ID A0A1S3WVX0_ERIEU Unreviewed; 346 AA.
AC A0A1S3WVX0;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Acetylserotonin O-methyltransferase {ECO:0000256|ARBA:ARBA00040730};
DE EC=2.1.1.4 {ECO:0000256|ARBA:ARBA00039116};
DE AltName: Full=Hydroxyindole O-methyltransferase {ECO:0000256|ARBA:ARBA00043054};
GN Name=ASMT {ECO:0000313|RefSeq:XP_016050523.1};
OS Erinaceus europaeus (Western European hedgehog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Eulipotyphla; Erinaceidae; Erinaceinae;
OC Erinaceus.
OX NCBI_TaxID=9365 {ECO:0000313|Proteomes:UP000079721, ECO:0000313|RefSeq:XP_016050523.1};
RN [1] {ECO:0000313|RefSeq:XP_016050523.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the transfer of a methyl group onto N-
CC acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).
CC {ECO:0000256|ARBA:ARBA00037645}.
CC -!- PATHWAY: Aromatic compound metabolism; melatonin biosynthesis;
CC melatonin from serotonin: step 1/2. {ECO:0000256|ARBA:ARBA00037926}.
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DR RefSeq; XP_016050523.1; XM_016195037.1.
DR AlphaFoldDB; A0A1S3WVX0; -.
DR CTD; 438; -.
DR InParanoid; A0A1S3WVX0; -.
DR OrthoDB; 5230501at2759; -.
DR Proteomes; UP000079721; Unplaced.
DR GO; GO:0008171; F:O-methyltransferase activity; IEA:InterPro.
DR GO; GO:0030187; P:melatonin biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR031725; ASMT_dimerisation.
DR InterPro; IPR016461; COMT-like.
DR InterPro; IPR001077; O_MeTrfase_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11746:SF327; ACETYLSEROTONIN O-METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11746; O-METHYLTRANSFERASE; 1.
DR Pfam; PF16864; Dimerisation2; 1.
DR Pfam; PF00891; Methyltransf_2; 1.
DR PIRSF; PIRSF005739; O-mtase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51683; SAM_OMT_II; 1.
PE 4: Predicted;
KW Melatonin biosynthesis {ECO:0000256|ARBA:ARBA00043260};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000079721};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022603}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..346
FT /note="Acetylserotonin O-methyltransferase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5010277473"
FT DOMAIN 8..82
FT /note="Acetylserotonin O-methyltransferase dimerisation"
FT /evidence="ECO:0000259|Pfam:PF16864"
FT DOMAIN 100..328
FT /note="O-methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF00891"
FT ACT_SITE 238
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005739-1"
SQ SEQUENCE 346 AA; 37206 MW; EFBCCB89F913C4DA CRC64;
MSDKRKLVLF AACELGVFDL LAEAGPLDAI GVAARLDTSP QATETLLDTC VSLRLLTRDA
TVSPAAYGST RLSRTFLARG SPLSQTHLLS YTATTVYRTW AHLASAVREG RNQHLRAFGA
SSRDLFSAIY RSEEERLLFL RALQQTWGVH GPPTLTAFDL SPFARICDLG GGLGGLARQC
AALYPNSRVT VLDLPEVVAV ARTHCPLPQD ARVNFLEGDF LRDPLPVADL FILARILHDW
TDDRCRHLLA RIPGFPVPLS RPPVVSPSPD GPGARAGGGV LVVESLLDAD GRGPLATQLL
SVNMLLQTEG RERSAGEYHA LLAAAGFSHL QVRSGLGLYA ALLATQ
//
