ID A0A1S8KY73_9CLOT Unreviewed; 633 AA.
AC A0A1S8KY73;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=pcrA_3 {ECO:0000313|EMBL:OOL84674.1};
GN Synonyms=pcrA_1 {ECO:0000313|EMBL:URZ10988.1};
GN ORFNames=CROST_017040 {ECO:0000313|EMBL:URZ10988.1}, CROST_42090
GN {ECO:0000313|EMBL:OOL84674.1};
OS Clostridium felsineum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=36839 {ECO:0000313|EMBL:OOL84674.1};
RN [1] {ECO:0000313|EMBL:OOL84674.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 7320 {ECO:0000313|EMBL:OOL84674.1};
RA Poehlein A., Montoya Solano J.D., Flitsch S., Krabben P., Duerre P.,
RA Daniel R.;
RT "Microbial solvent formation.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:URZ10988.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 7320 {ECO:0000313|EMBL:URZ10988.1};
RA Poehlein A., Schoch T., Duerre P., Daniel R.;
RT "Genome sequence of C. roseum typestrain.";
RL Submitted (APR-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; LZYV01000100; OOL84674.1; -; Genomic_DNA.
DR EMBL; CP096983; URZ10988.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1S8KY73; -.
DR STRING; 84029.CROST_42090; -.
DR KEGG; crw:CROST_017040; -.
DR Proteomes; UP000190951; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 10..297
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 298..554
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 633 AA; 73606 MW; 11082BEFFAAD60D3 CRC64;
MISEEELLSG LDSDQKEAVM APLDKPSCVR ANAGSGKTRV LSSRVAYLVL KKVSGIMLLT
FTNKAAKEMT ERVQKLVGDN ISTRIISGTF HHMACIFLRR YSKAVGIDNK FSILSQSDAE
SIISRTRKEV LSEIPKNEVP KDFPTAPKIM SKYSFYKNKF KDITFDAFLR NSNVMPKSRE
LIENIIKEYE IFKKESNSLD FDDLIIYFRK LILSSIKVRQ SIHSAYPYIL CDEYQDVNYI
QKDIIDMMDI NNTQMVVGDE GQSIYGWRGS NINYILSFKE NHKNALIYTI RYNYRSGSGI
VKLAENAINL NVEKEENKKI MIPFQKQKVI PVIKGFIDEI EQGNIIANQI LSQKYDLGET
AVLVRSNRLT RDLEFAFRKR HIKYKVVGGT PFYEKAHIKD LFAFLKFEYN KKDRIAFIRI
ITMANGIGAK TAERIYEIFR SCNFNYQFIK NFKVPKKSKD DVETIFNIME ESENIKVTAE
KLKYIFKSFY ENYLYDEYND ESIENRLNDV KKFIEMAEEY ITIDSFIEDL ILDNKNDDDK
SDVVTISTVH RAKGLEWDNV FLPYLNDDVF PSNMSTTIEE LQEEQRLFYV AITRAKKNLF
ISYIDAFSDD PKQNIQYSSK FIKQLDGRLF VAE
//
