ID   A0A1T4K6Q0_VIBCI        Unreviewed;       309 AA.
AC   A0A1T4K6Q0;
DT   10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT   10-MAY-2017, sequence version 1.
DT   24-JAN-2024, entry version 19.
DE   SubName: Full=Pyruvate formate lyase activating enzyme {ECO:0000313|EMBL:SJZ38003.1};
GN   ORFNames=SAMN02745782_00033 {ECO:0000313|EMBL:SJZ38003.1};
OS   Vibrio cincinnatiensis DSM 19608.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=1123491 {ECO:0000313|EMBL:SJZ38003.1, ECO:0000313|Proteomes:UP000190834};
RN   [1] {ECO:0000313|EMBL:SJZ38003.1, ECO:0000313|Proteomes:UP000190834}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 19608 {ECO:0000313|EMBL:SJZ38003.1,
RC   ECO:0000313|Proteomes:UP000190834};
RA   Peterson S.W.;
RL   Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- SIMILARITY: Belongs to the organic radical-activating enzymes family.
CC       {ECO:0000256|ARBA:ARBA00009777}.
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DR   EMBL; FUXB01000001; SJZ38003.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1T4K6Q0; -.
DR   STRING; 1123491.SAMN02745782_00033; -.
DR   OrthoDB; 9782387at2; -.
DR   Proteomes; UP000190834; Unassembled WGS sequence.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR040074; BssD/PflA/YjjW.
DR   InterPro; IPR034457; Organic_radical-activating.
DR   InterPro; IPR012839; Organic_radical_activase.
DR   InterPro; IPR001989; Radical_activat_CS.
DR   InterPro; IPR007197; rSAM.
DR   NCBIfam; TIGR02494; PFLE_PFLC; 1.
DR   PANTHER; PTHR30352:SF14; PYRUVATE FORMATE-LYASE 3-ACTIVATING ENZYME-RELATED; 1.
DR   PANTHER; PTHR30352; PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME; 1.
DR   Pfam; PF13353; Fer4_12; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   PIRSF; PIRSF000371; PFL_act_enz; 1.
DR   SFLD; SFLDG01118; activating_enzymes__group_2; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR   PROSITE; PS01087; RADICAL_ACTIVATING; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Lyase {ECO:0000313|EMBL:SJZ38003.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Pyruvate {ECO:0000313|EMBL:SJZ38003.1};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT   DOMAIN          11..299
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000259|PROSITE:PS51918"
SQ   SEQUENCE   309 AA;  34904 MW;  5987BD77D628BFAC CRC64;
     MYFNIQRFST HDGDGIRSIL FLKGCSLSCP WCQNPESRSE KRSLLFDPRS CMADCQLCVS
     AYQATVNDDE LNDGIRRLNN QITINRQAIT DDQLIALQNV CPTQALSVCG EETQSDELFK
     LLMKDKPFYD QSQGGVTFSG GEPLMQADLV AELAQRLQSN QVSTAIESCM HVPWKNVAKV
     APHIDCWLAD LKHTDEEKFL SWAKGSLKRI KDNFRKLAPI AKRIVIRVPV VPGFNDTLEE
     LHNIIDFAAS LTSCQELHLL PYHTLGINKY RLLDMPYLCS DKPLNKPELL ESAMQYASDH
     TQLNVIVRG
//