEKNRNDELS WLWEESTALY PSIYLNSKLS STPEATLYAQ NRIQEAIRAS RAHNTEDPLP
IYIYTRPVFT DALGEYLPQT DLVSTIGEGV ALGASGTIMW GSFNVSQSKQ SCMDLETYMK
STLNPYLINV TLAAKMCSQV LCQEHGVCTR KSWNSSDYLH LNPEHFVIQS GEGGKYIVDG
KPTLEDLRYF YENFHCSCYP HINCHETLDI QNIDIINVCV ADDVCIEAFL GTEMNDLSSD
LEDEVSENPT DVESYVNSVC EPGVDYHECL KLKCSMQEMF ANGTDKECEG VNWQNVTSDL
HFLHKKENKT QIPKNPASIK FPIYILHFAY NLLFKLIANY IFSIFYESSA NIYTVSIYER
ND
//
