ID A0A1T4KBI2_9GAMM Unreviewed; 870 AA.
AC A0A1T4KBI2;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 24-JAN-2024, entry version 19.
DE RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
GN ORFNames=BTE48_05260 {ECO:0000313|EMBL:OPX55974.1};
OS Oceanospirillum multiglobuliferum.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Oceanospirillaceae; Oceanospirillum.
OX NCBI_TaxID=64969 {ECO:0000313|EMBL:OPX55974.1, ECO:0000313|Proteomes:UP000191418};
RN [1] {ECO:0000313|EMBL:OPX55974.1, ECO:0000313|Proteomes:UP000191418}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33336 {ECO:0000313|EMBL:OPX55974.1,
RC ECO:0000313|Proteomes:UP000191418};
RA Carney J.G., Trachtenberg A.M., Rheaume B.A., Linnane J.D., Pitts N.L.,
RA Mykles D.L., Maclea K.S.;
RT "Genome Sequencing of a Marine Spirillum, Oceanospirillum multiglobuliferum
RT ATCC 33336, from Japan.";
RL Submitted (JAN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OPX55974.1}.
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DR EMBL; MTSM01000005; OPX55974.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1T4KBI2; -.
DR STRING; 64969.SAMN02745127_00011; -.
DR OrthoDB; 9764318at2; -.
DR Proteomes; UP000191418; Unassembled WGS sequence.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019679; P:propionate metabolic process, methylcitrate cycle; IEA:InterPro.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR012708; 2Me_IsoCit_deHydtase_FeS-dep.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR02333; 2met_isocit_dHY; 1.
DR PANTHER; PTHR11670:SF43; 2-METHYLCITRATE DEHYDRATASE (2-METHYL-TRANS-ACONITATE FORMING); 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000191418}.
FT DOMAIN 64..540
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 664..795
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 870 AA; 94834 MW; 7FCC2318D87F51F2 CRC64;
MNTEYRKSLS GTSLDYFDTR AAVEAIQPGA YATLPYTSRI LAEQLVRRCE PEALTDSLKQ
LIERKRDLDF PWYPARVVCH DILGQTALVD LAGLRDAIAE QGGDPSKVNP VVPTQLIVDH
SLAVEAAGFD PDAFEKNRAI EDRRNDDRFH FIEWCKTAFD NVDVIPAGNG IMHQINLEKM
SPVIQARDGV AFPDTCVGTD SHTPHVDALG VVAIGVGGLE AETVMLGLPS MMRLPDIIGV
ELVGKRQPGI TATDIVLAIT EFLRNEKVVS AYLEFFGEGA KALSIGDRAT ISNMTPEFGA
SAGMFYIDEQ TIEYLKLTGR EPEQVALVEQ YAKETGLWAD DLATAEYERV LKFDLSTVVR
NMAGPSNPHR RLPTSDLASR GIAGDAMLAS AKAEETEGLM PDGAVIIAAI TSCTNTSNPR
NVVAAGLVAK KANELGLVRK PWVKSSFAPG SKVAKLYLEK AGLLSEMEKL GFGIVAYACT
TCNGMSGALD PVIEKEIIDR DLYATAVLSG NRNFDGRIHP HAKQAFLASP PLVVAYAIAG
TIRFDIEKDV LGTDQAGNPV TLKDIWPSDE EIDTLVEQSV TPEQFKQVYI PMFDLGAVER
SKSPLYDWRP QTTYIRRPPY WEGALAGERT MKGMVPLAVL GDNITTDHLS PSNAIQASSA
AGEYCAKMGL PEEDFNSYAT HRGDHLTAQR ATFANPKLLN EMCRDENGNV KQGSLARIEP
EGVESRMWEA IETYMERKQP LIIIAGADYG QGSSRDWAAK GVRLAGVEAI VAEGFERIHR
TNLVGMGVLP LEFKAGETRM TYGIDGTETY DVVGDLSPRT DLTVVMTRRN GEQVSIPVTC
RLDTAAEVSV YQAGGVLQRF AKDFLESNAN
//
