ID A0A1S4DP88_TOBAC Unreviewed; 575 AA.
AC A0A1S4DP88;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=rhamnogalacturonan endolyase {ECO:0000256|ARBA:ARBA00012437};
DE EC=4.2.2.23 {ECO:0000256|ARBA:ARBA00012437};
GN Name=LOC107831685 {ECO:0000313|RefSeq:XP_016514954.1};
OS Nicotiana tabacum (Common tobacco).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097 {ECO:0000313|Proteomes:UP000084051, ECO:0000313|RefSeq:XP_016514954.1};
RN [1] {ECO:0000313|Proteomes:UP000084051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TN90 {ECO:0000313|Proteomes:UP000084051};
RX PubMed=24807620; DOI=10.1038/ncomms4833;
RA Sierro N., Battey J.N., Ouadi S., Bakaher N., Bovet L., Willig A.,
RA Goepfert S., Peitsch M.C., Ivanov N.V.;
RT "The tobacco genome sequence and its comparison with those of tomato and
RT potato.";
RL Nat. Commun. 5:3833-3833(2014).
RN [2] {ECO:0000313|RefSeq:XP_016514954.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-
CC (1->4)-alpha-D-galactopyranosyluronic acid bonds of
CC rhamnogalacturonan I domains in ramified hairy regions of pectin
CC leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-
CC unsaturated D-galactopyranosyluronic acid at the non-reducing end.;
CC EC=4.2.2.23; Evidence={ECO:0000256|ARBA:ARBA00001324};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 4 family.
CC {ECO:0000256|ARBA:ARBA00010418}.
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DR RefSeq; XP_016514954.1; XM_016659468.1.
DR AlphaFoldDB; A0A1S4DP88; -.
DR STRING; 4097.A0A1S4DP88; -.
DR PaxDb; 4097-A0A1S4DP88; -.
DR GeneID; 107831685; -.
DR KEGG; nta:107831685; -.
DR OMA; DCAANET; -.
DR OrthoDB; 1215403at2759; -.
DR Proteomes; UP000084051; Unplaced.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0102210; F:rhamnogalacturonan endolyase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR CDD; cd10317; RGL4_C; 1.
DR CDD; cd10316; RGL4_M; 1.
DR CDD; cd10320; RGL4_N; 1.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.40.1120; Carboxypeptidase-like, regulatory domain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR029413; RG-lyase_II.
DR InterPro; IPR029411; RG-lyase_III.
DR InterPro; IPR010325; Rhamnogal_lyase.
DR PANTHER; PTHR32018:SF39; OS11G0134100 PROTEIN; 1.
DR PANTHER; PTHR32018; RHAMNOGALACTURONATE LYASE FAMILY PROTEIN; 1.
DR Pfam; PF14683; CBM-like; 1.
DR Pfam; PF14686; fn3_3; 1.
DR Pfam; PF06045; Rhamnogal_lyase; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 1.
PE 3: Inferred from homology;
KW Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|RefSeq:XP_016514954.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000084051};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 294..365
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14686"
FT DOMAIN 380..568
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14683"
SQ SEQUENCE 575 AA; 65950 MW; AC7D39ABF31AE5B2 CRC64;
MNSYLLDALF NVLICTTYKV ILERDDQIEL SFSRTWDVSL QDKLIPLNID KRFIMLKGSS
GFYSYAIYEH LEVWPGFNLD ETRIAFKLRK DKFHYMAMAD NRQRYMPLPD DRLPDRGQPL
AYPEAVLLVN PVEPELKGEV DDKYQYSSED KDLKVHGWIC MDPPLGFWII IPSDEFRSGG
PLKQNLTSHV GPTALSVFLS AHYAGEDLVP KFGEGESWKK IFGPVFIYLN SVTGGKDPLI
LWEDAKEQML VEVQSWPYSF PASEDFPSSS QRGNISGRLL VQDRYFKDDK IYASGAYVGL
APPGEAGSWQ RECKDYQFWT KADEEGYYVI SDIRAGDYNL YAFVPGFIGD YKSDTKVTIT
SGCSIEMDDL VFEPPRNGPT LWEIGIPDRS AREFYIPDPD PKYINKLFVN HPDKFRQYGL
WERYTDLYPN GDIVFTIGES DYKKDWFFAQ VTRKKDEKTY TGTTWQIKFK LDSVNRNETY
SLRLALASVA QAELQVRIND SSMNTPLFSS GLIGKDNAIA RHGIHGLYWL FNVNLQGDML
VEGENTIYLT QANATSPFQG IMYDYIRLEA PPSHD
//
