ID A0A1T1BF69_9DEIO Unreviewed; 903 AA.
AC A0A1T1BF69;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Aconitate hydratase {ECO:0000256|RuleBase:RU361275};
DE Short=Aconitase {ECO:0000256|RuleBase:RU361275};
DE EC=4.2.1.3 {ECO:0000256|RuleBase:RU361275};
GN ORFNames=BXU09_09880 {ECO:0000313|EMBL:OOV14921.1};
OS Deinococcus sp. LM3.
OC Bacteria; Deinococcota; Deinococci; Deinococcales; Deinococcaceae;
OC Deinococcus.
OX NCBI_TaxID=1938608 {ECO:0000313|EMBL:OOV14921.1, ECO:0000313|Proteomes:UP000191141};
RN [1] {ECO:0000313|EMBL:OOV14921.1, ECO:0000313|Proteomes:UP000191141}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LM3 {ECO:0000313|EMBL:OOV14921.1,
RC ECO:0000313|Proteomes:UP000191141};
RA Hentchel K., Vargas G., Fiebig A., Chen S.L., Coleman M., Crosson S.;
RT "Genome sequences of bacteria isolated from the littoral zone of southern
RT Lake Michigan.";
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-
CC aconitate. {ECO:0000256|RuleBase:RU361275}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501,
CC ECO:0000256|RuleBase:RU361275};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185, ECO:0000256|RuleBase:RU361275}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OOV14921.1}.
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DR EMBL; MUFV01000001; OOV14921.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1T1BF69; -.
DR STRING; 1938608.BXU09_09880; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000191141; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01586; AcnA_IRP; 1.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01341; aconitase_1; 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|RuleBase:RU361275};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU361275};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU361275};
KW Lyase {ECO:0000256|RuleBase:RU361275};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 74..566
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 696..823
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 903 AA; 97665 MW; 7CBA56705323741B CRC64;
MAMNLFGARD TLTTQSGQKL YYYNLNKLQE QGHDISRLPV SIKVLLESVL REANDYDVRR
EDVATVAGWK PVNEEVEIPF KPARVILQDF TGVPAVVDLA AMRSAMVKLG GDPSKINPLI
PVDLVIDHSV QVDEFGTDFA LANNMALEFE RNRERYEFLR WGQQAFDNFG VVPPASGIVH
QVNLEYLAKG VQSRPEDDGV VVYPDSLVGT DSHTTMINGL GIVGWGVGGI EAEAVMLGQP
IYMLMPEVIG FKITGAMPEG ATATDLALRV TEMLRAKGVV GKFVEFYGAG LSNMTLPDRA
TIANMAPEYG ATMGFFPVDD EALRYLRRTG RLETEIELVE AYYKAQGMYR TDDTVDPVFT
DTIELDLGTI VPSLAGPKRP QDRVDLTGMH TVFNEALTAP VKARGFELPA DKLDAQGTIT
GTDIKIGHGA VTLASITSCT NTSNPSVLIA AGLVAKKAVE LGLDSKPWVK TSLAPGSRVV
TEYLEAAGLQ TYLDQIGFNT VGYGCMTCIG NSGPLPEPTV DAINEGDLVV ASVLSGNRNF
EGRVNPHIKA NYLASPPLVV AYALAGTVVN DIVNDPIGTS KDGQPVYLRD IWPTAAEIQT
VMDSAINADM FRKVYDGIEK SNQDWNAIPV AEGALYDWKE DSTYIQNPPF FDNLAGGPSD
IVSIEGARAL VKVGDSVTTD HISPAGSFKS DTPAGKFLTE RGILPKDFNS YGSRRGNDRI
MTRGTFANIR LKNQLAPGTE GGFTTDYTTG QVSSIYDASV NYKASNIPLV IFAGKDYGMG
SSRDWAAKGT FLLGVKAVIA ESFERIHRSN LVGMGVLPLQ YKNGESADTL GITGEETFDV
ILPGDLKPRQ DVNVKITAKD GSTRTITVQC RIDTPVEIDY YKNGGILQTV LRGILAKSNE
VKA
//
