ID A0A1S3TGW9_VIGRR Unreviewed; 487 AA.
AC A0A1S3TGW9;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 2.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=O-acyltransferase WSD1 {ECO:0000313|RefSeq:XP_014493014.2};
GN Name=LOC106755381 {ECO:0000313|RefSeq:XP_014493014.2};
OS Vigna radiata var. radiata (Mung bean) (Phaseolus aureus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX NCBI_TaxID=3916 {ECO:0000313|Proteomes:UP000087766, ECO:0000313|RefSeq:XP_014493014.2};
RN [1] {ECO:0000313|RefSeq:XP_014493014.2}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_014493014.2};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC Evidence={ECO:0000256|ARBA:ARBA00029340};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004162};
CC Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004162}. Membrane
CC {ECO:0000256|ARBA:ARBA00004167}; Single-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004167}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the long-chain O-
CC acyltransferase family. {ECO:0000256|ARBA:ARBA00024360}.
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DR RefSeq; XP_014493014.2; XM_014637528.2.
DR AlphaFoldDB; A0A1S3TGW9; -.
DR STRING; 3916.A0A1S3TGW9; -.
DR KEGG; vra:106755381; -.
DR OrthoDB; 2957273at2759; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000087766; Unplaced.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR PANTHER; PTHR31650:SF27; O-ACYLTRANSFERASE WSD1-LIKE PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Reference proteome {ECO:0000313|Proteomes:UP000087766};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 91..275
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 328..473
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 487 AA; 55189 MW; E69E3750E328C65B CRC64;
MVWFGLASKN NIFSSLTISM DQLYEEVEEP VSPAGQYFNS SFICSYVFGF LEMEIPIDDS
KTLPLIKDVF LPINPRFSSI MIRDENGKRK WKRVEVKPEE HVKIPKFPES ELYDEYFDEY
VTKILTERTP QNKPLWEIHI IKYPTSKAAG TVIFKLHHAL GDGYSLMGAL LSCLERIDDP
SLPLTFPSRA SSTSQASKKN VIQKLPSFIS SFFSSMLDFG SSLVKTRMAV DDKTPIRSGY
EGTESMPFAL SSISLSLDHV KAIKSKLGVT INDVITGMIF YGIRLYMEEI EYMARKAKST
AVVMLNTRNV KGYQSVKEMQ NSKVKGLWGN KISFLQVPIP KLSQSKSYKP LEFVWNARKL
IKKKRRSFSV YLIGLLLDLE MKLKGPEAVA KIIYNTLGNC SVVISNIFGP LEQMAVANHP
ISGLYFTMTG GPENIDITIM SYVKVLRITL RTLKGFIDEQ KFKFCIEKAF DVIFKAAMEI
SQTPNNK
//
