ID A0A1S8LG71_9CLOT Unreviewed; 556 AA.
AC A0A1S8LG71;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543,
GN ECO:0000313|EMBL:OOL90896.1};
GN ORFNames=CLAUR_034450 {ECO:0000313|EMBL:URZ03392.1}, CROST_040800
GN {ECO:0000313|EMBL:URZ13321.1}, CROST_09020
GN {ECO:0000313|EMBL:OOL90896.1};
OS Clostridium felsineum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=36839 {ECO:0000313|EMBL:OOL90896.1};
RN [1] {ECO:0000313|EMBL:OOL90896.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 7320 {ECO:0000313|EMBL:OOL90896.1};
RA Poehlein A., Montoya Solano J.D., Flitsch S., Krabben P., Duerre P.,
RA Daniel R.;
RT "Microbial solvent formation.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:URZ13321.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 7320 {ECO:0000313|EMBL:URZ13321.1};
RA Poehlein A., Schoch T., Duerre P., Daniel R.;
RT "Genome sequence of C. roseum typestrain.";
RL Submitted (APR-2022) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:URZ03392.1, ECO:0000313|Proteomes:UP000190175}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 793 {ECO:0000313|EMBL:URZ03392.1,
RC ECO:0000313|Proteomes:UP000190175};
RA Poehlein A., Schoch T., Duerre P., Daniel R.;
RT "Genome sequence of Clostridium aurantibutyricum DSM 793.";
RL Submitted (APR-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
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DR EMBL; LZYV01000041; OOL90896.1; -; Genomic_DNA.
DR EMBL; CP096988; URZ03392.1; -; Genomic_DNA.
DR EMBL; CP096983; URZ13321.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1S8LG71; -.
DR STRING; 84029.CROST_09020; -.
DR KEGG; caun:CLAUR_034450; -.
DR KEGG; crw:CROST_040800; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000190175; Chromosome.
DR Proteomes; UP000190951; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:OOL90896.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}.
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 556 AA; 60337 MW; 9D5F902D5628B6B2 CRC64;
MKTDIEIAQE AKMERISKIA EGIGLGEDDI DLYGKYKCKI SLDVLKKNQD KKDGKLVLVT
AINPTPAGEG KSTVTVGLGE ALCKMDKNTV IALREPSLGP VFGIKGGAAG GGYAQVVPME
DINLHFTGDM HAITSANNLL CAAIDNHIHQ GNNLRIDQRR IVFKRVMDMN DRALRNIVVG
LGGKVNGFPR EDGFMITVAS EIMAILCLAN SLMDLKERMG KILVAYDLDG NPVYCKDLNV
QGAMAMLMKD AMKPNLVQTL ENTPAIIHGG PFANIAHGCN SILATKMALK LGDYVITEAG
FGADLGAEKF LDIKCRYGGL NPDCVVLVAT IRALKHHGGA AKEDLNKPDA KVLEKGLSNL
GKQIENIKKY GVPVVVAINK FITDSDEEIK CIEEYCAKEG VKVSLTEVWE KGGEGGIDLA
KKVLDTLENE KSNFKYLYDE KLSIKEKMDI IAKEIYGADG VQYTPQANKQ IKEIEKFDLD
KLPICVAKTQ YSLSDNPALL GRPSDFTINV KEVRVSNGAG FVVVQTGNIM TMPGLPKVPA
ANKMDIFEDG SIVGLF
//
