ID A0A1S8FFN0_9BURK Unreviewed; 385 AA.
AC A0A1S8FFN0;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=Propionate 3-nitronate monooxygenase {ECO:0000256|ARBA:ARBA00031155};
GN ORFNames=B0B52_14855 {ECO:0000313|EMBL:OOG39892.1};
OS Polaromonas sp. A23.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Polaromonas.
OX NCBI_TaxID=1944133 {ECO:0000313|EMBL:OOG39892.1, ECO:0000313|Proteomes:UP000218978};
RN [1] {ECO:0000313|EMBL:OOG39892.1, ECO:0000313|Proteomes:UP000218978}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A23 {ECO:0000313|EMBL:OOG39892.1,
RC ECO:0000313|Proteomes:UP000218978};
RA Lycus P., Bothun K.L., Bergaust L., Shapleigh J.P., Bakken L.R.,
RA Frostegard A.;
RT "Phenotypic and genotypic richness of denitrifiers revealed by a novel
RT isolation strategy.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + 3 O2 + 3 propionate 3-nitronate = 3 3-oxopropanoate + 3
CC H(+) + H2O2 + 2 nitrate + nitrite; Xref=Rhea:RHEA:57332,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:16301, ChEBI:CHEBI:17632,
CC ChEBI:CHEBI:33190, ChEBI:CHEBI:136067;
CC Evidence={ECO:0000256|ARBA:ARBA00000219};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- SIMILARITY: Belongs to the nitronate monooxygenase family. NMO class I
CC subfamily. {ECO:0000256|ARBA:ARBA00009881}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OOG39892.1}.
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DR EMBL; MUNO01000026; OOG39892.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1S8FFN0; -.
DR STRING; 1944133.B0B52_14855; -.
DR OrthoDB; 9778912at2; -.
DR Proteomes; UP000218978; Unassembled WGS sequence.
DR GO; GO:0051213; F:dioxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0018580; F:nitronate monooxygenase activity; IEA:InterPro.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR CDD; cd04730; NPD_like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR004136; NMO.
DR PANTHER; PTHR42747; NITRONATE MONOOXYGENASE-RELATED; 1.
DR PANTHER; PTHR42747:SF3; NITRONATE MONOOXYGENASE-RELATED; 1.
DR Pfam; PF03060; NMO; 1.
DR SUPFAM; SSF51412; Inosine monophosphate dehydrogenase (IMPDH); 1.
PE 3: Inferred from homology;
KW Detoxification {ECO:0000256|ARBA:ARBA00022575};
KW Dioxygenase {ECO:0000313|EMBL:OOG39892.1};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643};
KW Monooxygenase {ECO:0000256|ARBA:ARBA00023033};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000218978}.
FT DOMAIN 10..346
FT /note="Nitronate monooxygenase"
FT /evidence="ECO:0000259|Pfam:PF03060"
SQ SEQUENCE 385 AA; 40972 MW; BB437F6DB3B2FBB0 CRC64;
MSRWKTTALT QRLGLAAPIV QGPFGGGLSS VALTAAVCES GGLGSFGVHH LTGAQIEETA
QAIRLKTKRP FALNLWVPHE NSEDPPLTDP QFEQHLSLLA PYFKELGEPL PTRPERFSPR
YEDQVAGLLA ARPAVFSFVF GIPSPDILRQ CKALDIVTVG AATTPEEAMA LDSAGVDMVV
ATGFEAGGHR VSFLRPPENS LTGTLALIPQ VVDAIKAPVI AAGGIADGRG IAAALALGAQ
GVQIGTAFLA CVESSASDIH RQKLFSPDAR YTALTRAFSG RLARGIENRF MNELKAHEGG
IAPYPVQNWL TGQLKPAAIA QGRTDLMSLW SGQAAPLLRH RDARSLMQAL QQQTDDALRR
VAGSVCPSNR TCLMTTHGDR FTVEA
//
