ID A0A1S3XVI4_TOBAC Unreviewed; 466 AA.
AC A0A1S3XVI4;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=5-epiaristolochene synthase {ECO:0000256|ARBA:ARBA00024390};
DE EC=4.2.3.61 {ECO:0000256|ARBA:ARBA00024390};
GN Name=LOC107769201 {ECO:0000313|RefSeq:XP_016443889.1};
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097 {ECO:0000313|Proteomes:UP000084051, ECO:0000313|RefSeq:XP_016443889.1};
RN [1] {ECO:0000313|Proteomes:UP000084051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TN90 {ECO:0000313|Proteomes:UP000084051};
RX PubMed=24807620; DOI=10.1038/ncomms4833;
RA Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A.,
RA Goepfert S., Peitsch M.C., Ivanov N.V.;
RT "The tobacco genome sequence and its comparison with those of tomato and
RT potato.";
RL Nat. Commun. 5:3833-3833(2014).
RN [2] {ECO:0000313|RefSeq:XP_016443889.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004721}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the terpene synthase family.
CC {ECO:0000256|ARBA:ARBA00006333}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR RefSeq; XP_016443889.1; XM_016588403.1.
DR AlphaFoldDB; A0A1S3XVI4; -.
DR GeneID; 107769201; -.
DR OrthoDB; 723931at2759; -.
DR UniPathway; UPA00213; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.10.600.10; Farnesyl Diphosphate Synthase; 2.
DR Gene3D; 1.50.10.130; Terpene synthase, N-terminal domain; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR31225:SF125; 5-EPI-ARISTOLOCHENE SYNTHASE; 1.
DR PANTHER; PTHR31225; OS04G0344100 PROTEIN-RELATED; 1.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 2.
DR SFLD; SFLDS00005; Isoprenoid_Synthase_Type_I; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SFLD; SFLDG01014; Terpene_Cyclase_Like_1_N-term; 1.
DR SUPFAM; SSF48239; Terpenoid cyclases/Protein prenyltransferases; 1.
DR SUPFAM; SSF48576; Terpenoid synthases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000084051}.
FT DOMAIN 24..194
FT /note="Terpene synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF01397"
FT DOMAIN 252..367
FT /note="Terpene synthase metal-binding"
FT /evidence="ECO:0000259|Pfam:PF03936"
FT DOMAIN 369..408
FT /note="Terpene synthase metal-binding"
FT /evidence="ECO:0000259|Pfam:PF03936"
SQ SEQUENCE 466 AA; 53551 MW; 972E52C6C841B646 CRC64;
MAAAAVGNYE EEIVRPVADF SPSLWGDQFL SFSIKNQVAE KYAKEIEALK EQTRNMLLAT
GMKLADTLNL IDIIERLGIS YHFEKEIDEI LDQIYNQNSN CDDLCISALQ FRLLRQHGFN
ISPEIFSKFQ DENGKFKESL ASDVLGLLNL YEASHVRTHA DDILEDALAF STIHLESAAP
HLKSPLKEQV THALEQCLHK GVPRVETRFF ISSIYDKEQS KNNVLLRFAK LDFNLLQMLH
KQELAQVSRW WKDLDFVTTL PYARDRVVEC YFWALGVYFE PQYSQARVML VKTISMISIV
DDTFDAYGTV KELEAFTDAI QRWDINEIDR LPDYMKISYK AILDLYKDYE KELSSAGRSH
IVCHAIERVE KSRGQIATGI ECCMRDYGIT TKEAMTKFQE MAEAAWKDLN EGLLRPTPVA
TEFLSRILNL ARIVEVTYIH NLDGYTHPEK VLKPHINALL VDSIEI
//
