ID A0A1S4DYA0_CUCME Unreviewed; 589 AA.
AC A0A1S4DYA0;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=beta-galactosidase {ECO:0000256|ARBA:ARBA00012756};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
GN Name=LOC103492528 {ECO:0000313|RefSeq:XP_016900957.1};
OS Cucumis melo (Muskmelon).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX NCBI_TaxID=3656 {ECO:0000313|Proteomes:UP000089565, ECO:0000313|RefSeq:XP_016900957.1};
RN [1] {ECO:0000313|Proteomes:UP000089565}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. DHL92 {ECO:0000313|Proteomes:UP000089565};
RX PubMed=22753475; DOI=10.1073/pnas.1205415109;
RA Garcia-Mas J., Benjak A., Sanseverino W., Bourgeois M., Mir G.,
RA Gonzalez V.M., Henaff E., Camara F., Cozzuto L., Lowy E., Alioto T.,
RA Capella-Gutierrez S., Blanca J., Canizares J., Ziarsolo P.,
RA Gonzalez-Ibeas D., Rodriguez-Moreno L., Droege M., Du L.,
RA Alvarez-Tejado M., Lorente-Galdos B., Mele M., Yang L., Weng Y.,
RA Navarro A., Marques-Bonet T., Aranda M.A., Nuez F., Pico B., Gabaldon T.,
RA Roma G., Guigo R., Casacuberta J.M., Arus P., Puigdomenech P.;
RT "The genome of melon (Cucumis melo L.).";
RL Proc. Natl. Acad. Sci. U.S.A. 109:11872-11877(2012).
RN [2] {ECO:0000313|RefSeq:XP_016900957.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809}.
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DR RefSeq; XP_016900957.1; XM_017045468.1.
DR AlphaFoldDB; A0A1S4DYA0; -.
DR eggNOG; KOG0496; Eukaryota.
DR InParanoid; A0A1S4DYA0; -.
DR OrthoDB; 5489808at2759; -.
DR Proteomes; UP000089565; Unplaced.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF63; BETA-GALACTOSIDASE 12; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000089565};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..589
FT /note="beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5010215827"
FT DOMAIN 29..332
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 355..411
FT /note="Beta-galactosidase beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF17834"
FT DOMAIN 462..529
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT NON_TER 589
FT /evidence="ECO:0000313|RefSeq:XP_016900957.1"
SQ SEQUENCE 589 AA; 66679 MW; EB9714044CC6200B CRC64;
MPKVVLLFLS LLTWVCSTIG SVTYDHKAII INGQRRILIS GSIHYPRSTP QMWPDLIQKA
KNGGLDIIET YVFWNGHEPS PGKYYFEERY DLVRFIKLVH QAGLYVHLRI GPYVCAEWNY
GGFPVWLKFV PGIAFRTDNK PFKAAMQKFV YKIVDLMKWE KLYHTQGGPI ILSQIENEYG
PVEWEIGAPG KSYTKWAAQM ALGLKTGVPW VMCKQEDAPD PLIDTCNGFY CENFKPNKIY
KPKIWTENWS GWYTAFGGPT PYRPPEDVAF SVARFIQNGG SLVNYYMYHG GTNFGRTSGL
FIATSYDFDA PIDEYGLLRE PKWGHLRDLH KAIKSCEPAL VSADPTSTWL GKNQEARVFK
SRSGACAAFL ANYDTSASVR VNFWNHPYDL PPWSISILPD CKTVTFNTAR IGVKSYQAKM
TPISSFWWLS YKEEPASGYA SDTTTKDGLV EQVSVTWDTT DYLWYMTDIR IDSTEGFLKT
GRWPLLTVDS AGHVLHVFIN GQLSGSVYGS LEDPRITFSK YVNLKEGVNK LSMLSVTVGL
PNVGLHFDTW NAGVLGPVTL KGLNEGTRDM SKYKWSYKVG LKGEILNLY
//
