ID A0A1S6IUF8_9FIRM Unreviewed; 247 AA.
AC A0A1S6IUF8;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=CRISPR-associated endoribonuclease {ECO:0000256|PIRNR:PIRNR005054};
GN ORFNames=B0537_04425 {ECO:0000313|EMBL:AQS58400.1};
OS Desulforamulus ferrireducens.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptococcaceae;
OC Desulforamulus.
OX NCBI_TaxID=1833852 {ECO:0000313|EMBL:AQS58400.1, ECO:0000313|Proteomes:UP000189464};
RN [1] {ECO:0000313|EMBL:AQS58400.1, ECO:0000313|Proteomes:UP000189464}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GSS09 {ECO:0000313|EMBL:AQS58400.1,
RC ECO:0000313|Proteomes:UP000189464};
RX PubMed=27153808; DOI=10.1099/ijsem.0.001139;
RA Yang G., Guo J., Zhuang L., Yuan Y., Zhou S.;
RT "Desulfotomaculum ferrireducens sp. nov., a moderately thermophilic
RT sulfate-reducing and dissimilatory Fe(III)-reducing bacterium isolated from
RT compost.";
RL Int. J. Syst. Evol. Microbiol. 66:3022-3028(2016).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA).
CC {ECO:0000256|PIRNR:PIRNR005054}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC family. {ECO:0000256|ARBA:ARBA00005937, ECO:0000256|PIRNR:PIRNR005054}.
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DR EMBL; CP019698; AQS58400.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1S6IUF8; -.
DR STRING; 1833852.B0537_04425; -.
DR KEGG; dfg:B0537_04425; -.
DR OrthoDB; 9797488at2; -.
DR Proteomes; UP000189464; Chromosome.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd21140; Cas6_I-like; 1.
DR Gene3D; 3.30.70.1890; -; 1.
DR Gene3D; 3.30.70.1900; -; 1.
DR InterPro; IPR049435; Cas_Cas6_C.
DR InterPro; IPR010156; CRISPR-assoc_prot_Cas6.
DR InterPro; IPR045747; CRISPR-assoc_prot_Cas6_N_sf.
DR NCBIfam; TIGR01877; cas_cas6; 1.
DR PANTHER; PTHR36984; CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1; 1.
DR PANTHER; PTHR36984:SF1; CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1; 1.
DR Pfam; PF21350; Cas6_I-A; 1.
DR Pfam; PF01881; Cas_Cas6_C; 1.
DR PIRSF; PIRSF005054; PF1131; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW Reference proteome {ECO:0000313|Proteomes:UP000189464};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 123..242
FT /note="CRISPR associated protein Cas6 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01881"
FT ACT_SITE 28
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-50"
FT ACT_SITE 40
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-50"
FT SITE 52
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR005054-1"
SQ SEQUENCE 247 AA; 27974 MW; 680D2EA497E74314 CRC64;
MHLYIKFTSQ EDIEVPVRYN HLLQAALYET IDPPLAAFLH DKGYQSHGRS FKLFTFSRLN
GKFKMIKEKN MLRFCGDICL TVTSPVADFC HSIANRLFKL GQIRLGRQSL DVKALTTRQY
TVQSERVILR TLSPVVTYST FLRPEGRKYT CYFQPGEPDY NQLIENNLRK KYLAFYQVEA
PPGAVTVTAL SPMKLNIVNY KDTVIKGYSG KLAITGPNEL LQMAVDAGIG SKNSQGFGCV
EVVEKQI
//
