ID A0A1S3V8Q6_VIGRR Unreviewed; 436 AA.
AC A0A1S3V8Q6;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Malate dehydrogenase [NADP], chloroplastic {ECO:0000256|ARBA:ARBA00016572};
DE EC=1.1.1.82 {ECO:0000256|ARBA:ARBA00013132};
DE AltName: Full=NADP-MDH {ECO:0000256|ARBA:ARBA00033185};
GN Name=LOC106772560 {ECO:0000313|RefSeq:XP_014514534.1};
OS Vigna radiata var. radiata (Mung bean) (Phaseolus aureus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Vigna.
OX NCBI_TaxID=3916 {ECO:0000313|Proteomes:UP000087766, ECO:0000313|RefSeq:XP_014514534.1};
RN [1] {ECO:0000313|RefSeq:XP_014514534.1}
RP IDENTIFICATION.
RC TISSUE=Leaf {ECO:0000313|RefSeq:XP_014514534.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-malate + NADP(+) = H(+) + NADPH + oxaloacetate;
CC Xref=Rhea:RHEA:10824, ChEBI:CHEBI:15378, ChEBI:CHEBI:15589,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.82;
CC Evidence={ECO:0000256|ARBA:ARBA00001500};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000256|ARBA:ARBA00004229}.
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 2 family.
CC {ECO:0000256|ARBA:ARBA00009613}.
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DR RefSeq; XP_014514534.1; XM_014659048.2.
DR AlphaFoldDB; A0A1S3V8Q6; -.
DR STRING; 3916.A0A1S3V8Q6; -.
DR GeneID; 106772560; -.
DR KEGG; vra:106772560; -.
DR OrthoDB; 312003at2759; -.
DR Proteomes; UP000087766; Chromosome 8.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0046554; F:malate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR CDD; cd01338; MDH_choloroplast_like; 1.
DR Gene3D; 3.90.110.10; Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR022383; Lactate/malate_DH_C.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR001252; Malate_DH_AS.
DR InterPro; IPR011273; Malate_DH_NADP-dep_pln.
DR InterPro; IPR010945; Malate_DH_type2.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR01757; Malate-DH_plant; 1.
DR NCBIfam; TIGR01759; MalateDH-SF1; 1.
DR PANTHER; PTHR23382; MALATE DEHYDROGENASE; 1.
DR PANTHER; PTHR23382:SF0; MALATE DEHYDROGENASE 2, MITOCHONDRIAL-RELATED; 1.
DR Pfam; PF02866; Ldh_1_C; 1.
DR Pfam; PF00056; Ldh_1_N; 1.
DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00068; MDH; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Reference proteome {ECO:0000313|Proteomes:UP000087766};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DOMAIN 95..242
FT /note="Lactate/malate dehydrogenase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00056"
FT DOMAIN 245..415
FT /note="Lactate/malate dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02866"
SQ SEQUENCE 436 AA; 47770 MW; 4FBFB2C9DC3F32E6 CRC64;
MAVTHFNPTC SKPRLHSSQL SFLSRTLPRH RHCSFAPLHR TQHARISCSV APNEVQVPAV
TTLDPKSKAD CYGVFCLTYD LRAEEETRSW KKLINIAVSG AAGMIANHLL FKLASGEVFG
PDQPIALKLL GSERSIQALE GVAMELEDSL FPLLREVSIG IDPYEVFQDA EWALLIGAKP
RGPGMERADL LDINGQIYAA QGRALNAVAS RNVKVIVVGN PCNTNALICL KNAPNIPAKN
FHALTRLDEN RAKCQLALKA GVFYDKVSNV TIWGNHSTTQ VPDFLNAKID GLPVKEVVKD
IRWLEEEFTE KVQKRGGALI QKWGRSSAAS TSVSIVDAIR SLVTPTPEGD WFSSGVYSNG
NPYGIAEGIV FSMPCRSKGD GDYELVKDVI FDDYLRQRIK KTEAELLAEK RCVAHLTGEG
IAVCDLPGDT MLPGEM
//
