ID A0A1S3CDA0_CUCME Unreviewed; 496 AA.
AC A0A1S3CDA0;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN Name=LOC103499723 {ECO:0000313|RefSeq:XP_008461017.1};
GN Synonyms=103499723 {ECO:0000313|EnsemblPlants:MELO3C023353.2.1};
OS Cucumis melo (Muskmelon).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX NCBI_TaxID=3656 {ECO:0000313|Proteomes:UP000089565, ECO:0000313|RefSeq:XP_008461017.1};
RN [1] {ECO:0000313|Proteomes:UP000089565}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. DHL92 {ECO:0000313|Proteomes:UP000089565};
RX PubMed=22753475; DOI=10.1073/pnas.1205415109;
RA Garcia-Mas J., Benjak A., Sanseverino W., Bourgeois M., Mir G.,
RA Gonzalez V.M., Henaff E., Camara F., Cozzuto L., Lowy E., Alioto T.,
RA Capella-Gutierrez S., Blanca J., Canizares J., Ziarsolo P.,
RA Gonzalez-Ibeas D., Rodriguez-Moreno L., Droege M., Du L.,
RA Alvarez-Tejado M., Lorente-Galdos B., Mele M., Yang L., Weng Y.,
RA Navarro A., Marques-Bonet T., Aranda M.A., Nuez F., Pico B., Gabaldon T.,
RA Roma G., Guigo R., Casacuberta J.M., Arus P., Puigdomenech P.;
RT "The genome of melon (Cucumis melo L.).";
RL Proc. Natl. Acad. Sci. U.S.A. 109:11872-11877(2012).
RN [2] {ECO:0000313|EnsemblPlants:MELO3C023353.2.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (MAR-2023) to UniProtKB.
RN [3] {ECO:0000313|RefSeq:XP_008461017.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR RefSeq; XP_008461017.1; XM_008462795.2.
DR EnsemblPlants; MELO3C023353.2.1; MELO3C023353.2.1; MELO3C023353.2.
DR GeneID; 103499723; -.
DR Gramene; MELO3C023353.2.1; MELO3C023353.2.1; MELO3C023353.2.
DR KEGG; cmo:103499723; -.
DR eggNOG; ENOG502QV58; Eukaryota.
DR InParanoid; A0A1S3CDA0; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000089565; Unplaced.
DR Proteomes; UP000596662; Unplaced.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF129; ENDOGLUCANASE 24; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000089565};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 28..496
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5041474475"
FT DOMAIN 31..487
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 414
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 465
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 474
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 496 AA; 54590 MW; EF777C07025D7959 CRC64;
MKNPIFSHRT ILFLILSILL SPDFTFAGHD YTDALSKCIL FFEGQRSGFL PQDQRMTWRA
NSGLGDGWTY KTDLTGGYYD AGDNVKFGFP MAFTTTLLSW SVIEFGDLMP PAELRNSLVA
IRWATDYLLK TVSQPNRIFV QVGDPSADHF CWERPEDMDT MRTVYAVDAP GTASDVAGET
AAALAAASMA FRSSDPGYAE TLLQNGIKAF ELADTYRGAY SDNANIRDGV CPFYCDFDGY
QDELLWGAAW LRRASKNESY LSYIQDNGKT LGAEDSFNEF GWDNKHAGLN VLVSKEALEG
NIFTLQSYKA SADNFMCTLI PESSSSHIQY TPGGLIYKPG GSNLQHATSI TFLLLAYAHY
LERTSSTVNC GNVVVGPASL RRQAKQQVDY ILGDNPKGIS YMVGYGNYFP QRIHHRGSSL
PSVRDHPQPI ACKEGSTYFN SPDPNPNVLV GALVGGPGED DVYEDDRADF RKSEPTTYIN
APFVGVLAYF AANPGG
//
