ID A0A1S4B945_TOBAC Unreviewed; 501 AA.
AC A0A1S4B945;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 {ECO:0000313|RefSeq:XP_016485318.1};
GN Name=LOC107805758 {ECO:0000313|RefSeq:XP_016485318.1};
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097 {ECO:0000313|Proteomes:UP000084051, ECO:0000313|RefSeq:XP_016485318.1};
RN [1] {ECO:0000313|Proteomes:UP000084051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TN90 {ECO:0000313|Proteomes:UP000084051};
RX PubMed=24807620; DOI=10.1038/ncomms4833;
RA Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A.,
RA Goepfert S., Peitsch M.C., Ivanov N.V.;
RT "The tobacco genome sequence and its comparison with those of tomato and
RT potato.";
RL Nat. Commun. 5:3833-3833(2014).
RN [2] {ECO:0000313|RefSeq:XP_016485318.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family.
CC {ECO:0000256|ARBA:ARBA00010617, ECO:0000256|RuleBase:RU000461}.
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DR RefSeq; XP_016485318.1; XM_016629832.1.
DR AlphaFoldDB; A0A1S4B945; -.
DR SMR; A0A1S4B945; -.
DR STRING; 4097.A0A1S4B945; -.
DR PaxDb; 4097-A0A1S4B945; -.
DR GeneID; 107805758; -.
DR KEGG; nta:107805758; -.
DR OMA; RFVSHPI; -.
DR OrthoDB; 1103245at2759; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR PANTHER; PTHR47950:SF37; (S)-N-METHYLCOCLAURINE 3'-HYDROXYLASE ISOZYME 2; 1.
DR PANTHER; PTHR47950; CYTOCHROME P450, FAMILY 76, SUBFAMILY C, POLYPEPTIDE 5-RELATED; 1.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; Cytochrome P450; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 3: Inferred from homology;
KW Heme {ECO:0000256|RuleBase:RU000461}; Iron {ECO:0000256|RuleBase:RU000461};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|RuleBase:RU000461};
KW Monooxygenase {ECO:0000256|RuleBase:RU000461};
KW Oxidoreductase {ECO:0000256|RuleBase:RU000461};
KW Reference proteome {ECO:0000313|Proteomes:UP000084051};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 6..23
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 170..190
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
SQ SEQUENCE 501 AA; 56027 MW; D8B80157CB64865E CRC64;
MIDYIFTSIL VSLVFVIMLK YLYSSKTKLP LPPGPFPLPL VGNLFQVGRK RPHAALAKLA
QSHGRDLMSI RFGTRLVVVA SSPNAASEVL KTHDRMLSGR FVSHPIRVKG SLLHNVSTAF
LEECDDNWKS VRTVYRGALF STKALESQVS LREKKVKEMM QHLVTKQGKV ISIKGVVFVT
ALNILGNLLL SIDLIDFEGK GVGEELREYL RKFTAAGGIL ELADLYPILG VCSSDVQGTY
KKLMGLFDKL CAVWAGIIQD RRNKSENQPS ADAVDFIDAL VKNGFSDKHI NALLVEMFGA
GTESTTATSE WMLVELLRNQ QALQKLRHEI AGVVGGKDFV KESDLSTLPY LDACFKETLR
LHPPGPLLLP HRAVQTCEVM GYRIPKDTQV LVNMWAIARD PKIWDDASSF KPERFINAKI
DHKGRNFEYI PFGSGRRICA GEPLASRFIP LAVASFIHKF DWILPNDMDP AHISMDEILD
ITMFKKDPLL VIPKLRKRGE D
//
