ID A0A1S8T7L5_9CLOT Unreviewed; 3135 AA.
AC A0A1S8T7L5;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE SubName: Full=Gramicidin S synthase 2 {ECO:0000313|EMBL:OOM73736.1};
GN Name=grsB_4 {ECO:0000313|EMBL:OOM73736.1};
GN ORFNames=CLPUN_43700 {ECO:0000313|EMBL:OOM73736.1};
OS Clostridium puniceum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=29367 {ECO:0000313|EMBL:OOM73736.1, ECO:0000313|Proteomes:UP000190890};
RN [1] {ECO:0000313|EMBL:OOM73736.1, ECO:0000313|Proteomes:UP000190890}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 2619 {ECO:0000313|EMBL:OOM73736.1,
RC ECO:0000313|Proteomes:UP000190890};
RA Poehlein A., Montoya Solano J.D., Flitsch S., Krabben P., Duerre P.,
RA Daniel R.;
RT "Microbial solvent formation.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OOM73736.1}.
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DR EMBL; LZZM01000211; OOM73736.1; -; Genomic_DNA.
DR STRING; 29367.CLPUN_43700; -.
DR Proteomes; UP000190890; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR CDD; cd05930; A_NRPS; 2.
DR CDD; cd19543; DCL_NRPS; 1.
DR CDD; cd19534; E_NRPS; 1.
DR CDD; cd19531; LCL_NRPS-like; 2.
DR Gene3D; 3.30.300.30; -; 3.
DR Gene3D; 3.40.50.980; -; 4.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 4.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 4.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR010060; NRPS_synth.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 2.
DR NCBIfam; TIGR01720; NRPS-para261; 1.
DR PANTHER; PTHR45398; ENZYME, PUTATIVE (JCVI)-RELATED; 1.
DR PANTHER; PTHR45398:SF1; ENZYME, PUTATIVE (JCVI)-RELATED; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF13193; AMP-binding_C; 2.
DR Pfam; PF00668; Condensation; 4.
DR Pfam; PF00550; PP-binding; 3.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 8.
DR PROSITE; PS00455; AMP_BINDING; 2.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000190890}.
FT DOMAIN 40..114
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1558..1633
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2598..2673
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 3135 AA; 362818 MW; 46E23FC03B7888EA CRC64;
MIPNYIMQIE ELPVTVNGKV DRKELPEIDV SEIMENEYET ARNETEEYLV KVWEEVLGIE
GIGISHNYYE LGGDSIKSIQ IVSRLQKYNM SLQVKDIMEN PTIKQLSEKV KYKNNEVDQS
VVEGEAELAP IQKWFFENEF SEKNHWNQAF VFYKKDGIKE EILKKSFVEI LKHHDALRMI
YEKDKDKIEQ RNRGLEASED VFTLDAYDLR EDENYKNTIE ELSSKLQEGM NLKKGILVKL
GLFNTKDGDY LLIAIHHMVM DGVSWRILLE DLENLYKAIE NDEEIILPKK TTSYKEWTRK
LLEYANSKEI LKELEYWKSI ENTEIREIPR DFEKTESTLG ESQSISISFS KEETERLLRK
TSAAYNTQIN DILLCSLGLA VKEWSRNDKV LISLEGHGRE EILKDVSIDR TIGWFTSMYP
VILDMTYSED ISYSIQYTKE TLRHIPNNGV GYGILKYLTN NQNNKDNSFK LKPEISFNYL
GEFVYKESKD SFRYSELTGG MAVSTANKKL NSIEINGLVS EGKLELFLNY STKEYKEETV
ANLLEEYKKN LIKVVEHCES KKETQKTPWD YGDNELSIED LNKMLSSEKD IEKIHSLAPM
QEGMLYNLIL DKKSHAYFEQ SVFTLEGTLR VEVLNKVFNT LIEKYEVLRT AFFYGDISKP
KQAVLSKREM LINYEDITTL ADSKQEEYIE NFKRQDRDKG FDLTNDCLIR ISVIKTKDNR
YKLIYSFHHI IMDGWCIGII MRDIVSLYKA FCEGEKNIVI DETVPYSNYL EWLDKQDKAE
ALEYWKVYLS GYEQESVIPK LNNKLTGFNN GEDSIVLDDI LTSEFRKLSE KNNITLNSLI
QTAWAVLLQK YNNTNDVVFG SVISGRPSEI EGIETMMGLF INAVPVRVKG DDNTEFKELA
KKLNNDFIEA NTYGYCSLAE VQSLTTMKNK LINHVVVYEN YPIDKKNMNS GMDKKSEIDM
IDFKTFEQTN YDLQLLIIPS DKLSIKIQYN SNIYSKETIR AIKNNLYNIL NCIIDKPDIK
ISETEMLSLK EQKKILYELN DVEADYPYNK TIKELFEEQV ERTPENIAVI YENTQLTYKE
LNRKANVLAW KLREKGVKEE TIVGIMTNRS AEMIVAILGV LKAGGAYLPI LPDYPKDRIE
YMLEDSQPQV LVGQKELIET VSFKGEILET ESIKEDEDED EKENLVALSS PHSMAYIIYT
SGTTGKPKGV VIENINVVRL MFNSKMQYEF DEKQIWTMFH SYCFDFSVWE MYGALLYGGK
LVVVSDNVTK NPAEFVKLLR KEKVTVLNQT PSSVYNLIEE EMKQEDNNLN IKYITFGGEM
LKPAMLKRWI DKYPDTRLIN MYGITETTVH VTFKEITDNE ITTGISNIGV PIPTTTTYIM
DKNLKLLPIG VPGELCVGGA GVARGYLKRP ELTAEKFVEN PYIKGERIYR SGDLVKMLSN
GEMEYIGRID HQIKIRGFRV EISEIENKLI NHIDIKDAVV IAKDDLTGTK YVCAYITADK
KFTIKELKEY LSQDLPYYMI PSYFVQIDKM PINANGKIDR RLLPEPEENA ILDSSYEAAK
NKIEEKLVAI WEEVLGKEHI GINDNYFDLG GHSLNAAMIT SKINKEFCVD ITVRVLFENP
TINALSRLIE KSEKKESISI YKAGEKEYYK ASSAEQRLFA LWEMDKDNTV YNIPMIFEIN
KELDEKKVQI VLNELIRRHE SLRTKFKIVD DEIVQVVDRE WNLDFKYEEL KETIVNEKIN
NFVKPFDLTK SPLMRCEIIK CSEKKYVFMV DFHHIIVDGM SSSILINEFK ALYEGNVLPE
TALQYKDYSE WQNSLEEKGL IKKQEEYWLN RFKGELTILN ILTDYERSNV QSYEGSRVIF
NIGKALTDNL NKLAKDANTT LYMVLLSAYN VLLAKYSSQE DIIIGTAESG RTKAGLENIV
GMFVNTVALR NYPEGNKTFK EFLNEVKDNT LRDFENADYQ FENLIDKLNI KREASRNPLF
DTMFVLENMD LNSKESSEKI NILEAEFNVS KFDLTLTAME IENGISFNFE YCIKLFKKDT
LEKIKEHFIN ILNSIAAESD IKISEIEMLS LKEQKKILYE LNDVEADYPY DKTIKELFEE
QVERTPENIA VIYENTQLTY KELNRKANVL AWKLREKGVK EETIVGIMTN RSAEMIVAIL
GVLKAGGAYL PILPDYPKDR IEYMLEDSQP QVLVGQKELI ETVSFKGEIL ETESIKEDED
EKENLVALSS PHSMAYIIYT SGTTGKPKGV VIENINVVRL MFNSKMQYEF DEKQIWTMFH
SYCFDFSVWE MYGALLYGGK LVVVSDNVTK NPAEFVKLLR KEKVTVLNQT PSSVYNLIEE
EMKQEDNNLN IKYITFGGEM LKPAMLKRWI DKYPDTRLIN MYGITETTVH VTFKEITDNE
ITTGISNIGV PIPTTTTYIM DKNLKLLPIG VPGELCVGGA GVARGYLKRP ELTAEKFVEN
PYIKGERIYR SGDLVKMLSN GEMEYIGRID HQIKIRGFRV EISEIENKLI NHIDIKDAVV
IAKDDLTGTK YVCAYITADK KFTIKELKEY LSQDLPYYMI PSYFVQIDKM PINANGKIDR
RLLPEPEENA ILDSSYEAPR NMTEEELAFI WEEVLGKEHI GINDNYFDLG GHSLKAAVIV
AKIKKRLCVE IKVRDLFENH TISALSKIIK KLDTKEYIEI SKAEEKEYYK ASSMEKRLFA
LWEINEQDTV YNIPMILEVN EKLDKEKVEK VLIEIIRRHE ALRTSFDIVD GTIVQKIENE
WNLDFKYEEA ENIIVEERIS NFIKPFNLRK GPLMRAEIIK CDEKKYLFMV DFHHIVVDGV
SSIIILNEFI QLYEGKSLPE PLLQYKDYSE WQNSLEESGL IEKQRKYWIN RLGGELPILN
MQTDYERPIV KSNLGGLVNF NVNKELKNNL NKIAKDTGLT LYMILLAAYN IMLSKRSGQE
DIIIGTAQAG RSHEETENLV GMFVNTIVLR NYPKGSKTFK EFLMEVKDST LKDFENTEYQ
FENLIKELDI KRDAGRNPLF DVMFVLENMG SNSGGENKKI KPIEPKLNIS KFDLTLSAVE
TEESIDFRLE YCSSLFRNET VEEMGEHYVN ILNQITSDLD IYISNIEMIS NNEREMYINN
MNNDSEIIDA AEFNF
//
