ID A0A1S3WTJ2_ERIEU Unreviewed; 448 AA.
AC A0A1S3WTJ2;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 1 isoform X3 {ECO:0000313|RefSeq:XP_016049650.1};
GN Name=PFKFB1 {ECO:0000313|RefSeq:XP_016049650.1};
OS Erinaceus europaeus (Western European hedgehog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Eulipotyphla; Erinaceidae; Erinaceinae;
OC Erinaceus.
OX NCBI_TaxID=9365 {ECO:0000313|Proteomes:UP000079721, ECO:0000313|RefSeq:XP_016049650.1};
RN [1] {ECO:0000313|RefSeq:XP_016049650.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Synthesis and degradation of fructose 2,6-bisphosphate.
CC {ECO:0000256|ARBA:ARBA00003771}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the phosphoglycerate
CC mutase family. {ECO:0000256|ARBA:ARBA00008408}.
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DR RefSeq; XP_016049650.1; XM_016194164.1.
DR AlphaFoldDB; A0A1S3WTJ2; -.
DR STRING; 9365.ENSEEUP00000011485; -.
DR GeneID; 103125956; -.
DR CTD; 5207; -.
DR OrthoDB; 2013830at2759; -.
DR Proteomes; UP000079721; Unplaced.
DR GO; GO:0003873; F:6-phosphofructo-2-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004331; F:fructose-2,6-bisphosphate 2-phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0006003; P:fructose 2,6-bisphosphate metabolic process; IEA:InterPro.
DR GO; GO:0006000; P:fructose metabolic process; IEA:InterPro.
DR CDD; cd07067; HP_PGM_like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 3.40.50.1240; Phosphoglycerate mutase-like; 1.
DR InterPro; IPR003094; 6Pfruct_kin.
DR InterPro; IPR013079; 6Phosfructo_kin.
DR InterPro; IPR013078; His_Pase_superF_clade-1.
DR InterPro; IPR029033; His_PPase_superfam.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001345; PG/BPGM_mutase_AS.
DR PANTHER; PTHR10606; 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE; 1.
DR PANTHER; PTHR10606:SF15; 6-PHOSPHOFRUCTO-2-KINASE_FRUCTOSE-2,6-BISPHOSPHATASE 1; 1.
DR Pfam; PF01591; 6PF2K; 1.
DR Pfam; PF00300; His_Phos_1; 1.
DR PIRSF; PIRSF000709; 6PFK_2-Ptase; 1.
DR PRINTS; PR00991; 6PFRUCTKNASE.
DR SMART; SM00855; PGAM; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF53254; Phosphoglycerate mutase-like; 1.
DR PROSITE; PS00175; PG_MUTASE; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000079721};
KW Transferase {ECO:0000256|ARBA:ARBA00022777}.
FT DOMAIN 8..228
FT /note="6-phosphofructo-2-kinase"
FT /evidence="ECO:0000259|Pfam:PF01591"
FT ACT_SITE 236
FT /note="Tele-phosphohistidine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR613078-1"
FT ACT_SITE 305
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR613078-1"
FT BINDING 235..242
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR613078-2"
FT BINDING 285
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR613078-2"
SQ SEQUENCE 448 AA; 52038 MW; 6BAFDF492A14EEE9 CRC64;
MEEKVSRRKA SIPQFTNSPT MVIMVGLPAR GKTYISTKLT RYLNWIGTPT KVFNLGQYRR
EAVNYKNYEF FLPDNMEALH IRKQCALAAL KDVHDFLSQG EGHVAVFDAT NTTRERRSMI
LQFAKQHSYK AFFIESICND PGIIAENIKQ VKLSSPDYID CDQEKVLEDF LKRIECYATN
YQPLDDELDS HLSYIKIFDV GMRYLVNRLQ DHIQSRTVYY LMNIHVTPRS IYLCRHGESE
LNLRGRIGGD SGLSARGKQY SYALANFIQS QDINSLKVWT SHMKRTIQTA EALGVPYEQW
KALNEIDAGV CEEMTYEEIQ EHYPEEFALR DQDKYRYRYP KGESYEDLVQ RLEPVIMELE
RQENVLVICH QAVMRCLLAY FLDKNSGELP YLKCPLHTVL KLTPVAYGCK VESIYLNVEA
INTHREKPEN VDITRESEEA LDTVPAHY
//
