ID A0A1S8MHV3_9CLOT Unreviewed; 1915 AA.
AC A0A1S8MHV3;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=Polyketide synthase PksL {ECO:0000313|EMBL:OOL87795.1};
GN Name=pksL {ECO:0000313|EMBL:OOL87795.1};
GN ORFNames=CROST_029090 {ECO:0000313|EMBL:URZ12192.1}, CROST_19110
GN {ECO:0000313|EMBL:OOL87795.1};
OS Clostridium felsineum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=36839 {ECO:0000313|EMBL:OOL87795.1};
RN [1] {ECO:0000313|EMBL:OOL87795.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 7320 {ECO:0000313|EMBL:OOL87795.1};
RA Poehlein A., Montoya Solano J.D., Flitsch S., Krabben P., Duerre P.,
RA Daniel R.;
RT "Microbial solvent formation.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:URZ12192.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 7320 {ECO:0000313|EMBL:URZ12192.1};
RA Poehlein A., Schoch T., Duerre P., Daniel R.;
RT "Genome sequence of C. roseum typestrain.";
RL Submitted (APR-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- PATHWAY: Antibiotic biosynthesis; bacillaene biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004789}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LZYV01000066; OOL87795.1; -; Genomic_DNA.
DR EMBL; CP096983; URZ12192.1; -; Genomic_DNA.
DR STRING; 84029.CROST_19110; -.
DR KEGG; crw:CROST_029090; -.
DR Proteomes; UP000190951; Chromosome.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd19535; Cyc_NRPS; 1.
DR CDD; cd00833; PKS; 2.
DR Gene3D; 1.10.1240.100; -; 2.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF37; FATTY ACID SYNTHASE; 1.
DR Pfam; PF00668; Condensation; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 2.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 2.
DR Pfam; PF00550; PP-binding; 2.
DR SMART; SM00825; PKS_KS; 2.
DR SMART; SM00823; PKS_PP; 1.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 2.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 27..451
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 647..723
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 741..1168
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1393..1468
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT COILED 1350..1377
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1915 AA; 217485 MW; 991F4D3F55161E08 CRC64;
MNLDILNKID ITEFSDEDSS NKNAYKDESI AIIGMSVKAP CADNVEEFWD SLKVGKECVR
AVPQNRKKFN IDYLEYLGYK REEIKFIYAN YIEEIDKFDY EFFNINPKEA ELMDPGQRLF
LQTAWEAIED AGCVEKKLSN SKTGVYVGYG DDSEYLNIIS NIDHDVVGIA QTGNIKPILA
SRLSYLLNLK GPSMIIDTTC SSSLTAIHIA SQALKSKECD MAIAGGVSIR VLPSKNHSSL
GVESKNSRVK TFDDSSNGTL LGEGIGVVIL KTLSKAIEDK DNIYGVIKAS SLNQDGKSSG
ITAPNQIAQE ELITTTWQKA GISPTSLSYI EAHGTGTKLG DPIEIKGIKR AFEMYTDKKQ
FCAIGSIKPN VGHSIQASGV IGVIKVLLMM KYKTIPPLIN FDYPNKSIKF EDSPLFINNK
LRKLDDNNGI IRCGISSFGV SGTNAHMILE KDESLCVKDN DKHCDSYILT LSAKNKESLE
MLVNKYKNML KNRCIDGVGN ICYTANVRRN HYNYRMAIVF KSVEELYEKL SDNKLDGVYY
NKNFLVDKLN KDKLTSEANS LIDNNISSEK LSNIAKLYIE GADVDFSKLY EEKSYKVVHV
PTYQFKKNIC WIKVPKKDIN KEKKDINKLQ QVKFDIDDTI ILSGRSNGLY TRTEKVITTI
LKKLMGINKI GINDRFSDMG GDSIVLTKFH EDLKKVLGIE FSIADIFSYP TIGKLGKYLD
NNFANIENET QNKEPKMVNN DTDIAVIGIS AKLPAADSVE EYWDNMISER ECIVNFPRDR
KKYANKYLRY SGKYDGNVKY IKCGYMKNID EFDWKFFNIS PKEANIMDPH QRLFLQEAWK
TIEDAGYSKE EIAGTKTGVY LGYANDFAFN YWRIVNEVDE ESFKLAVGPN LSSVIPSRIS
YLLDLKGPSM LVDTACASSL VAVHLACQAI LNGECEMALA GGARVEIMPI CNEYNKLGVE
SSSYHLRAFD NSSDGIVWGE GVASLLLKPL KKAREDGDHI YAVIKGSTVN QDGASMGIST
PNPEAQTQMI VDCLKNTNIN PETIDYIECH GTGTEIGDPI EVNSITNAYK QFTSKKQFCG
IGSIKSSIGH CNATSGIAAV IKMVLALKYK ELPASINWEI PNSKINFENS PVYFLNKSVK
WAERSTPRRC AINSFGFSGT NCHVILEEEN SKYKEESDCV NLFVLSSKSA NSLWEYIKSY
SQYLKNNTCN NITSLCYTAS CGRSHYKYRL ALVVKDIKDL ENKINKILDN GYETFIKENN
NDSVEQASYE NIDNFISLFI DNGRRDKMYL EQIARYYKEG AEISWKRLYS DKKYTKVSIP
TYIFEKTKCW VEIPEKNNES YNAVQIIEEI MKKSELSKEL TEELNNVLEE FKENREVNLP
NKKQISIVGR ESNNYTEVEV IVAEIWCDVL GLDEIDINVN FYEVGGHSIA MMQIVSKINK
KLKTNLSYSE FNEQNSVKTL AQMLESKEKS ITQEEYLSIT PDPSSMYDSF PITDIQMSYL
LGRNDSFEMG GVAPQVYMEI ETSLDIKRLN RSLNKLIARH PMLRAVVDNN GTQHILEGEL
KYNIEITDIS QMNNEEIEKY ITEEREKVAL KSFKADEWPL IRIVALKIND KKHHLFIAFD
TLIMDGSSLR IIGDEWISYY KNENLELPKL ELTFRDYMLA LEKFNHSKMY ENDKKYWLEK
LEDFPKAPEL LYKVNPSDIS NPSFKRLSRT YNKENWGKIK DIAKELGVSP SSLLCTAYAE
VLAYWSNQSR FTVSLTVFNR LPFHKDVDKI VGDFTSVMLI PINFNNEESF FDRVKNIHKD
VLNSLEHRSF DGVEMIRKIA VRDKKVGQPV MPIVFTSMLF DGEYNPWSEF GETKIGMSQT
PQVYIDHQAG EIGGELVINW DYVSEIFDKD VINNMFNQYV GILDYLISIN TEEDK
//
