ID A0A1S3ZN21_TOBAC Unreviewed; 844 AA.
AC A0A1S3ZN21;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
GN Name=LOC107788590 {ECO:0000313|RefSeq:XP_016465757.1};
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097 {ECO:0000313|Proteomes:UP000084051, ECO:0000313|RefSeq:XP_016465757.1};
RN [1] {ECO:0000313|Proteomes:UP000084051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TN90 {ECO:0000313|Proteomes:UP000084051};
RX PubMed=24807620; DOI=10.1038/ncomms4833;
RA Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A.,
RA Goepfert S., Peitsch M.C., Ivanov N.V.;
RT "The tobacco genome sequence and its comparison with those of tomato and
RT potato.";
RL Nat. Commun. 5:3833-3833(2014).
RN [2] {ECO:0000313|RefSeq:XP_016465757.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
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DR RefSeq; XP_016465757.1; XM_016610271.1.
DR AlphaFoldDB; A0A1S3ZN21; -.
DR SMR; A0A1S3ZN21; -.
DR STRING; 4097.A0A1S3ZN21; -.
DR PaxDb; 4097-A0A1S3ZN21; -.
DR GeneID; 107788590; -.
DR KEGG; nta:107788590; -.
DR OMA; APEMWPD; -.
DR OrthoDB; 5489808at2759; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0004565; F:beta-galactosidase activity; IBA:GO_Central.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd22842; Gal_Rha_Lectin_BGal; 1.
DR Gene3D; 2.60.120.740; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR000922; Lectin_gal-bd_dom.
DR InterPro; IPR043159; Lectin_gal-bd_sf.
DR PANTHER; PTHR23421:SF74; BETA-GALACTOSIDASE 1; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 2.
DR Pfam; PF02140; Gal_Lectin; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
DR PROSITE; PS50228; SUEL_LECTIN; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000084051};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..31
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 32..844
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5010179082"
FT DOMAIN 758..844
FT /note="SUEL-type lectin"
FT /evidence="ECO:0000259|PROSITE:PS50228"
SQ SEQUENCE 844 AA; 93610 MW; 755DEE75E9817670 CRC64;
MLINMVSRLV MSNVLLMLLV LLGSCVFSGL ASVSYDRNAI IVNGQKRILI SGSIHYPRST
PEMWPDLIQK AKKGGIDVIQ TYVFWNGHEP EQGKYYFEER YDLVKFIKLV HQAGLYVHLR
VGPYACAEWN FGGFPVWLKY VPGISFRTDN GPFKAAMQKF TTKIVNMMKA ERLYESQGGP
IILSQIENEY GPIELRLGEA GKSYSEWAAK MALDLGTGVP WVMCKQNDAP DPVINTCNGF
YCDTFSPNKA YKPKMWTEAW TAWFTEFGGP VPYRPVEDLA FGVAKFIQTG GSFINYYMYH
GGTNFGRTAG GPFIATSYDY DAPLDEFGLL RQPKWGHLKD LHRAIKLCEP ALVSGDPTVT
SLGHYQEARV FRSKSGACAA FLANYNQHSF ATVAFANRHY NLPPWSISIL PDCKNTVYNT
ARVGAQSALM KMTPVNRGFS WQSYNDEPAS YEDNTFTVVG LLEQINTTRD VSDYLWYMTD
VKIDPREGFL SSGKWPWLRV SSAGPALHVF VNDQLAGTVY GSLKSQKLTF NKAVNLRAGV
NKISLLSIAV GLPNIGPHFE TWNTGVLGPV SLSGLDEGKR DLTWQKWSYK VGLKGEALNL
HSLSGSSSVE WVEGSLVAQR QPLTWLKTTF NAPAGNEPLA LDMNAMGKGQ VWINGQSLGR
YWPGYKASGT CGACNYAGFF SEDKCQSNCG EASQRWYHVP RSWLHPTGNL LVVFEEWGGD
PNAISLVKRE LASVCADINE WQPQLVNWQM QASGKVDKPL RPRAHLSCAP GQKITSIKFA
SFGSPQGVCG SFREGSCHAH HSYDAFEKYC IGQESCTVPV TPEIFGGDPC PGVMKKLSVE
AICS
//
