ID A0A1S3X7C3_TOBAC Unreviewed; 729 AA.
AC A0A1S3X7C3;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
GN Name=LOC107762105 {ECO:0000313|RefSeq:XP_016435915.1};
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097 {ECO:0000313|Proteomes:UP000084051, ECO:0000313|RefSeq:XP_016435915.1};
RN [1] {ECO:0000313|Proteomes:UP000084051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TN90 {ECO:0000313|Proteomes:UP000084051};
RX PubMed=24807620; DOI=10.1038/ncomms4833;
RA Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A.,
RA Goepfert S., Peitsch M.C., Ivanov N.V.;
RT "The tobacco genome sequence and its comparison with those of tomato and
RT potato.";
RL Nat. Commun. 5:3833-3833(2014).
RN [2] {ECO:0000313|RefSeq:XP_016435915.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR RefSeq; XP_016435915.1; XM_016580429.1.
DR AlphaFoldDB; A0A1S3X7C3; -.
DR SMR; A0A1S3X7C3; -.
DR STRING; 4097.A0A1S3X7C3; -.
DR PaxDb; 4097-A0A1S3X7C3; -.
DR GeneID; 107762105; -.
DR KEGG; nta:107762105; -.
DR OMA; PEFHTAK; -.
DR OrthoDB; 5489808at2759; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF51; BETA-GALACTOSIDASE 5; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000084051};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..26
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 27..729
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5010262918"
FT DOMAIN 35..339
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 362..418
FT /note="Beta-galactosidase beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF17834"
FT DOMAIN 622..706
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
SQ SEQUENCE 729 AA; 81318 MW; 9891DF33994EA0B0 CRC64;
METSSISKFF IVYAFSLLMV AQLICCKVTY DKKAIIINGQ RRILLSGSIH YPRSTPEMWE
DLIKKAKDAN LDVIETYVFW NIHEPSPGNY DFEGRYDLVR FIKTVQKAGL YAHLRIGPYV
CAEWNFGGFP VWLKYVPGIS FRTDNEPFKA AMQKFTQKIV EMMKSEKLFE SQGGPIILSQ
IENEYEPARK ALGTAGEAYV QWAAKMAVGL DTGVPWVMCK EDDAPDPIIN TCNGFYCDEF
SPNKPYKPTM WTEAWSGWFT EFGGTIPMRP VQDLAFAVAR FIQKGGSFVN YYMFHGGTNF
GRTAGGPFIT TSYDYDAPID EYGLIREPKY SHLKELHRAI KLCERALISS DPNVISLGSS
QEAHVFSSGK RNCAAFLSNY DSNSAARVVF NNKHFNLPPW SISILPDCTH VAFNTAKVGT
RTSQVKMIPT GSQLHSWGAY SEDVFSLEDS STFAANGLLE QINITRDNSD YLWYITSVDI
SPSESFLRGG QKPTLSVHSS GHAVHVFVNG KLSGSSYGSR RDTKFTFTGP VGLQAGTNRI
ELLSIAVGLP NIGLHYEEWN TGILGPVVIQ GLNQGHKDIS SQKWTYKVGL KGEAMNLISH
HGVSSVDWIG GALATQGQQP LKWYKAYFDA PEGNDPVALD MRSMGKGQVW INGQSIGRYW
TASANGNCDA FHYAGTYRQT KCQLGCGQPT QKWYHVPRSW LKPTQNLLVL FEELGGDVSK
ILLVKRTTS
//
