ID A0A1S4CYF1_TOBAC Unreviewed; 669 AA.
AC A0A1S4CYF1;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=tRNA(Ile)-lysidine synthetase {ECO:0000256|ARBA:ARBA00013267};
DE EC=6.3.4.19 {ECO:0000256|ARBA:ARBA00013267};
GN Name=LOC107823950 {ECO:0000313|RefSeq:XP_016506156.1};
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097 {ECO:0000313|Proteomes:UP000084051, ECO:0000313|RefSeq:XP_016506156.1};
RN [1] {ECO:0000313|Proteomes:UP000084051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TN90 {ECO:0000313|Proteomes:UP000084051};
RX PubMed=24807620; DOI=10.1038/ncomms4833;
RA Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A.,
RA Goepfert S., Peitsch M.C., Ivanov N.V.;
RT "The tobacco genome sequence and its comparison with those of tomato and
RT potato.";
RL Nat. Commun. 5:3833-3833(2014).
RN [2] {ECO:0000313|RefSeq:XP_016506156.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP +
CC diphosphate + H(+) + lysidine(34) in tRNA(Ile2);
CC Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665,
CC ChEBI:CHEBI:456215; EC=6.3.4.19;
CC Evidence={ECO:0000256|ARBA:ARBA00000047};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR RefSeq; XP_016506156.1; XM_016650670.1.
DR AlphaFoldDB; A0A1S4CYF1; -.
DR GeneID; 107823950; -.
DR OMA; RFLIRWD; -.
DR OrthoDB; 460053at2759; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR CDD; cd01992; PP-ATPase; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_01161; tRNA_Ile_lys_synt; 1.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR011063; TilS/TtcA_N.
DR InterPro; IPR012094; tRNA_Ile_lys_synt.
DR InterPro; IPR012795; tRNA_Ile_lys_synt_N.
DR NCBIfam; TIGR02432; lysidine_TilS_N; 1.
DR PANTHER; PTHR43033; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR PANTHER; PTHR43033:SF1; TRNA(ILE)-LYSIDINE SYNTHASE-RELATED; 1.
DR Pfam; PF01171; ATP_bind_3; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Reference proteome {ECO:0000313|Proteomes:UP000084051}.
FT DOMAIN 54..258
FT /note="tRNA(Ile)-lysidine/2-thiocytidine synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01171"
SQ SEQUENCE 669 AA; 74083 MW; 5416278711805BE8 CRC64;
MSMARGFFVC PQTKNSSHRC STHRQENVPI DMSAYSDTFA KRMAMAGLKP HHRIVLGVSG
GPDSMALCVL TAAWKTNSLG IAAQKNEFID GLLAVVVDHG LRAESKDEAN LVHRRVTSMG
IKCEIACCEW SEGKPKQGHL QEAAREKRYE ILQSASIRHQ IGVLMTAHHA DDQAELFILR
LSRNSGVLGL AGMAFVSQLF CTCPDLSAEA SGEGILLVRP LLEFSKEDMY KICLGANQEW
VEDPTNRSAL FTRNRIRMTL TDLASPIFRS ELQALIAACR RTRSHVDKMC SNLLHQAVTI
MPEGYAVIDL GILRPSDLKD IVLSKFIALL LQFVSQRQRP VRGRASKLVL NYIRTSPCKT
AVTASGCYLC PAPGSRGTQV LVCRSTDADL TLEFLNPYLT EGKNCSISNE VKQIVANGRS
YSDQFPQSTL AMQFLDLTSS DSILAEAKRE GILSESTYKS IISLQREESN NFVSKTNIVL
ENKVEHKVEY AASAPSKVLR PEKVGYFMNR FIVKWNLSKQ NACTSFSTNN SNQFQDFREE
WNFCNSCVVG YDRLASVRYM VDADWLYLAT LSKREDSCSV HEELSLSVES RVMSNINLCS
ACTKKSAERA LLLLKSIPVA ARRALPVLVN VDGVLLSIPS VGFQHCPCLV ASATYKPKIP
LGGGYNSFL
//
