ID A0A1S8CKA5_9GAMM Unreviewed; 271 AA.
AC A0A1S8CKA5;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=2,5-didehydrogluconate reductase A {ECO:0000313|EMBL:OMQ23760.1};
GN ORFNames=BMI79_09640 {ECO:0000313|EMBL:OMQ23760.1};
OS Serratia oryzae.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Serratia.
OX NCBI_TaxID=2034155 {ECO:0000313|EMBL:OMQ23760.1, ECO:0000313|Proteomes:UP000216021};
RN [1] {ECO:0000313|EMBL:OMQ23760.1, ECO:0000313|Proteomes:UP000216021}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=J11-6 {ECO:0000313|EMBL:OMQ23760.1,
RC ECO:0000313|Proteomes:UP000216021};
RA Zhang X.-X., Zhang J.;
RT "Rahnella oryzae sp. nov., isolated from rice root.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OMQ23760.1}.
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DR EMBL; MOXD01000004; OMQ23760.1; -; Genomic_DNA.
DR RefSeq; WP_076941965.1; NZ_MOXD01000004.1.
DR AlphaFoldDB; A0A1S8CKA5; -.
DR STRING; 2034155.BMI79_09640; -.
DR OrthoDB; 9804790at2; -.
DR Proteomes; UP000216021; Unassembled WGS sequence.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0019853; P:L-ascorbic acid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR Pfam; PF00248; Aldo_ket_red; 2.
DR PIRSF; PIRSF000097; AKR; 1.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE 4: Predicted;
KW Ascorbate biosynthesis {ECO:0000256|ARBA:ARBA00022644}.
FT DOMAIN 16..190
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT DOMAIN 199..258
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT ACT_SITE 49
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT BINDING 105
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT SITE 74
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ SEQUENCE 271 AA; 30402 MW; 54637776694F035A CRC64;
MQPIIKLHDG NLMPQLGLGV WQASIAEATH AVSKALEVGY RSIDTAAIYN NEEGVGSALQ
STSLPRSELF ITTKLWNDDQ GNPQAALETS LKKLKLDYVD LYLIHWPKPA QDRYVDAWRG
LIKLREQGLA KSIGVCNFHV PHLQRLLDET GTAPVINQIE LHPMLQQRQL RAWNATHHIT
TESWSPLAQG GEGVFDQPII KRLAEKYGKT PAQIVVRWHL DSGLVVIPKS VTPARIRENF
EVFDFKLDKE ELNEIATLDI GNRLGPDPES L
//
