ID A0A1S3NRQ1_SALSA Unreviewed; 2056 AA.
AC A0A1S3NRQ1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=E3 ubiquitin-protein ligase {ECO:0000256|RuleBase:RU369009};
DE EC=2.3.2.26 {ECO:0000256|RuleBase:RU369009};
GN Name=LOC106580884 {ECO:0000313|RefSeq:XP_014017886.1,
GN ECO:0000313|RefSeq:XP_014017887.1, ECO:0000313|RefSeq:XP_014017888.1};
OS Salmo salar (Atlantic salmon).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC Salmonidae; Salmoninae; Salmo.
OX NCBI_TaxID=8030 {ECO:0000313|Proteomes:UP000087266, ECO:0000313|RefSeq:XP_014017886.1};
RN [1] {ECO:0000313|RefSeq:XP_014017886.1, ECO:0000313|RefSeq:XP_014017887.1}
RP IDENTIFICATION.
RC TISSUE=Muscle {ECO:0000313|RefSeq:XP_014017886.1,
RC ECO:0000313|RefSeq:XP_014017887.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: E3 ubiquitin-protein ligase involved in ubiquitin fusion
CC degradation (UFD) pathway and regulation of DNA repair. Part of the
CC ubiquitin fusion degradation (UFD) pathway, a process that mediates
CC ubiquitination of protein at their N-terminus, regardless of the
CC presence of lysine residues in target proteins.
CC {ECO:0000256|RuleBase:RU369009}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885,
CC ECO:0000256|RuleBase:RU369009};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369009}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642, ECO:0000256|RuleBase:RU369009}.
CC -!- SIMILARITY: Belongs to the UPL family. K-HECT subfamily.
CC {ECO:0000256|ARBA:ARBA00006331, ECO:0000256|RuleBase:RU369009}.
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DR RefSeq; XP_014017886.1; XM_014162411.1.
DR RefSeq; XP_014017887.1; XM_014162412.1.
DR RefSeq; XP_014017888.1; XM_014162413.1.
DR STRING; 8030.ENSSSAP00000104143; -.
DR PaxDb; 8030-ENSSSAP00000104143; -.
DR GeneID; 106580884; -.
DR KEGG; sasa:106580884; -.
DR CTD; 9320; -.
DR OrthoDB; 1093891at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000087266; Chromosome ssa20.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.720.50; -; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR045322; HECTD1/TRIP12-like.
DR InterPro; IPR004170; WWE-dom.
DR InterPro; IPR018123; WWE-dom_subgr.
DR InterPro; IPR037197; WWE_dom_sf.
DR PANTHER; PTHR45670; E3 UBIQUITIN-PROTEIN LIGASE TRIP12; 1.
DR PANTHER; PTHR45670:SF1; E3 UBIQUITIN-PROTEIN LIGASE TRIP12; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF02825; WWE; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00678; WWE; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF117839; WWE domain; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50918; WWE; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU369009};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000087266};
KW Transferase {ECO:0000256|RuleBase:RU369009};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 794..870
FT /note="WWE"
FT /evidence="ECO:0000259|PROSITE:PS50918"
FT DOMAIN 1659..2056
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 1..442
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 865..886
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1038..1143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1470..1499
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1809..1843
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 1..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..83
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 106..131
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 135..152
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 192..254
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 311..327
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 353..374
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 380..395
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 396..437
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1038..1059
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1082..1096
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1097..1137
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 2023
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 2056 AA; 227039 MW; FFE650F433F34429 CRC64;
MSNRPNSNPG GSLRRSQRNT AAAQPQDHTV AGRLQSEQVK HKANSPPESR RPKSKASKAA
PSSASGQSRG HSSRSSLSLS VASFVLQDDP EAAGTSEREP TGHQSKTEGT TRGLKRNSAP
DLNTYTPTPA KKSKSNPPPR DHTTEAKKGP AKSSKKRPLA PELPTGCGQS KKSGASGASP
TLKRKKADSL PCLSSTAGSL PNRTDGRSAK PTKLASKSAA SAKAGCSTVT DSSSSASTSS
SSSTGTNSAT AAQGARLKQG KDQNKARRSR SASSPSPRRS TRDKEQAKTS VSSKFEWAAR
FSPKVNLPKP KLSLPSSSKT ETASKPGPSG LQAKLASLRK STKKRSESPP AELPSFRRST
RQKTTGSCAS TSRRGSGLGK RGAAEARRQE KMADSDNNQD GANSSAARTD EAPQGASGTA
SSSVTGAVGM TTSGESESDD SEMGRLQALL EARGLPPHLF GPLGPRMSQL FHRTIGSGAS
SKAQQLLQGL QATGDESQQL QAAIEMCQLL VMGNEETLGG FPVKSVVPAL ITLLQMEHNF
DIMNHASRAL TYMMEALPRS SAVVVDAIPV FLEKLQVIQF IDVAEQALTA LEMLSRRHSK
AILQAGGLAD CLLYLEFFSI NAQRNALAIA ANCCQSITPD EFHFVSDSLP LLTQRLTHQD
KKSVESTCLC FARLVDNFQH EENLLQQVAS RDLLTNIQQL LVVTPPVLSS GMFIMVVRMF
SLMCSNCPCL AVQLMKQNIA ETLRFLLCGA ANGSCQEQID LVPRSPQELY ELTSLICELM
PCLPREGIFA VDLMLKKGSA QATEGAIWQW RDDRGLWHPY NRIDSRIIET AHQNGEDEIS
LSTLGRVYTI DFNSMQQINE DTGTARGIQR KPNPLANPTT GGHQEVRRED ARAQLMKEDP
ELAKCFIKTL FGVLYEVYSS SAGPAVRHKC LRAILRIIFF ADAELLKDVL RNHAVSSHIA
SMLSSQDLKI VVGSLQMAEI LMQKLPDVFS VFFRREGVMH QVKNLAESEA FLTSPPKACT
SGTASLCTIS TANTTAATTA TPDLGSPSFQ HSMDDSLDLS PQGRLSDVLK RKRLPKRGPR
RPKYSPPRDD DKVDNQAKSP TTTQSPKSSF LASLNPKTWG KLGTQTNSTN SEPSRTAGVS
GLARALPKDS VSNNRDKIKA WIKEQACKFV ARYFNTENID GSNPALNVLQ RLCTATQQLS
LQMDGGVECL VEICSIVSES DVSSFEIQHS GLVKQLLLYL TSRSDRDLVS RDVRLKRFLH
VFYGCPIPGM EPAGRLEPSE NGPLLSLVRK MNNCLSQMEQ FPVKVHDFPS GNGNGSRGSQ
ALKFFNTHQL KCQLQRHPDC TNVKQWKGGP VKIDPLALVQ AIERYLVVRG YGRIREEDED
SDDDGSDDEI DESLAAQFLN SGSVRHRLQF YIGDHLLPYN MTVYQAVRQF SLQAEEERES
TDDEANPLGR AGIWTKTHTI WYKPVREDED GTKDVVGGKR GRAQTAPTKT SPRNAKKQDE
LWHDGVCPSV ASPLEMYLMS EPPESITFDD PSLDVNLLLR VLHSISSYWF YLYDNAVSKE
IIPTSEFINS KLTAKANRQL QDPLVIMTGN IPTWLTELGK TCPFFFPFDT RQMLFYVTAF
DRDRAMQRLL DTNPEINQSD SQDSRVAPRL DRKKRTINRE ELLKQAESVM QDLGSSRAML
EIQYENEVGT GLGPTLEFYA LVSQELQRAD LGLWRGEEVA LSNPKGSQEG TKYMFSSRGL
FAVPFGRTTK PAHIAKIKMK FRFLGKLMAK AIMDFRLLDL PLGLPFYKWM LRHETSISSH
DLVNIDPGVA KSIQHLEDII RQKKRLEQDR SQTRETLQQA LESLNMNGCS VEDLGLDFTL
PGFPNIELKK GGKDIPVTIY NLEEYLRLVV YWTMNEGVSR QFESFREGFE SVFPLHHLQY
FYPEELDQLL CGSKSETWDV KTLMECCRPD HGYTHDSRAV RFLFDVLSSF DAEQQRLFLQ
FVTGSPRLPV GGFRSLNPPL TIVRKTFEST ENPDDFLPSV MTCVNYLKLP DYSSIEIMRK
KLLIAAREGQ QSFHLS
//
